We narrowed to 2,857 results for: BFP

1. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

   Plasmid

   #171090

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Sth1 dCas9
   Chloramphenicol acetyltransferase
   TagBFP
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

   Expression

   Mutation

   D9A, H599A

   Promoter

   TUB1 (Toxoplasma gondii), transcriptionally linke…

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR

   Plasmid

   #126697

   Purpose

   donor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

   Insert

   TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. EK0211 CMV-TO-BFPmut-stop-t17d9b (FLP-IN)

   Plasmid

   #191139

   Purpose

   Expression of Trigger 2 in 3' UTR of an inactive BFP in mammalian cells. Used as second input in OR, AND gates

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   t17d9b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV-TO

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LV anti-FITC-LaG16-Y186F-T2A-BFP

   Plasmid

   #194931

   Purpose

   LV construct encoding Y186F mutant sNRR-based SynNotch receptor

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   antiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LV anti-FITC-LaG16-Y186A-T2A-BFP

   Plasmid

   #194932

   Purpose

   LV construct encoding Y186A mutant sNRR-based SynNotch receptor

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   antiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)

   Plasmid

   #156409

   Purpose

   pCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12D

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4A-G12D (KRAS Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   mutant KRAS4A-G12D

   Promoter

   CMV

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP

   Plasmid

   #31477

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   SFFV HA NLS Y2 T2A mTagBFP
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Expression

   Mutation

   F13Y + S111Y on I-AniI = Y2 "reo" (res…

   Promoter

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. bactin-loxp-BFP-loxp-dsred-pBSII

   Plasmid

   #68972

   Purpose

   for transgenic animals

   Depositor

   David Traver

   Article

   Kobayashi et al Nature. 2014 Aug 21;512(7514):319-23. do

   Inserts

   lox-BFP-loxp
   dsred
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

   Plasmid

   #187089

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Pre-SPHERE-SF-iGluSnFR
   

   Use

   Tags

   TagBFP-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-iGABASnFR

    Plasmid

    #187091

    Purpose

    GABA sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-iGABASnFR
    

    Use

    Tags

    TagBFP-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. LV anti-FITC-LaG16-R103A-T2A-BFP

    Plasmid

    #194935

    Purpose

    LV construct encoding R103A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-mir144 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164791

    Purpose

    Express miR-144 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144 (MIR144 Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

    Plasmid

    #120268

    Purpose

    Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

    Depositor

    Edward Callaway

    Article

    Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

    Insert

    EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

    Plasmid

    #71213

    Purpose

    dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A
    

    Use

    Tags

    dSpCas9-BFP-DNMT3A(E752A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHBS1504 [IBB-GFP-mCherry3E]-[BFP-TDP43 F-S]

    Plasmid

    #133328

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All F to S in CTD of full-length TDP43

    Promoter

    Available since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

    Plasmid

    #102594

    Purpose

    Lentivector to express STING V155M delta 342 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    V155M delta 342

    Promoter

    SFFV

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

    Plasmid

    #164800

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA1
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

    Plasmid

    #164801

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-Ef1a-Con/Foff 2.0-BFP

    Plasmid

    #137130

    Purpose

    Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-BFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. MADR pDonor TagBFP2-3XFlag (cyto) WPRE

    Plasmid

    #129421

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-3Flag
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    3X Flag epitope tag

    Expression

    Mutation

    Promoter

    none (3X polyA to mitigate episomal expression)

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

    Plasmid

    #164046

    Purpose

    CRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA gene

    Depositor

    Baohui Chen

    Article

    Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

    Insert

    mTagBFG-TriTag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pU6-mir451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164792

    Purpose

    Express miR-451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    microRNA 451a (MIR451A Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

    Plasmid

    #31478

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV HA NLS Y2 K227M T2A BFP
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    F13Y, S111Y, K227M = Y2 nickase "reo" …

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

    Plasmid

    #71214

    Purpose

    dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Use

    Tags

    dSpCas9-BFP-DNMT3B(E697A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LV anti-FITC-LaG16-Y192A-T2A-BFP

    Plasmid

    #194933

    Purpose

    LV construct encoding Y192A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y192A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RAB11A-BFP HDRT Source (pTR 145)

    Plasmid

    #112014

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-BFP HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2

    Plasmid

    #128166

    Purpose

    Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    mTagBFP2-T2A-sfGFP-iCre-ERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHBS1527 [IBB-GFP-mCherry3E]-[BFP-TDP43 W-S]

    Plasmid

    #133333

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All W to S in CTD of full-length TDP43

    Promoter

    Available since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTRIP-SFFV-mtagBFP-2A STING V155M 354STOP

    Plasmid

    #102595

    Purpose

    Lentivector to express STING V155M delta 354 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    V155M delta 354

    Promoter

    SFFV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CD4-C-BFP HDRT Source (pTR 176)

    Plasmid

    #112020

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-BFP HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge