4,168 results

1. pLX304 CMV FKBP-V5-sTurboID (N)

   Plasmid

   #153002

   Purpose

   Expresses low-affinity split-TurboID N-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (N) (L73)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-BbsI-PITCh

   Plasmid

   #127875

   Purpose

   For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

   Plasmid

   #119816

   Purpose

   3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

   Depositor

   Ethan Abel

   Article

   Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

   Inserts

   Luciferase
   Puromycin
   

   Use

   Lentiviral and Luciferase

   Tags

   EGFP and V5

   Expression

   Mammalian

   Mutation

   Stop codon removed in the frame of the V5 frame v…

   Promoter

   CMV/TO and Human Phosphoglycerate kinase

   Available Since

   Jan. 2, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pC0046-EF1a-PspCas13b-NES-HIV

   Plasmid

   #103862

   Purpose

   Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Available Since

   Nov. 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDG459

   Plasmid

   #100901

   Purpose

   SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-PuroR

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL1521 (pSPIN, pSC101* backbone)

   Plasmid

   #160729

   Purpose

   Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti sgRNA(MS2)_zeo backbone

   Plasmid

   #61427

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRIS-PITChv2-FBL

   Plasmid

   #63672

   Purpose

   PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   EGFP-2A-PuroR
   

   Use

   CRISPR

   Promoter

   Promoterless

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFPC1-huNFATc1EE-WT

   Plasmid

   #24219

   Depositor

   Jerry Crabtree

   Article

   Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

   Insert

   NFATc1 (NFATC1 Human)
   

   Tags

   EE and GFP

   Expression

   Mammalian

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. PPRE X3-TK-luc

    Plasmid

    #1015

    Purpose

    PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

    Depositor

    Bruce Spiegelman

    Article

    Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

    Insert

    PPRE
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Nov. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATeam1.03-nD/nA/pcDNA3

    Plasmid

    #51958

    Purpose

    mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

    Insert

    ATeam1.03-nD/nA
    

    Expression

    Mammalian

    Available Since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. LSL-Cas9-Rosa26TV

    Plasmid

    #61408

    Purpose

    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    Cas9
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    3xFLAG and P2A

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CAG

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD33.1

    Plasmid

    #36267

    Depositor

    Christian Raetz

    Article

    Chung et al Biochemistry. 2010 May 18;49(19):4126-37

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSFFV_sfCherry3C(1-10)

    Plasmid

    #117482

    Purpose

    Lentiviral vector for constitutive expression of sfCherry3C(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    sfCherry3C(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    5 substitutions (K45R, G52D, T106A, K182R, N194D)…

    Promoter

    SFFV

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHCMV-EcoEnv

    Plasmid

    #15802

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    MLV env (ecotropic)
    

    Expression

    Mammalian

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPRv2 puro

    Plasmid

    #98290

    Purpose

    Variant of lentiCRISPRv2 that confers puromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF-1a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAdTrack-CMV

    Plasmid

    #16405

    Purpose

    Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Adenoviral

    Expression

    Mammalian

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-2xFYVE

    Plasmid

    #140047

    Purpose

    Expresses GFP-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available Since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mito-BFP

    Plasmid

    #49151

    Purpose

    visualization of mitochondria

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    mitochondrial targeting signal (COX4 Budding Yeast)
    

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti CMV/TO SV40 small + Large T (w612-1)

    Plasmid

    #22298

    Depositor

    Eric Campeau

    Insert

    SV40 small + Large T antigen
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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