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pLenti-xF2X-P2A-Puro

Plasmid

#110873

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS-2X(3.7)

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGG>nls-hCas9-nls

Plasmid

#99138

Purpose

Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

Depositor

Marianne Bronner

Publication

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized Cas9

Use

CRISPR

Promoter

Chicken beta actin (CAG)

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-HA-RAPGEF2

Plasmid

#110162

Purpose

Expression of human RAPGEF2 in mammalian cells

Depositor

Daniele Guardavaccaro

Publication

Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

Insert

RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-BFP

Plasmid

#107722

Purpose

For expression of sgRNA from the U6 promoter with BFP expression simultaneously

Depositor

Xingxu Huang

Publication

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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iRFP-Sec61beta

Plasmid

#108125

Purpose

ER marker

Depositor

Gerry Hammond

Publication

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iRFP:SEC61B (SEC61B Human)

Expression

Mammalian

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3 1 kb prom. + Enhancer CD274

Plasmid

#107005

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Publication

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Mutation

N.B. There are two published SNPs in this region …

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pXN21-Dest

Plasmid

#105086

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Publication

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hygro(+)-PSTCD-cGAS

Plasmid

#102605

Purpose

Mammalian expression of cGAS fused to PSTCD tag

Depositor

Nicolas Manel

Publication

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

cGAS (CGAS Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-mRuby2

Plasmid

#104058

Purpose

AAV expression of Cre-dependent reporter mRuby2

Depositor

Viviana Gradinaru

Publication

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

mRuby2

Use

AAV

Promoter

CAG

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-mFgf21pro1-luc

Plasmid

#101797

Purpose

Reporter for mouse Fgf21 promoter activity

Depositor

Seiichi Oyadomari

Publication

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Fibroblast growth factor 21 promoter (Fgf21 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pNat-AID*-9myc

Plasmid

#99523

Purpose

C-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae

Depositor

Helle Ulrich

Publication

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS2+ H2B-mCherry

Plasmid

#99265

Purpose

Contains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

Depositor

Philipp Keller

Publication

Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry

Tags

H2B

Expression

Mammalian

Promoter

SP6

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-GBP-mCherry-kanMX6

Plasmid

#89068

Purpose

genomic GBP-mCherry epitope C-terminal tagging in S. pombe

Depositor

Quanwen Jin

Publication

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

GBP-mCherry

Expression

Yeast

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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ITPKA-mScarlet-I

Plasmid

#98829

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ITPKA

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti-sgp53/Cre

Plasmid

#89646

Purpose

Expresses a p53-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Publication

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting p53 (Trp53 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMonAID_nCas9-PmCDA_Hyg_ALS

Plasmid

#91693

Purpose

Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

Depositor

Akihiko Kondo

Publication

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

2x35S

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-PANX1(HCVp)

Plasmid

#87698

Purpose

Express HCV protease-activatible pannexin-1 in mammalian cells

Depositor

Robert Campbell

Publication

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PANX(HCVp)

Expression

Mammalian

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAP1_WT_V5

Plasmid

#82994

Purpose

Gateway Donor vector containing TRAP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

TRAP1 (TRAP1 Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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LOTUS-V/pcDNA3

Plasmid

#87127

Purpose

Bioluminescent and ratiometric voltage indicator

Depositor

Takeharu Nagai

Publication

Inagaki et al Sci Rep. 2017 Feb 13;7:42398. doi: 10.10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LOTUS-V

Expression

Mammalian

Promoter

CMV

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSLIK DN RhoA

Plasmid

#84646

Purpose

Lentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of Venus

Depositor

Sanjay Kumar

Publication

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

RhoA (RHOA Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Dominant Negative

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article