We narrowed to 1,827 results for: pb

1. PB-EEA-5g-OSK2M2L1-PGK-Puro

   Plasmid

   #102909

   Purpose

   PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-SKII-URA-GAL1-fSNAP-3xHA

   Plasmid

   #188935

   Purpose

   Integration cassette to create N-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   URA-GAL1-fSNAP-3xHA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-TarHom-Tol2-kanaR-cryaa-dsRed

   Plasmid

   #74152

   Purpose

   BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker dsRed

   Depositor

   Holger Knaut

   Article

   Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

   Insert

   Tol2-kanaR-cryaa-dsRed
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS NL4-3 envFS eGFP IN-D116A

   Plasmid

   #101333

   Purpose

   pBS NL4-3 envFS eGFP with mutation that disrupts IN catalytic activity

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3 envFS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Integrase D116A

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYJ34-PB-CRISPR-BANCR-E1-gRNA

   Plasmid

   #131077

   Purpose

   long non-coding RNA BANCR knock out

   Depositor

   Xiaojun Lian

   Article

   Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

   Insert

   BANCR Exon1 gRNA (BANCR Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS NL4-3 envFS eGFP Δrev

   Plasmid

   #101348

   Purpose

   All mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   delta rev

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-EF1-IRES-NEO mutant ITR

   Plasmid

   #67905

   Purpose

   PiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutation

   Depositor

   Alex Kentsis

   Article

   Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

   Insert

   NeoR (neoR Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-Ef1a-RPS27A-UTR-PP7-WPRE

   Plasmid

   #198339

   Purpose

   PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

   Depositor

   Michael Ward

   Article

   De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

   Insert

   RPS27A-3'UTR-PP7 stem loops x24
   

   Use

   Piggybac

   Tags

   PP7 stem loops

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-SKII-URA-GAL1-fCLIP-3xHA

   Plasmid

   #188936

   Purpose

   Integration cassette to create N-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   URA-GAL1-fCLIP-3xHA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

    Plasmid

    #175506

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-TarHom-Tol2-kanaR-cryaa-sfGFP

    Plasmid

    #74153

    Purpose

    BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker sfGFP

    Depositor

    Holger Knaut

    Article

    Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

    Insert

    Tol2-kanaR-cryaa-sfGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-Ef1a-SunTag-RPS7-PP7-WPRE

    Plasmid

    #198338

    Purpose

    PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    GCN4x24-RPS7-3'UTR-PP7 stem loops x24
    

    Use

    Piggybac

    Tags

    PP7 stem loops and SunTag

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-I-Sce1-slc6a3[11kb]:egfp

    Plasmid

    #53240

    Purpose

    allow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthood

    Depositor

    Edward Burton

    Article

    Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

    Insert

    DAT-GFP
    

    Use

    Cloning

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB-6HIS-TEV-mEGFP,IRES-NGFR

    Plasmid

    #164521

    Purpose

    All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBS-SKII-URA-GAL1-HALO-GS

    Plasmid

    #188940

    Purpose

    Integration cassette to create N-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    pBS-SKII-URA-GAL1-HALO-GS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-IndHom-Tol2-TetR-cryaa-dsRed

    Plasmid

    #73204

    Purpose

    BAC targeting cassette for pIndigoBAC-536 with tetR selection marker and lens-specific transgenesis marker dsRed

    Depositor

    Holger Knaut

    Article

    Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

    Insert

    dsRed
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-TarHom-Tol2-kanaR-cryaa-YFP

    Plasmid

    #74154

    Purpose

    BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Citrine

    Depositor

    Holger Knaut

    Article

    Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

    Insert

    Tol2-kanaR-cryaa-YFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS-MCS1M(8)Kana-mCMV-pA

    Plasmid

    #22059

    Depositor

    Maria Castro

    Article

    King et al J Virol. 2008 May . 82(9):4680-4.

    Insert

    mCMV-pA expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pBS/U6/RNAi SadBs shRNA mouse

    Plasmid

    #67152

    Purpose

    pBS/U6 containing shRNA to mouse SADBs

    Depositor

    Ana Carrera, Maria Alvarado-Kristensson

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    BRSK1 (Brsk1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-Haunt E1-PuroT2AmCherry-4A KI

    Plasmid

    #92164

    Purpose

    For lncRNA 4_pA(3_SV40 polyA and 1 BGH polyA) homologue recombination mediated knockin

    Depositor

    Xiaohua Shen

    Article

    Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

    Insert

    Haunt P-Inr2-cDNA KI homology arms
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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