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Showing: 1951 - 2000 of 18428 results

  1. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

    Plasmid
    #135669
    Purpose
    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    sgPten
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135669-plasmid-map-sequence-id-325841

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  3. pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut

    Plasmid
    #173167
    Purpose
    Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutation
    Depositor
    Kivanc Birsoy
    Article
    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi
    Insert
    Calcineurin Like EF-Hand Protein 1 (CHP1 Human)
    Use
    Retroviral
    Tags
    3XFLAG
    Expression
    Mutation
    Synonymous mutations at sgRNA sites, G2A, S6A
    Promoter
    MMLV
    Available since
    Aug. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173167-plasmid-map-sequence-id-341376

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  4. Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G

    Plasmid
    #157982
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Tetra-8A8G)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    157982-plasmid-map-sequence-id-332394

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  17. pRDAX-EMMI-021 IRES-D[C333G](H-I)//Esp3I

    Plasmid
    #130223
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    IRES-D[C333G]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    C333G
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130223-plasmid-map-sequence-id-249601

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  18. pRDAX-EMMI-023 IRES-F[C336A](H-I)//Esp3I

    Plasmid
    #130225
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    IRES-F[C336A]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    C336A
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130225-plasmid-map-sequence-id-249603

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  19. pRDAX-EMMI-018 IRES-A[G338A](H-I)//Esp3I

    Plasmid
    #130220
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    IRES-A[G338A]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    G338A
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130220-plasmid-map-sequence-id-249598

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  20. pRDAX-EMMI-019 IRES-B[C335T](H-I)//Esp3I

    Plasmid
    #130221
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    IRES-B[C335T]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    C335T
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130221-plasmid-map-sequence-id-249599

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  21. pRDAX-EMMI-020 IRES-C[A334G](H-I)//Esp3I

    Plasmid
    #130222
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    IRES-C[A334G]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    A334G
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130222-plasmid-map-sequence-id-249600

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  26. IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA

    Plasmid
    #121748
    Purpose
    pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    OLLAS epitope tag-IRES2-eGFP + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121748-plasmid-map-sequence-id-237186

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  31. pCru5-CCC/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49222
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    Viral LTR (or CMV)
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49222-plasmid-map-sequence-id-73633

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  32. pWPT-CCC/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49231
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49231-plasmid-map-sequence-id-73651

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  33. pWPT-GGG/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49230
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49230-plasmid-map-sequence-id-73649

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  34. pCru5-ACC/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49220
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    Viral LTR (or CMV)
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49220-plasmid-map-sequence-id-73629

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Showing: 1951 - 2000 of 18428 results