We narrowed to 68,612 results for: %s
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Plasmid#102879PurposeExpresses rsEGFP2 in bacterial cellsDepositorInsertrsEGFP2
Tags6xHisTagExpressionBacterialAvailable sinceJan. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-GST-4EBP1-Low GC
Plasmid#69018Purposeexpresses tagged 4EBP1DepositorInsertEIF4EBP1 (EIF4EBP1 Human)
TagsGST and HAExpressionMammalianMutationlowGC content, wt is ~70% GC rich. Still, it code…Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pETDuet RagA-RagC
Plasmid#99641PurposeBacterial expression of wildtype Rag GTPase dimerDepositorAvailable sinceDec. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
CD8-mCherry-FRB
Plasmid#100738PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD8A (CD8A Human)
TagsFRB and mCherryExpressionMammalianMutationTruncatedPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEM-Cas9HF1
Plasmid#89961PurposeModified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.DepositorInsertCas9HF1
ExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSG5-SUV39H2-FL
Plasmid#89135Purposemammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase from pSG5, includes 5' and 3' noncoding regionsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSUV39H2 (SUV39H2 Human)
ExpressionMammalianPromoterSV40Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)
Plasmid#86413Purposenuclear split GFP11 reporter with mCherryDepositorInsertNop1pr-GFP11-mCherry-PUS1
TagsGFP11 and mCherryExpressionYeastPromoterNOP1Available sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBK-YE2-BE3
Plasmid#85176PurposeExpresses YE2-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYEE-BE3
ExpressionMammalianPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-Q63L_mScarlet-i_SGFP2
Plasmid#85072PurposeGreen/red FRET biosensor for RhoA in the GTP locked constitutively active stateDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRhoA Biosensor constitutively active (Q63L) (RHOA Human)
Tags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationQ63LPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W
Plasmid#67984PurposeCas9 activity reporter with GFP and BFPDepositorInsertsU6gRNA cassette, PGKGFP2ABFP cassette, WPRE
Guide RNA targeting modified BFP
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W
Plasmid#67976PurposeCRISPR gRNA expression vector with an improved scaffold and puro/mAG markersDepositorInsertU6gRNA cassette, PGKpuro2AmAG cassette, WPRE
UseLentiviralMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBabe 3XFLAG-wtGATA6-3XAU1 puro
Plasmid#72607PurposeExpresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cellsDepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ-DY2230AA
Plasmid#64878Purposelentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant)DepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationD2330A,Y2331A, in the DNA polymerase domain (POL)…PromoterEF1alphaAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
KRas FAK biosensor
Plasmid#78301PurposeFRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKRas FAK biosensor
ExpressionMammalianPromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-RFP657
Plasmid#75162PurposeDerived from LentiCRISPR v1 but expresses RFP657 instead of puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsRFP657ExpressionMammalianAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRGD-SmR
Plasmid#74109PurposePlasmid containing streptomycin resistance gene cassette flanked by multiple restriction sitesDepositorInsertaadA
UseBacterial vector based on pmb1 repliconAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK283 (Stag-3FLAG-Neo)
Plasmid#72799PurposeC-terminal tagging with Stag-3FLAG using CRIPSR/Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStag-3FLAG-Neo
UseCRISPRTagsStag-3FLAGExpressionMammalianAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-FLAG-MTOR-V2006I
Plasmid#69012Purposeactivating MTOR mutationDepositorAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRL1383a
Plasmid#70692PurposeRSF1010-based vector with extensive polylinker, and resistance to Strep and Spec. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.DepositorTypeEmpty backboneUseBacterial shuttle vectorAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MBP-MEK1
Plasmid#68300PurposeN-terminal MBP tagged human MEK1 gene for bacterial expressionDepositorAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only