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We narrowed to 3,539 results for: lenti crispr cas9 plasmids

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Showing: 121 - 160 of 3539 results

  1. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid
    #106477
    Purpose
    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106477-plasmid-map-sequence-id-207983

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  4. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid
    #106964
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    106964-plasmid-map-sequence-id-207987

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  5. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid
    #106965
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106965-plasmid-map-sequence-id-207982

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  6. pTRE3G-VP64-dCas9-VP64-ZsG

    Plasmid
    #192668
    Purpose
    Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    VP64-dCas9-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    TRE3G
    Available Since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192668-plasmid-map-sequence-id-379892

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  7. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid
    #102244
    Purpose
    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter
    Depositor
    Jacob Corn
    Insert
    dCas9-HA-BFP-KRAB-NLS
    Use
    CRISPR and Lentiviral
    Tags
    HA-TagBFP-KRAB-NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102244-plasmid-map-sequence-id-197289

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  8. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83481-plasmid-map-sequence-id-335118

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  9. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Jacob Corn
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102245-plasmid-map-sequence-id-354473

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  10. hPLD3-sgRNA-Cas9_mcherry

    Plasmid
    #199342
    Purpose
    encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Promoter
    human U6
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199342-plasmid-map-sequence-id-390619

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  11. hPLD4-sgRNA-Cas9_mcherry

    Plasmid
    #199343
    Purpose
    encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-mCherry
    Expression
    Mammalian
    Mutation
    sgRNA: accagtagtatgaagccacg
    Promoter
    human U6
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199343-plasmid-map-sequence-id-390620

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  12. pLV-TET2BlastR-Cas9-TagBFP

    Plasmid
    #236726
    Purpose
    Dox Inducible Cas-TagBFP expression
    Depositor
    David Bersten
    Insert
    Cas9-TagBFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    May 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    236726-plasmid-map-sequence-id-474177

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  14. pDual_dsCas9_Puro_hs_NC_chr1

    Plasmid
    #214688
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Mutation
    Puromycin resistance cassette has silent mutation…
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214688-plasmid-map-sequence-id-428817

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  15. pDual_dsCas9_Puro_hs_TP73

    Plasmid
    #214689
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Mutation
    Puromycin resistance cassette has silent mutation…
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214689-plasmid-map-sequence-id-428791

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  16. pTRE3G-dCas9-VP64-MS2-VP64-ZsG

    Plasmid
    #192669
    Purpose
    Dox-inducible expression of dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    TRE3G
    Available Since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192669-plasmid-map-sequence-id-379887

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  17. mPLD3-sgRNA-Cas9-mcherry

    Plasmid
    #199700
    Purpose
    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199700-plasmid-map-sequence-id-390617

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  18. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

    Plasmid
    #221385
    Purpose
    Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene
    Depositor
    Junwei Shi
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    221385-plasmid-map-sequence-id-435944

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  19. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

    Plasmid
    #117134
    Purpose
    All-in-one expression vector for Cas9 and gRNA against CD44
    Depositor
    Kosuke Yusa
    Insert
    gCD44 v2 (Cd44 Synthetic)
    Use
    CRISPR and Lentiviral
    Mutation
    Deleted BbsI site within WPRE, modified BFP
    Promoter
    human U6 promoter, human EF1a promoter
    Available Since
    April 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    117134-plasmid-map-sequence-id-226805

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  20. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid
    #196720
    Purpose
    Tet-inducible expression of Cas9-T2A-TagBFP
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Promoter
    TRE, PGK
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196720-plasmid-map-sequence-id-389756

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  21. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid
    #196722
    Purpose
    Tet-inducible expression of Cas9-T2A-miRFP670nano
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Promoter
    TRE, PGK
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196722-plasmid-map-sequence-id-389804

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  23. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90085-plasmid-map-sequence-id-182691

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  27. Lenti sgRNA NGFR CFP out of frame

    Plasmid
    #155283
    Purpose
    Lentiviral plasmid for sgRNA, NGFR marker, editing detected with CFP, used with 155280
    Depositor
    Martin Carroll
    Type
    Empty backbone
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155283-plasmid-map-sequence-id-309991

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  28. SIN-cPPT-G1B3-spCas9-WPRE-miR124T

    Plasmid
    #245071
    Purpose
    CRISPR Editing with SpCas9, with miRNA-124 target sequence to repress transgene expression in neurons
    Depositor
    Nicole Déglon
    Insert
    Cas9, miR124T site
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Human G1B3 promoter
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245071-plasmid-map-sequence-id-495712

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  31. Lenti sgRNA NGFR GFP out of frame

    Plasmid
    #155282
    Purpose
    Lentiviral plasmid for sgRNA, NGFR marker, editing detected with GFP, used with 155280
    Depositor
    Martin Carroll
    Insert
    GFP out of frame IRES NGFR
    Available Since
    Sept. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    155282-plasmid-map-sequence-id-310437

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  33. lenti MS2-P65-HSF1_Hygro

    Plasmid
    #61426
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Insert
    MS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1A
    Available Since
    Dec. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    61426-plasmid-map-sequence-id-140565

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  35. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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  36. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73795-plasmid-map-sequence-id-181202

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Showing: 121 - 160 of 3539 results