We narrowed to 6,177 results for: nls

1. Dendra2-PCNA-19-SV40NLS-4

   Plasmid

   #57735

   Purpose

   Localization: Nucleus, Excitation: 490 / 553, Emission: 507 / 573

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Tags

   Dendra2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. mTFP1-PCNA-19-SV40NLS-4

   Plasmid

   #55505

   Purpose

   Localization: Nucleus, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Tags

   mTFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. GFP-Drosha270-1374delNLS2

   Plasmid

   #62532

   Purpose

   N-terminal aa1-269 and nuclear localization signal 2 of Drosha are deleted

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   truncated human Drosha mutant (DROSHA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-269 and nuclear localizatio…

   Available Since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

4. mKikGR-PCNA-19-SV40NLS-4

   Plasmid

   #57328

   Purpose

   Localization: Nucleus, Excitation: 507 / 583, Emission: 517 / 593

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Tags

   mKikGR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. 1xNLS-pMJ915v2-sfGFP

   Plasmid

   #88919

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. mKO2-PCNA-19-SV40NLS-4

   Plasmid

   #57890

   Purpose

   Localization: Nucleus, Excitation: 551, Emission: 565

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Tags

   mKO2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2xNLS-pMJ915v2-sfGFP

   Plasmid

   #88920

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-BE3-FNLS

   Plasmid

   #110836

   Purpose

   CMV expression vector for BE3-FNLS construct (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3-FNLS
   

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   NLS sequence at the N-terminus

   Promoter

   CMV

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEBG-TFII-deltaNLS1

   Plasmid

   #22153

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   deletion of amino acids 297-304

   Available Since

   Nov. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEmerald-PCNA-19-SV40NLS-4

    Plasmid

    #54223

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Tags

    3xSV40NLS and mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

11. mOrange2-PCNA-19-SV40NLS-4

    Plasmid

    #57971

    Purpose

    Localization: Nucleus, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Tags

    mOrange2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pBSII-NLS-mEmerald(AM)

    Plasmid

    #244811

    Purpose

    In-Vitro Synthesis of mRNA encoding a Honeybee-optimized mEmerald-labeled Nuclear Localization Signal (NLS) Tag

    Depositor

    Frederic Strobl

    Insert

    mEmerald
    

    Use

    In-vitro mrna synthesis

    Tags

    SV40 NLS

    Mutation

    coding sequence is optimized for the Honeybee

    Promoter

    T7

    Available Since

    Oct. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBSII-NLS-mCherry(AM)

    Plasmid

    #244814

    Purpose

    In-Vitro Synthesis of mRNA encoding a Honeybee-optimized mCherry-labeled Nuclear Localization Signal (NLS) Tag

    Depositor

    Frederic Strobl

    Insert

    mCherry
    

    Use

    In-vitro mrna synthesis

    Tags

    SV40 NLS

    Mutation

    coding sequence is optimized for the Honeybee

    Promoter

    T7

    Available Since

    Oct. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBSII-NLS-mTagBFP(AM)

    Plasmid

    #244808

    Purpose

    In-Vitro Synthesis of mRNA encoding a Honeybee-optimized mTagBFP2-labeled Nuclear Localization Signal (NLS) Tag

    Depositor

    Frederic Strobl

    Insert

    mTagBFP2
    

    Use

    In-vitro mrna synthesis

    Tags

    SV40 NLS

    Mutation

    coding sequence is optimized for the Honeybee

    Promoter

    T7

    Available Since

    Oct. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. PS Intein NLS-NES

    Plasmid

    #242021

    Purpose

    Blue-light-induced reconstitution of mCherry and its relocalization to the nucleus.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    PS Intein NLS-NES
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEM8_2CTH10_Bac_Lbm6 (LbCas12a)-4NLS

    Plasmid

    #244843

    Purpose

    Bacterial expression of Lbm6-LbCas12a with 10xHis-MBP at N-temrinus and 4xSV40 NLS at C-temrinus

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    Lbm6-Cas12a
    

    Use

    CRISPR

    Tags

    10xHis-MBP and 4xSV40 NLS

    Expression

    Bacterial

    Mutation

    D535G/S551F/D665N

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT3TS-RfxCas13d-2C-NLS

    Plasmid

    #239816

    Purpose

    Plasmid to carry out IVT of RfxCas13d with SV40 and nucleoplasmin long-NLP NLS (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

    Insert

    Rfx-Cas13d
    

    Use

    CRISPR

    Tags

    Nucleoplasmin long NLS and SV40 NLS

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTcTurboID-GW_GFP-NLS

    Plasmid

    #234716

    Purpose

    Used to express GFP-3xNLS fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Used as proximity labelling nuclear spatial control.

    Depositor

    Esteban Serra

    Insert

    GFP-3xNLS
    

    Use

    Trypanosomatid expression

    Tags

    3xHA

    Promoter

    T7

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-TDP-43 (PY-NLS)

    Plasmid

    #235490

    Purpose

    Expression of chimeric human TDP-43-EGFP bearing a PY-nuclear localization sequence (NLS) from HNRNPA1 in place of the native NLS sequence

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Replaced native NLS with PY-NLS from HNRNPA

    Available Since

    May 19, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. UAST-uMCas12a+nls2x

    Plasmid

    #230909

    Purpose

    inducible expression of Cas12a+ with Gal4.

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    Cas12a+
    

    Use

    CRISPR

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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