We narrowed to 2,981 results for: erf

Showing: 61 - 80 of 2981 results

Results per page:

Sort results by:

pLB

Plasmid

#11619

Purpose

pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.

Depositor

Stephan Kissler

Article

Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Tags

GFP

Expression

Mammalian

Promoter

mouse U6

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAX FLAG APP

Plasmid

#30154

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein (APP Human)

Tags

FLAG

Expression

Mammalian

Mutation

wild type splice variant 751

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pSico PGK puro

Plasmid

#11586

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-NDi-CRISPRi (Gen1)

Plasmid

#73497

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

dCas9-KRAB-P2A-mCherry
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
sfGFP-N1

Plasmid

#54737

Purpose

Localization: N1 Cloning Vector, Excitation: 485, Emission: 507

Depositor

Michael Davidson, Geoffrey Waldo

Article

Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

Type

Empty backbone

Tags

sfGFP

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
sfGFP-pBAD

Plasmid

#54519

Purpose

Localization: Protein Expression Vector , Excitation: 485, Emission: 507

Depositor

Michael Davidson, Geoffrey Waldo

Article

Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

Type

Empty backbone

Tags

6xHis and SfGFP

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLV-tTRKRAB-red

Plasmid

#12250

Depositor

Didier Trono

Article

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Insert

TetR-KRAB fusion and dsRED

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Mutation

dsRed added to backbone.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-YFP

Plasmid

#11180

Purpose

Mammalian expression vector for expression of YFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

EYFP from Aequorea victoria

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-sfGFP

Plasmid

#85492

Purpose

pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

Depositor

Ryan Mehl

Article

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFlagCMV2-EFP

Plasmid

#12449

Depositor

Dong-Er Zhang

Article

Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

Insert

EFP (TRIM25 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER11 (miR-RUG)

Plasmid

#44363

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-NDi-CRISPRi (Gen2)

Plasmid

#73498

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

dCas9-KRAB
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-jGCaMP7f

Plasmid

#104483

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7f

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pC13N-dCas9-BFP-KRAB

Plasmid

#127968

Purpose

constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-dCas9-NLS-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIG-w

Plasmid

#12282

Purpose

a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus

Depositor

Luk Parijs

Article

Refaeli et al J Exp Med. 2002 Oct 7. 196(7):999-1005.

Type

Empty backbone

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Available Since

July 28, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

Plasmid

#180263

Purpose

Lentiviral expression vector for dCas9-VP64 in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK-Venus

Plasmid

#25734

Purpose

Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
4XCLEAR-luciferase reporter

Plasmid

#66800

Purpose

Reporter for activity of the CLEAR network

Depositor

Albert La Spada

Article

Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

Insert

4XCLEAR

Use

Luciferase

Promoter

Four CLEAR elements (GTCACGTGAC) in tandem derive…

Available Since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-hAgo2

Plasmid

#11590

Depositor

Edward Chan

Article

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

Ago2 (AGO2 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 30, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pRT029

Plasmid

#127969

Purpose

double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge