We narrowed to 3,430 results for: Agt

1. pX330.LoxPO

   Plasmid

   #66585

   Purpose

   sgRNA for LSL

   Depositor

   Wen Xue

   Article

   Li et al Genome Biol. 2015 May 28;16(1):111.

   Insert

   LSL-Tomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

2. PM-ST1!TB

   Plasmid

   #48654

   Purpose

   Bacterial ST1 crRNA expression: targets ST1 to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Bacterial ST1 crRNA to prototspacer B
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   J23100 promoter

   Available since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-blast-cfRab25_2

   Plasmid

   #26711

   Depositor

   Keith Mostov

   Article

   Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

   Insert

   dog Rab25 RNAi
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   RNAi to canine Rab25.

   Promoter

   Available since

   Dec. 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pABE-dGFP-gRNA

   Plasmid

   #226864

   Purpose

   Human gRNA expression vector targeting the A111V mutation of non-fluorescent EGFP in plasmid pABE-dGFP; for use with ABEs

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   Sp-gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. OgeuIscB ωRNA-v2

   Plasmid

   #220958

   Purpose

   human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

   Insert

   OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221 msNLRP3 (STOP)

   Plasmid

   #218638

   Purpose

   Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (Nlrp3 Mouse)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-ZWILCH gRNA1

   Plasmid

   #211634

   Purpose

   sgRNA-1 against ZWILCH

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   sgRNA ZWILCH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-ERCC6L gRNA1

   Plasmid

   #211632

   Purpose

   sgRNA-1 against ERCC6L

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   sgRNA ERCC6L
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti-ERCC1 gRNA2

   Plasmid

   #211631

   Purpose

   sgRNA-2 against ERCC1

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   sgRNA ERCC1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDT4

    Plasmid

    #199120

    Purpose

    scrambled promoter circular DNA template

    Depositor

    Jeff Gelles

    Article

    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

    Insert

    synthetic transcription template
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …

    Promoter

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. RP51A-NoBS-NoPseudoBS

    Plasmid

    #100986

    Purpose

    RP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BS

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    BS mutated to GTTAGTG, pseudo BS mutated to GTTTG…

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEDJ8

    Plasmid

    #177271

    Purpose

    Double gRNA for RNH1 RNH201 knock-out

    Depositor

    Michael Jensen

    Article

    Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

    Insert

    gRNA_RNH1_RNH201
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pISKP1-TIR1

    Plasmid

    #165061

    Purpose

    Integrative expression of SKP1-fusing rice auxin receptor gene OsTIR1

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1- T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pILGFP105

    Plasmid

    #165060

    Purpose

    Integrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1- T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pILGFP104

    Plasmid

    #165059

    Purpose

    Integrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJAID9L104

    Plasmid

    #165054

    Purpose

    Integrative expression of yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-FLEX-SaCas9-U6-sgDcc

    Plasmid

    #159906

    Purpose

    Mutagenesis of Dcc

    Depositor

    Larry Zweifel

    Insert

    Dcc (Dcc Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLL3.7-Rictor-shRNA

    Plasmid

    #157930

    Purpose

    Knockdown of Rictor

    Depositor

    Baoji Xu

    Article

    Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

    Insert

    Rictor shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    mouse U6

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSUI-SynP-CANTsg

    Plasmid

    #154343

    Purpose

    Expression of the sgRNA targeting to the E. coli can gene

    Depositor

    I-Son Ng

    Article

    Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

    Insert

    CANT sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRBPJ.1.0-gDNA

    Plasmid

    #132474

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    RBPJ (RBPJ Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PX458_ZBTB1_1

    Plasmid

    #128242

    Purpose

    Encodes gRNA for 3' target of human ZBTB1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZBTB1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PX458_THRA_iso1_1

    Plasmid

    #128245

    Purpose

    Encodes gRNA for 3' target of human THRA_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    THRA_iso1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PX330_SSX2B

    Plasmid

    #128252

    Purpose

    Encodes gRNA for 3' target of human SSX2B

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    SSX2B gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pE2F5.1.0-gDNA

    Plasmid

    #112447

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor E2F5

    Depositor

    Kevin White

    Insert

    E2F5 (E2F5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLKO.1P shNfs1

    Plasmid

    #102967

    Purpose

    Suppression of mNfs1

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shNfs1
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCR-U6-gRNA-miniCMV-TdTomato

    Plasmid

    #192656

    Purpose

    A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    TdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, miniCMV

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV-shRNA-ctrl

    Plasmid

    #85741

    Purpose

    shRNA ctrl

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA ctrl
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAS9-mCherry-Frame +0

    Plasmid

    #66939

    Purpose

    CRISPaint frame selector +0

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +0
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAS9-mCherry-Frame +2

    Plasmid

    #66941

    Purpose

    CRISPaint frame selector +2

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAS9-mCherry-Frame +1

    Plasmid

    #66940

    Purpose

    CRISPaint frame selector +1

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge