Addgene

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Showing: 1641 - 1680 of 60782 results

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pFR_CrPV_xb

Plasmid

#11509

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 15, 2006

Availability

Academic Institutions and Nonprofits only

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LRG2.1-BFP

Plasmid

#120577

Purpose

Lentiviral plasmid to co-express a guide RNA and EBFP.

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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FUtdTW

Plasmid

#22478

Depositor

Connie Cepko

Article

Rompani et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

tandem dimer tomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2+ GCamp6-3NLS

Plasmid

#165401

Purpose

Expresses a nuclear localized genetically encoded calcium reporter.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

Nuclear Localization Signal

Use

Avian, xenopus, zebrafish

Tags

GCamp6

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT miniAID-EGFP

Plasmid

#101713

Purpose

For amplification of a miniAID-EGFP for C-terminal tagging

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

miniAID-EGFP

Use

Tags

miniAID-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-Src

Plasmid

#118308

Purpose

Expression of mouse Src

Depositor

Philip Stork

Article

Schmitt et al Mol Cell. 2002 Jan;9(1):85-94.

Insert

Src (Src Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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p285 TIR1_HA_mH11

Plasmid

#164855

Purpose

constitutive TIR1 expression vector for mouse H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

OsTIR1

Use

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-Sall4

Plasmid

#13361

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

sal-like 4 (Drosophila) (Sall4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

*(see below)

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pNL-EGFP/CMV/WPREdU3

Plasmid

#17579

Depositor

Jakob Reiser

Article

Ricks et al Stem Cells Dev. 2008 Jun . 17(3):441-50.

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-miR21

Plasmid

#21114

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 9. ()

Insert

miR-21 (MIR21 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
LRG2.1-TagBFP2

Plasmid

#124773

Purpose

Lentiviral plasmid to co-express a guide RNA and TagBFP2

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT EGFP-miniAID

Plasmid

#101714

Purpose

For amplification of a EGFP-miniAID for N-terminal tagging

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

EGFP-miniAID

Use

Tags

EGFP-miniAID

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+ EGFP-3NLS

Plasmid

#165400

Purpose

Expresses nuclear localized EGFP

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

Nuclear Localization Signal

Use

Avian, xenopus, zebrafish

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pReadthroughUGA

Plasmid

#11513

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, UAA stop codon, Renilla luciferase, and 3' UTR short RNA binding sites

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 15, 2006

Availability

Academic Institutions and Nonprofits only
pReadthroughUAA

Plasmid

#11512

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, UAA stop codon, Renilla luciferase, and 3' UTR short RNA binding sites

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 15, 2006

Availability

Academic Institutions and Nonprofits only
pReadthroughUAG

Plasmid

#11511

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, UAG stop codon, Renilla luciferase, and 3' UTR short RNA binding sites

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 15, 2006

Availability

Academic Institutions and Nonprofits only
pAA173

Plasmid

#21743

Depositor

David Sherwood

Article

Ziel et al Nat Cell Biol. 2009 Feb . 11(2):183-9.

Insert

pie-1>mCherry::PH domain (PLC-delta)

Use

Bacterial propagation

Tags

mCherry

Expression

Mutation

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pLTE EGFP

Plasmid

#67044

Purpose

Cell-free expression control

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

EGFP

Use

Cell-free expression

Tags

Myc

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pOZ-FH-C-puro

Plasmid

#32516

Depositor

Andrew Lassar

Article

Kumar et al Mol Biol Cell. 2009 Jul;20(14):3170-7. E

Type

Empty backbone

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only
pJWZ6

Plasmid

#21744

Depositor

David Sherwood

Article

Ziel et al Nat Cell Biol. 2009 Feb . 11(2):183-9.

Insert

mCherry::dMoeABD

Use

Bacterial propagation

Tags

mCherry

Expression

Mutation

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
sZF15

Plasmid

#43846

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF15

Use

Tags

HA Flag and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pET-UbcH10-wt

Plasmid

#8503

Depositor

Joan Ruderman

Article

Bastians et al Mol Biol Cell 1999 Nov;10(11):3927-41.

Insert

UbcH10 (UBE2C Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pMXs-Sox15

Plasmid

#13359

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

SRY-box containing gene 15 (Sox15 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pMPRA2

Plasmid

#49350

Purpose

Backbone for transient transfection of Massively Parallel Reporter Assays (MPRA) libraries, pPGK-H2Kk for physical selection of transfected cells

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
NK644

Plasmid

#109096

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_00306 geranyl-geranylation signal

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pT/SVNeo

Plasmid

#24550

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

pT/SVNeo

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only
ZS007:eGFP-TM

Plasmid

#186429

Purpose

eGFP-TM

Depositor

Adam Cohen

Article

Shi et al Cell. 2018 Dec 13;175(7):1769-1779.e13.

Insert

eGFP-TM

Use

Tags

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
sZF12

Plasmid

#43843

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF12

Use

Tags

HA Flag and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
NK612

Plasmid

#109092

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

Lifeact-mCherry

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TSC2-S1345A

Plasmid

#29358

Depositor

Kunliang Guan

Article

Inoki et al Cell. 2003 Nov 26. 115(5):577-90.

Insert

TSC2 (Tsc2 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

S1345A (equivalent to S1389A in rat TSC2 isoform …

Promoter

Available since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
sZF2

Plasmid

#43833

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF2

Use

Tags

HA Flag and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pGL3con-miR15aPer

Plasmid

#21116

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 9. ()

Insert

hsa-miR-15a sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
sZF1

Plasmid

#43832

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF1

Use

Tags

HA Flag and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
sZF3

Plasmid

#43834

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF3

Use

Tags

HA Flag and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
TLD_009

Plasmid

#19695

Depositor

James Collins

Article

Deans et al Cell. 2007 Jul 27. 130(2):363-72.

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pET His10-Src42a

Plasmid

#126674

Purpose

Expression of His10-Src42a for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Src42a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b/Shigella flexneri IpaA

Plasmid

#46178

Depositor

Susan Craig

Article

Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

Insert

IpaA

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
NK641

Plasmid

#109100

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_07215

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NK601

Plasmid

#109070

Purpose

Parental vector for making S. rosetta expression vectors

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_03784

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
cmv-sfGFP-GB1-scAB

Plasmid

#185794

Purpose

scFv GFP compnent for SunRISER

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

sfGFP-GB1-scAB

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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