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Showing: 151 - 200 of 521 results

  15. pAL612

    Plasmid
    #119860
    Purpose
    precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Cysterine 290 to Serine and Glycine 244 to Cystei…
    Promoter
    lac
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119860-plasmid-map-sequence-id-232053

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  16. pFBCPDL6

    Plasmid
    #67285
    Purpose
    CPD photolyase from Drosophila with Flag tag in pFastbac vector. Intermediate in insect cell expression
    Depositor
    Aziz Sancar
    Article
    Selby et al Biochemistry. 2012 Jan 10;51(1):167-71.
    Insert
    CPD photolyase (phr Fly)
    Use
    Bacterial vector, insert not expressed
    Tags
    Flag
    Expression
    Mutation
    V275E*
    Promoter
    Available since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  18. pAL725

    Plasmid
    #119862
    Purpose
    precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Leucine 244 to Cysteine, Aspartate 287 to Cysteine
    Promoter
    T5
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119862-plasmid-map-sequence-id-232032

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  22. pAL942

    Plasmid
    #119721
    Purpose
    precursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 Cysteine
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor slow-folding ribose import binding protein, rbsB, mutations A248T, A118C and E246C
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Alanine 248 to Threonine, Alanine 188 to Cysteine…
    Promoter
    T7
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119721-plasmid-map-sequence-id-232465

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  25. pHR-AL-mCh-Cry2WT-NLS

    Plasmid
    #221924
    Purpose
    Expression of CBP autoregulatory loop (AL) fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP AL (aa 1529-1607) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the autoregulatory loop (AL) (aa 152…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221924-plasmid-map-sequence-id-437707

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  28. pQE80l BCB

    Plasmid
    #196472
    Purpose
    Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher
    Depositor
    Fei Sun
    Article
    Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114
    Insert
    BCB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196472-plasmid-map-sequence-id-387266

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  30. pAL829

    Plasmid
    #119697
    Purpose
    precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutations Y283D, G289C and V347C
    Promoter
    T5
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119697-plasmid-map-sequence-id-232493

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  31. pUNC2001

    Plasmid
    #67280
    Purpose
    Expresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcw
    Depositor
    Aziz Sancar
    Article
    Worthington et al J Biol Chem. 2003 Oct 3;278(40):39143-54
    Insert
    Vibrio cholera photolyase
    Use
    Tags
    maltose binding protein
    Expression
    Bacterial
    Mutation
    none known
    Promoter
    Available since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  32. GFP-C1-PLCdelta-PH

    Plasmid
    #21179
    Purpose
    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids
    Depositor
    Tobias Meyer
    Article
    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.
    Insert
    PLCdelta (PLCD1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    PH domain of human PLC delta, amino acids Met1–Il…
    Promoter
    Available since
    July 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21179-plasmid-map-sequence-id-212104

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  38. pCEC-red

    Plasmid
    #196040
    Purpose
    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly
    Depositor
    Paola Branduardi
    Article
    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Grna cloning
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196040-plasmid-map-sequence-id-386150

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  39. pLenti-TetO-PM

    Plasmid
    #197387
    Purpose
    To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)
    Depositor
    Qiao Zhou
    Article
    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038
    Insert
    PDX1-T2A-MAFA
    Use
    Lentiviral; Teto
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    197387-plasmid-map-sequence-id-385579

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  49. pMJB1-RPS22B-Cl

    Plasmid
    #160431
    Purpose
    Expresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoter
    Depositor
    Gary Stormo
    Article
    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:
    Insert
    RPS22B (RPS22B Budding Yeast)
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Removed introns, shuffled codons to change nucleo…
    Promoter
    GAL1 and S. cerevisiae GAL1
    Available since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    160431-plasmid-map-sequence-id-317512

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Showing: 151 - 200 of 521 results