We narrowed to 15,617 results for: nol
-
Plasmid#160499PurposeFor the expression of human P2X3 with ALFA tag at C-terminus in mammalian cellsDepositorInserthuman P2X3 (P2RX3 Human, Synthetic)
TagsHRV3C protease cleavage site-ALFA tag-twin Strep …ExpressionMammalianMutationΔN5ΔC33-T13P/S15V/V16IAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM4941 (pCV0053)
Plasmid#120083PurposeCyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 3 (NS3).DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS3-RK2BOM, CV-aacC1, CV-ccdB-SwaI
UseOtherAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-iGECI
Plasmid#160423PurposeAAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECIDepositorInsertiGECI
UseAAVExpressionMammalianPromoterCaMKIIAvailable SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST
Plasmid#127321PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)DepositorInsertnanoLuciferase
Tags3xFLAG and CL1/PESTExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor
Plasmid#184952PurposeDonor for both PiggyBac and Cp36 RecombinaseDepositorInsertPuroR, P2A, mTagBFP2
ExpressionMammalianAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.PAC
Plasmid#57829PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC
UseCRISPR and LentiviralTagsFLAGAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
Circular 100,50 (RAB7A)
Plasmid#170117PurposeAAV vector carrying a guide RNA targeting the human RAB7A mRNADepositorInsertCircular 100,50 guide RNA
UseAAVExpressionMammalianPromoterHuman U6Available SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
AA179
Plasmid#215939PurposeFragmid fragment: (guide cassette) short sequence with no known functionDepositorHas ServiceCloning Grade DNAInsertFiller
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3*HA-Palmd
Plasmid#216833Purposeexpressing HA tag and the coding sequence of mouse PalmdDepositorAvailable SinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cas12f-GE ver4.0
Plasmid#176543PurposeExpresses Cas12f-GE in mammalian cellsDepositorInsertsCas12f-GE ver4.0
Cas12f-GE ver4.0
ExpressionMammalianPromoterU6 and chicken β-actinAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbE1k-3
Plasmid#71722Purposetyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from humanDepositorPromoterpTrcAvailable SinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)
Plasmid#225433PurposeTranscriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.DepositorInsert4xCBS:mDFR:Rep/RepA:Tnos
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Circular 200,100 with interspersed loops (SMAD4)
Plasmid#180191PurposeAAV vector carrying a guide RNA targeting the human SMAD4 mRNADepositorInsertCircular 200,100 guide RNA with interspersed loops
UseAAVExpressionMammalianPromoterHuman U6Available SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDA_662
Plasmid#216101PurposeCRISPRi, EFS-driven dCas9-KRAB (ZNF10) (Cas only)DepositorInsertCas9 [Sp]
UseCRISPR and Lentiviral; Assembled vectorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_831
Plasmid#216100PurposeCRISPRa, EF1a-driven dCas9-VP64 (Cas only)DepositorInsertCas9 [Sp]
UseCRISPR and Lentiviral; Assembled vectorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max-CP1028
Plasmid#138160PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…PromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-myrAkt
Plasmid#49267Purposeretroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequenceDepositorInsertAkt (AKT1 Bovine)
UseRetroviralTagsIRES-EGFP and myrExpressionMammalianMutationN2D, D149E and A478G compared to AAW71957.1Available SinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat5A-SH2
Plasmid#46515DepositorInsertStat5A (STAT5A Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 582-693PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)
Plasmid#181789PurposeExpresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShTnsB, ShTniQ, ShCas12k-ShTnsC
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
SARS_COV2_ORF9B_HA_BASU
Plasmid#153981PurposeExpress Orf9b gene along with HA and BASUDepositorInsertorf9b
UseLentiviralExpressionMammalianAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only