We narrowed to 17,356 results for: URE

1. pAd 2B Ngn3-I-nGFP

   Plasmid

   #19410

   Depositor

   Douglas Melton

   Article

   Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

   Insert

   neurogenin 3 (Neurog3 Mouse)
   

   Use

   Adenoviral; Entry vector

   Tags

   GFP and IRES

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pXylA-agrCA-I

   Plasmid

   #53437

   Purpose

   xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   agrCA locus
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial

   Mutation

   "MVQTS" sequence added to the N-terminu…

   Promoter

   PxylA

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HBT-GCaMP6-HA

   Plasmid

   #107433

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   GCaMP6S
   

   Use

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. His6-Twin-Strep-SUMO-pET28a-EfaCas2

   Plasmid

   #112794

   Purpose

   To express enterococcus faecalis Cas2 protein in E. coli

   Depositor

   Ailong Ke

   Article

   Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

   Insert

   EfaCas2
   

   Use

   Tags

   His6-Twin-Strep-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. untargeted Plk1 FRET sensor Myt1 substrate

   Plasmid

   #45217

   Depositor

   Michael Lampson

   Article

   Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

   Insert

   untargeted Plk1 FRET sensor Myt1 substrate
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT7C-HSPA1L

   Plasmid

   #177660

   Purpose

   Recombinant protein synthesis of human HSPA1L

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   HSPA1L (HSPA1L Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSMP-YY1_1

   Plasmid

   #36357

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   YY1 (YY1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-FLAG-mTUT5

   Plasmid

   #60042

   Purpose

   Mammalian expression of FLAG-tagged mouse TUT5

   Depositor

   Zissimos Mourelatos

   Article

   Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

   Insert

   TUT5 (Papd7 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

9. CENP-B targeted Aurora B FRET sensor Kif2 substrate

   Plasmid

   #45214

   Depositor

   Michael Lampson

   Article

   Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

   Insert

   CENP-B targeted Aurora B FRET sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available Since

   May 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ki67∆repeats-mNeonGreen-IRESpuro2

    Plasmid

    #183742

    Purpose

    Expresses Ki67∆repeats-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67(lacking repeats) (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67(lacking repeats)

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CAGhP14

    Plasmid

    #84362

    Purpose

    express FLAG-HA tagged human PRDM14

    Depositor

    Danny Reinberg

    Article

    Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    silent mutations at coding silent 1068CtoT, 1629T…

    Promoter

    CAG

    Available Since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pAB094e

    Plasmid

    #79245

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PBAD rpoZ-HA4 (Y87A); PC araC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD; PC

    Available Since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PROM5_35Sx2

    Plasmid

    #136112

    Purpose

    Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM_35Sx2 (CaMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. PROM5_35S

    Plasmid

    #136111

    Purpose

    Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM_35S (CaMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pSMP-Suv39H1_1

    Plasmid

    #36342

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Suv39H1 (SUV39H1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pAB045a

    Plasmid

    #79224

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ rpoZ-HA4; PlacIq lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ; PlacIq

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L1_KanR-Ck1

    Plasmid

    #136123

    Purpose

    L1 in position 1 (next to LB in L2 construct), for KanR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpEF1a:nptII_3t35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCk1_spacer

    Plasmid

    #136071

    Purpose

    Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pAB035h

    Plasmid

    #79191

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Plac62 gIII, luxAB; Ptet rpoZ-HA4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac62; Ptet

    Available Since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSMP-YY1_2

    Plasmid

    #36358

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    YY1 (YY1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pET30 GLTP

    Plasmid

    #170733

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

    Insert

    GLTP (GLTP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAWH

    Plasmid

    #133894

    Purpose

    Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBKWH

    Plasmid

    #133898

    Purpose

    Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDDGFP-Leu2d-ScVrg4

    Plasmid

    #102334

    Purpose

    Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

    Insert

    YGL225W (VRG4 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMRX-IP-GFP-LC3-mRuby3

    Plasmid

    #184898

    Purpose

    Stable expression of GFP-LC3-mRuby3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Okawa et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    LC3B
    

    Use

    Tags

    EGFP and mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMSCVpuro-T7-hAIM2-EGFP

    Plasmid

    #51608

    Purpose

    Express T7 and GFP-tagged hAIM2 in mammalian cells

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Retroviral

    Tags

    EGFP and T7

    Expression

    Mammalian

    Mutation

    Promoter

    MESV

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRSCgfp-hAIM2

    Plasmid

    #51666

    Purpose

    Express GFP and hAIM2 in mammalian cells

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Inserts

    AIM2 (AIM2 Human)
    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and RSV

