4,233 results

Showing: 821 - 840 of 4233 results

Results per page:

Sort results by:

mCherry-Mito-7

Plasmid

#55102

Purpose

Localization: Mitochondria, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

Insert

Mito (COX8A Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBP-LbCas12a

Plasmid

#113431

Purpose

Expresses 10xHis-MBP fused to LbCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Has Service

Cloning Grade DNA

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYL156 (TRV RNA2)

Plasmid

#148969

Purpose

Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

Depositor

Savithramma Dinesh-Kumar

Article

Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

Insert

Modified TRV RNA2

Use

T-dna vector

Mutation

see comments section below

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-GST

Plasmid

#42049

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

GST

Tags

His

Expression

Bacterial

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pRGEB32

Plasmid

#63142

Purpose

Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

Depositor

Yinong Yang

Article

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Rice snoRNA U3 promoter for gRNA/PTG expression a…

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK381 (AAVS1 CMV-OsTIR1F74G)

Plasmid

#140536

Purpose

AAVS1 CMV-OsTIR1(F74G)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CMV-OsTIR1(F74G)

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MP6

Plasmid

#69669

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR ugi CDA1

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPRv2 hygro

Plasmid

#98291

Purpose

Variant of lentiCRISPRv2 that confers hygromycin resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
YAP/TAZ-TRE-mStrawberry reporter

Plasmid

#158682

Purpose

This plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDx_mScarlet-I3

Plasmid

#189757

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMV-AmphoEnv

Plasmid

#15799

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

4070A Amphotropic Murine leukemia virus envelope gene

Expression

Mammalian

Mutation

N/A

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyCatcher003 S49C

Plasmid

#133448

Purpose

Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003 S49C

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Contains S49C mutation

Promoter

T7

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-mitoDsRed

Plasmid

#44386

Purpose

3rd generation lentiviral vector

Depositor

Pantelis Tsoulfas

Article

Kitay et al Hum Mol Genet. 2013 Jan 24.

Insert

mitoDsRed

Use

Lentiviral

Tags

61 aa targeting sequence of the P1 isoform F1F0-A…

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Tet-pLKO-puro-Scrambled

Plasmid

#47541

Purpose

Tet inducible scrambled shRNA.

Depositor

Charles Rudin

Article

Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

Insert

Scrambled shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

Plasmid

#67974

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2ABFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PH-PLCD1-GFP

Plasmid

#51407

Purpose

Is a biosensor for PI(4,5)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

PH-PLCD1 (aa 1–170) (PLCD1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDG459

Plasmid

#100901

Purpose

SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

Depositor

Paul Thomas

Article

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG and T2A-PuroR

Expression

Mammalian

Promoter

CBh and U6

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PEmax-P2A-hMLH1dn

Plasmid

#174828

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-hMLH1dn

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript; deletion of residues 754-…

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiFC-VC155

Plasmid

#22011

Purpose

Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

VC155

Tags

HA

Expression

Mammalian

Mutation

Changed Alanine 206 to Lysine

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyCatcher003-sfGFP

Plasmid

#133449

Purpose

Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003-sfGFP

Tags

His6 and TEV site

Expression

Bacterial

Promoter

T5

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge