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4,203 results

Showing: 821 - 840 of 4203 results

  3. pLV-TetO-hNGN2-eGFP-Puro

    Plasmid
    #79823
    Purpose
    Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.
    Depositor
    Kristen Brennand
    Article
    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15
    Insert
    hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
    Use
    Lentiviral
    Promoter
    TetO
    Available Since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79823-plasmid-map-sequence-id-240610

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  7. pCW57.1-FOXM1b

    Plasmid
    #68811
    Purpose
    Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells
    Depositor
    Adam Karpf
    Article
    Barger et al Oncotarget. 2015 Jul 16.
    Insert
    FOXM1b (FOXM1 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    HA
    Expression
    Mammalian
    Promoter
    TRE promoter, Tet ON
    Available Since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    68811-plasmid-map-sequence-id-419548

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  8. pBOB-CAG-SARS-CoV2-Spike-HA

    Plasmid
    #141347
    Purpose
    3rd Generation lentiviral vector expressing the codon-optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao
    Depositor
    Gerald Pao
    Article
    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.
    Insert
    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    Use
    Lentiviral
    Tags
    HA
    Mutation
    resynthesized with human codon optimization nucle…
    Promoter
    CAG
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    141347-plasmid-map-sequence-id-296391

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  16. lenti-sgRNA hygro

    Plasmid
    #104991
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104991-plasmid-map-sequence-id-335144

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  18. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

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Showing: 821 - 840 of 4203 results