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCI-FLAG-mTUT4

    Plasmid

    #60041

    Purpose

    Mammalian expression of FLAG-tagged mouse TUT4

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

    Insert

    TUT4 (Tut4 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pET21a-mAIM2-His6

    Plasmid

    #51536

    Purpose

    Express His6-tagged mAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    Aim2 (Aim2 Mouse)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAB082b

    Plasmid

    #79234

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ-Cry1Ac-d3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppro1

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAB061i2

    Plasmid

    #79229

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ-HA4 (Y87A); PlacIq lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppro1; PlacIq

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mCas13 Twin-SUMO

    Plasmid

    #199753

    Purpose

    Expresses codon optimized miniature mCas13 for protein expression in E.coli

    Depositor

    Magdy Mahfouz

    Article

    Mahas et al ACS Synth Biol. 2021 Oct 15;10(10):2541-

    Insert

    mCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    LacI

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAG306-GPD-VPH2 chr I

    Plasmid

    #41897

    Purpose

    Yeast expression of VPH2.

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Insert

    VPH2 (VPH2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available Since

    Feb. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-FLAG-mTUT7

    Plasmid

    #60044

    Purpose

    Mammalian expression of FLAG-tagged mouse TUT7

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

    Insert

    TUT7 (Zcchc6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

35. pSH1

    Plasmid

    #90295

    Purpose

    Expresses the fat-7 gene in C. elegans intestine

    Depositor

    Anne Brunet

    Article

    Han et al Nature. 2017 Apr 13;544(7649):185-190. d

    Insert

    fat-7 (fat-7 Nematode)
    

    Use

    Tags

    No

    Expression

    Worm

    Mutation

    Promoter

    ges-1

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 9 kB VEGF-luc

    Plasmid

    #27986

    Depositor

    Patricia D'Amore

    Article

    Loureiro et al Biochem Biophys Res Commun. 2005 Jan 14.

    Insert

    VEGF (Vegfa Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAB082h

    Plasmid

    #79240

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ-Cry1Ac-d23C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppro1

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAcGFP-Jpx9C104T

    Plasmid

    #120381

    Purpose

    Reporter for RNA nuclear localization

    Depositor

    Igor Ulitsky

    Article

    Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

    Insert

    JPX (JPX Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    C321T

    Promoter

    CMV

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSicoR-mCh-Oct4i

    Plasmid

    #21906

    Depositor

    Miguel Ramalho-Santos

    Article

    Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

    Insert

    Oct4i
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pT7-agrBD-I

    Plasmid

    #53439

    Purpose

    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrBD locus
    

    Use

    Synthetic Biology

    Tags

    V5 epitope, 6x HIS (not expressed on agrB or agrD…

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7-lac

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pSMP-Suv39H1_2

    Plasmid

    #36343

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Suv39H1 (SUV39H1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

42. pSTARR-seq_fly-RpS12

    Plasmid

    #71504

    Purpose

    flySTARR-seq screening vector with RpS12 core promoter

    Depositor

    Alexander Stark

    Article

    Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

    Type

    Empty backbone

    Use

    Flystarr-seq screening vector

    Tags

    sgGFP (SuperGlo, Qbiogene, Inc)

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSicoR (Puro) p65 shRNA2

    Plasmid

    #22508

    Depositor

    Tyler Jacks

    Article

    Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

    Insert

    p65 shRNA2 (Rela Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

44. pASKt15C+SrtA5

    Plasmid

    #64985

    Purpose

    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. hP14Gal4

    Plasmid

    #84364

    Purpose

    Gal4-PRDM14 funsion plasmid for luciferase recruitment assay

    Depositor

    Danny Reinberg

    Article

    Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Tags

    Gal4 and HA tag

    Expression

    Mammalian

    Mutation

    silent mutations at coding silent 1068CtoT, 1629T…

    Promoter

    CMV/TO

    Available Since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pSicoR-mCh-GFPi

    Plasmid

    #21905

    Depositor

    Miguel Ramalho-Santos

    Article

    Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

    Insert

    GFPi
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pU6nH1-HD10kb

    Plasmid

    #172657

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-HD10kbA/pegRNA-HD10kbB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. L1_UBE2:mS-Lt-Ck4

    Plasmid

    #136132

    Purpose

    L1 in position 4, mark cell outline with mScarletI

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpUBE2:mScarletI-Lti6b_3tNos-35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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49. pEGFP-N1-T7-hAIM2

    Plasmid

    #51541

    Purpose

    Express T7 and GFP-tagged hAIM2 in mammalian cells

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Tags

    GFP and T7

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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50. pASKt15C+SrtA7woH

    Plasmid

    #65020

    Purpose

    Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, E105K, E108A, D16…

    Promoter

    tetracycline promoter/operator

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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