We narrowed to 10,409 results for: SEC
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Plasmid#92039PurposeExpresses mEos3-Rac1 fusion proteinDepositorAvailable SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only
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mEos3-dnRac1
Plasmid#92040PurposeExpresses mEos3 fusion with dnRac1 (T17N).DepositorInsertRAC1 (RAC1 Human)
TagsmEos3ExpressionBacterial, Insect, and Mamm…MutationT17N (dominant negative)PromoterCMVAvailable SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEos3-caRac1
Plasmid#92041PurposeExpresses mEos3 fusion with caRac1 (Q61L)DepositorInsertRAC1 (RAC1 Human)
TagsmEos3ExpressionBacterial, Insect, and Mamm…MutationQ61L (constitutively active)PromoterCMVAvailable SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin-FLEX-soCoChR-GFP
Plasmid#107712PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virusDepositorHas ServiceAAV9InsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianPromoterhSynapsinAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9-pAc
Plasmid#162163PurposeExpresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectableDepositorInsertshSpCas9
sgRNA
UseCRISPRTagsT2A-pAcExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBVboostFGII WPRE 2A-CVB3
Plasmid#202076PurposeBacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.DepositorInsertCVB3 2A protease with HisTag
Tags6xHis-tagExpressionBacterial, Insect, and Mamm…Promoterpolh, CAG, T7Available SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJM483
Plasmid#52987PurposeA secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensorDepositorInsertLactaherin (Mfge8 Mouse)
TagsGFP (w/ introns) and signal sequenceExpressionWormMutationdeleted native signal sequence, deleted C-termina…Promoterhsp-16.41Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG
Plasmid#222854PurposeMammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 FcDepositorInsertAT118-L
TagsIgG1_FcExpressionMammalianMutationL234A, L235A, P329GAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-vCre
Plasmid#55638PurposeEF1a-driven expression of VCre in Mammalian CellsDepositorHas ServiceAAV Retrograde and AAV8InsertvCre
UseAAVExpressionMammalianPromoterEf1aAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-EYFP
Plasmid#231927PurposeCre-off/Flp-on EYFP under the Ef1a promoterDepositorInserteYFP with introns
UseAAVPromoterEf1aAvailable SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10
Plasmid#218863PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8m
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationA25G F286YPromoterhsp70Available SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Coff/FonEYFP
Plasmid#55652PurposeCre-off/Flp-on EYFP under the Synapsin promoterDepositorInserteYFP with introns
UseAAV; Cre off/flp on eyfpExpressionMammalianPromoterhSynAvailable SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
GANLS
Plasmid#50852PurposeExpresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.DepositorInsertddGFP A
Tagstriplicated NLS sequence DPKKKRKVExpressionMammalianPromoterCMVAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
RANLS
Plasmid#50843PurposeExpresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.DepositorInsertddRFP A
Tagstriplicated NLS sequence DPKKKRKVExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
IR25a-T2A-QF-AD
Plasmid#162526PurposePlasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a geneDepositorInsertIr25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
ExpressionInsectAvailable SinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1
Plasmid#162164PurposeSelectable homology donor template for Aag2 cell AGO1DepositorInsertsRFP
3xFLAG
UseCRISPR and Cre/Lox; Homology donor templateTagsAGO1 homology and loxPExpressionInsectPromoterAe. aegypti PUb and endogenous AGO1Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRVdGL-4Flpo
Plasmid#98040PurposeSecond-generation rabies viral vector genomeDepositorInsertFlpo
ExpressionMammalianPromoterCMVAvailable SinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
ORCO-T2A-QF-DBD
Plasmid#162525PurposePlasmid for CRISPR-mediated knock-in line that expresses the DNA-binding domain of QF by knocking-in a T2A-QF-DBD fragment replacing the stop codon of the endogenous Aedes aegypti Orco geneDepositorInsertOrco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
ExpressionInsectAvailable SinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAW-NbVHH05-mCherry
Plasmid#171572PurposeExpresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cellsDepositorAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(S473D)
Plasmid#86577PurposeExpresses cleavable human full-length Akt1(S473D) phosphomimeticDepositorInsertAkt1 (AKT1 Human)
Tags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130; S473D phos…Available SinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only