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We narrowed to 7,347 results for: alp;

Showing: 2001 - 2050 of 7347 results

  1. pCAG-2dV-Camui (wt)

    Plasmid
    #220366
    Purpose
    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)
    Depositor
    Ryohei Yasuda
    Article
    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-
    Insert
    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    Use
    Tags
    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    220366-plasmid-map-sequence-id-434512

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  3. TFORF0480

    Plasmid
    #143705
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HIF1A (HIF1A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    143705-plasmid-map-sequence-id-409081

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  6. APP-bio-His

    Plasmid
    #51643
    Purpose
    Expresses full-length Amyloid beta A4 protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    APP (APP Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51643-plasmid-map-sequence-id-78965

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  10. pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid
    #176460
    Purpose
    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Article
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Insert
    HH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176460-plasmid-map-sequence-id-346305

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  17. pWZL-Blast FAP S624A

    Plasmid
    #207402
    Purpose
    Expresses the S624A mutant of FAP (Fibroblast Activation Protein), which shows no protease activity.
    Depositor
    Jing Yang
    Article
    Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi
    Insert
    Fibroblast Activation Protein Alpha (FAP Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    changed Serine 624 to Alanine. It's a protea…
    Promoter
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207402-plasmid-map-sequence-id-414821

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  18. pWZL-Blast FAP A657S

    Plasmid
    #207403
    Purpose
    Expresses the A657S mutant of FAP (Fibroblast Activation Protein), which shows partial protease activity.
    Depositor
    Jing Yang
    Article
    Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi
    Insert
    Fibroblast Activation Protein Alpha (FAP Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    changed Alanine 657 to Serine. It's an endop…
    Promoter
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207403-plasmid-map-sequence-id-414820

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  19. pCAG CAPN7 V18D,F98D-OSF

    Plasmid
    #193045
    Purpose
    Mammalian expression plasmid encoding full-length CAPN7 with V18D and F98D mutations that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    CAPN7 (CAPN7 Human)
    Use
    Tags
    OSF (One-Strep-FLAG)
    Expression
    Mammalian
    Mutation
    V18D, F98D
    Promoter
    Chicken Beta-actin
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193045-plasmid-map-sequence-id-378994

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  28. TFORF3297

    Plasmid
    #144773
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HIF1A (HIF1A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144773-plasmid-map-sequence-id-404361

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  34. pCDNA3.1-nAChr-beta3-YFP

    Plasmid
    #50484
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    YFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. pT3-EF1aH-PIK3CA H1047R

    Plasmid
    #180031
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.
    Depositor
    Xin Chen
    Article
    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv
    Insert
    PIK3CA H1047R (PIK3CA Human)
    Use
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    H1047R
    Promoter
    EF1a
    Available since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180031-plasmid-map-sequence-id-357864

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  45. TFORF3296

    Plasmid
    #144772
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HIF1A (HIF1A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144772-plasmid-map-sequence-id-404363

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  49. pCDNA3.1-nAChr-beta3-CFP

    Plasmid
    #50485
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    CFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 2001 - 2050 of 7347 results