We narrowed to 41,620 results for: Eras
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Plasmid#158103Purpose5'UTR stem-loop luciferase reporter: -30kcal/molDepositorInsert5'UTR synthetic stem-loop -30kcal/mol
UseLuciferaseExpressionMammalianPromoterPGKAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-3xNLS-OptoJNKi
Plasmid#89740PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11DepositorInsertNLS-OptoJNKi
UseFluorescent proteinTagsmCherryExpressionMammalianPromoterCMVAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_190-303
Plasmid#118822PurposeBacterial expression of hnRNPA2 truncation (residues 190 to 302).DepositorInserthnRNPA2 (HNRNPA2B1 Human)
TagsHis tag and TEVExpressionBacterialMutationcontains amino acids 190-302Available SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARF4-GFP
Plasmid#67392PurposeMammalian expression of hArf4 with C-term GFP tagDepositorInsertARF4 (ARF4 Human)
TagsGFPExpressionMammalianMutationV53A, L123P and M134I mutations were unintended P…PromoterCMVAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-GCN4-dCEN-INCENP
Plasmid#108510Purposeexpression of vsv-GCN4-dCEN-INCENPDepositorInsertGCN4-dCEN-INCENP (INCENP Human)
TagsVSVExpressionMammalianMutationreplace CEN box for GCN4 dimerazation domainPromoterCMVAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_Ptbp_ex8_3
Plasmid#155083PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKTop::tse5-CT (1169-1281)
Plasmid#192955PurposeFor membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInserttse5-CT (1169-1281)
ExpressionBacterialMutationencodes for tse5 (1169-1281)Available SinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_SFRS7_exon_deletion_3
Plasmid#155071PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_MDM4_exon_deletion_3
Plasmid#155075PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4 WW2 pos ctrl
Plasmid#112029PurposeMode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)DepositorInsertNEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
TagsSplit mCherryExpressionBacterialAvailable SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX018
Plasmid#167144PurposeMoClo-compatible Level 0-SP promoterless vector encoding Neonothopanus nambi luciferase nnLuz codon-optimised for expression in Nicotiana benthamiana and Pichia pastorisDepositorInsertfungal luciferase, nnLuz
UseLuciferase and Synthetic BiologyAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKCAG-mCherry-OptoJNKi
Plasmid#89738PurposeExpression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11DepositorInsertOptoJNKi
UseFluorescent proteinTagsmCherryExpressionMammalianPromoterCAGAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-NES-OptoJNKi
Plasmid#89739PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11DepositorInsertNLS-OptoJNKi
UseFluorescent proteinTagsmCherryExpressionMammalianPromoterCMVAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
CSK_HUMAN_D0
Plasmid#79683PurposeThis plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKCAG-H2BmCherry-OptoJNKi
Plasmid#89741PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11DepositorInsertOptoJNKi
UseOptogeneticsTagsHistone2B and mCherryExpressionMammalianPromoterCAGAvailable SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2KXIG (Tol2)-tp63:ikk1C179A:GFP
Plasmid#105647PurposeExpression of Ikk1(alpha) with mutated cysteine residues at position 179 in basal keratinocytesDepositorInserttp63:ikk1C179A:GFP (chuk Zebrafish)
TagsGFPExpressionMammalianMutationp63 promoter + ikk1C179A + GFPAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PA-mCit-NL
Plasmid#113452PurposeLentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)DepositorInsertProteinA-mCitrine-NanoLuc
UseLentiviral and LuciferaseExpressionMammalianPromoterhUBbCAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG30 IQGAP1 3'UTR mut
Plasmid#14504DepositorInsertIQGAP1 3'UTR mutant (IQGAP1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationGTGCCTT was changed to GTGCGCC and GTGCCT was cha…Available SinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -0
Plasmid#173889PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyAvailable SinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains3
Plasmid#78459PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
TagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2497-2529PromoterT7Available SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only