We narrowed to 16,583 results for: EMB

1. mRFP-LAP2β

   Plasmid

   #228029

   Purpose

   lentiviral construct for mRFP-LAP2β expression

   Depositor

   David Pellman

   Article

   Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

   Insert

   LAP2b (Tmpo Rat)
   

   Use

   Lentiviral

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNeonGreen-C1-Nir2-LNS2

   Plasmid

   #220086

   Purpose

   C-terminal PA binding domain of Nir2

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   PITPNM1 (PITPNM1 Human)
   

   Use

   Tags

   NeonGreen-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 896-1216

   Promoter

   CMV

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTK88_GFP-Anillin

   Plasmid

   #46354

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   Anillin (ANLN Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   a1854 is changed to c to disrupt XhoI site withou…

   Promoter

   pBABE

   Available since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-CAG_3C-Twin-Strep

   Plasmid

   #113901

   Purpose

   Mammalian protein expression

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiGuide-RRBP1-1

   Plasmid

   #92156

   Purpose

   CRISPR guide RNA targeting human RRBP1

   Depositor

   Alice Ting

   Article

   Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

   Insert

   RRBP1 sgRNA-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pALOD4 non-binding mutant

   Plasmid

   #111027

   Purpose

   Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

   Depositor

   Arun Radhakrishnan

   Article

   Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

   Insert

   Domain 4 of Anthrolysin O
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: S404C, C472A, G501A, T502A, T503A, L5…

   Promoter

   T7

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Cas13d-T2A-Citrine

   Plasmid

   #224565

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   Cas13d-T2A-Citrine
   

   Use

   CRISPR

   Tags

   T2A-Citrine

   Expression

   Mutation

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-CD81-(TEV)-Cre

   Plasmid

   #167939

   Purpose

   human CD81-TEV cleavage site-Cre

   Depositor

   Masaharu Somiya

   Article

   Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

   Insert

   human CD81 (CD81 Human)
   

   Use

   Tags

   Cre recombinase and TEV protease cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPerimCh

   Plasmid

   #227013

   Purpose

   Expresses periplasmic mCherry

   Depositor

   Suzan Rooijakkers

   Article

   Heesterbeek et al Sci Rep. 2019 Feb 28;9(1):3074. doi: 10.

   Insert

   PelB-mCherry, arabinose inducible cytosolic GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsM and pBAD

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA5_FRT_TO_TurboID-mCherry-Cry2

    Plasmid

    #166504

    Purpose

    Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.

    Depositor

    Angelos Constantinou

    Article

    Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2

    Insert

    None
    

    Use

    Tags

    TurboID and mCherry-CRY2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry-C1-Nir1-LNS2

    Plasmid

    #220084

    Purpose

    high affinity PA biosensor with red fluorophore

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Use

    Tags

    mCherry-GGSGGM

    Expression

    Mammalian

    Mutation

    amino acids 613-897

    Promoter

    CMV

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3/Myc-DNMT3A2

    Plasmid

    #36941

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3A2 (DNMT3A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWPI/hPLK2K111M/Neo

    Plasmid

    #35386

    Depositor

    Anurag Tandon

    Article

    Visanji et al J Biol Chem. 2011 Oct 14;286(41):35863-7

    Insert

    hPLK2 K111M (PLK2 Human)
    

    Use

    Lentiviral

    Tags

    EMCV IRES-Neomycin cassette and V5 epitope and Po…

    Expression

    Mammalian

    Mutation

    K111M

    Promoter

    EF1-alpha

    Available since

    April 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET19b-EcMscL-TEV-mEGFP

    Plasmid

    #165097

    Purpose

    Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

    Depositor

    Neha Kamat

    Article

    Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

    Insert

    E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
    

    Use

    Synthetic Biology

    Tags

    monomeric Enhanced Green Fluorescent Protein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-PH1(WT)

    Plasmid

    #86599

    Purpose

    Expresses eGFP-tagged PH(WT) domain of human Akt1

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 PH domain (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHRi_1G4 hTCRα

    Plasmid

    #187603

    Purpose

    Lentiviral expression of hTCR alpha (1G4) for low expression

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    1G4 hTCR alpha (TRA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mHSP

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Jag1-HA pIRES

    Plasmid

    #17336

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Koutelou et al J Biol Chem. 2007 Dec 12. ():.

    Insert

    Jagged1 (Jag1 Rat)
    

    Use

    Tags

    HA X3

    Expression

    Mammalian

    Mutation

    A silent C->G mutation at 3237

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-OptoPB

    Plasmid

    #113935

    Purpose

    Express a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactions

    Depositor

    Yubin Zhou

    Article

    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

    Insert

    OptoPB
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-Dcp1b

    Plasmid

    #153976

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1b

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA recapping enzybe 1b (DCP1B Human)
    

    Use

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-Tight-Puro_Nkx2.2

    Plasmid

    #64840

    Purpose

    A lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Nkx2.2 (Nkx2-2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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