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Showing: 1231 - 1260 of 17337 results

  1. pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry

    Plasmid
    #226104
    Purpose
    Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    COX8A (COX8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    encodes only for mitochondrial targeting sequence…
    Promoter
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    226104-plasmid-map-sequence-id-448409

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  2. pTwist-SARS-CoV-2 Spike-deltaC18 (P.1) - No RBD Mutations

    Plasmid
    #212384
    Purpose
    Encodes SARS-CoV-2 spike L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V1176F for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V1176F (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V11…
    Promoter
    CAG
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212384-plasmid-map-sequence-id-431624

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  3. L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

    Plasmid
    #222876
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FRB chain: V84R in GPA
    Promoter
    MSCV LTR
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222876-plasmid-map-sequence-id-441621

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  4. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

    Plasmid
    #218973
    Purpose
    Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutant SRSF1 binding motif (AGTCTCCT)
    Promoter
    RSV LTR
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218973-plasmid-map-sequence-id-436111

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  9. pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut_R

    Plasmid
    #147395
    Purpose
    Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut
    Depositor
    Elisa Izaurralde
    Article
    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94
    Insert
    CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut (TNRC6C Human, Nematode)
    Use
    Tags
    Expression
    Insect
    Mutation
    Deletion of 2 AA (SD) at position 378-379 compare…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147395-plasmid-map-sequence-id-289842

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  11. pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut_R

    Plasmid
    #147399
    Purpose
    Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut
    Depositor
    Elisa Izaurralde
    Article
    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94
    Insert
    CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut (TNRC6C Human, Nematode)
    Use
    Tags
    Expression
    Insect
    Mutation
    Deletion of 2 AA (SD) at position 378-379 compare…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147399-plasmid-map-sequence-id-289846

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  21. pLV-CMV-EGFP-PolB(K72A)-PAMmut-Hygro

    Plasmid
    #176087
    Purpose
    EGFP fused to the N-terminus of POLB containing the mutation Lys72Ala, a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Lys72 to Ala, and mutation in the PAM…
    Promoter
    CMV
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    176087-plasmid-map-sequence-id-352865

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  23. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126758
    Purpose
    Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    evoSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126758-plasmid-map-sequence-id-412365

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  24. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126759
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    HeFSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126759-plasmid-map-sequence-id-412375

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  26. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126754
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126754-plasmid-map-sequence-id-410972

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  27. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126756
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    HypaSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N692A, M694A, Q695A, H698A
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126756-plasmid-map-sequence-id-410579

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  30. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

    Plasmid
    #62580
    Purpose
    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.
    Depositor
    Michael Ruppert
    Article
    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6
    Insert
    SPRED1 (SPRED1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    miR-21 binding site 1 mutant (TAAGCTA --> TAGA…
    Promoter
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1231 - 1260 of 17337 results