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Showing: 2101 - 2150 of 24948 results

  1. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196074
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196074-plasmid-map-sequence-id-389001

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  2. CBP core-dCas9-p300 core

    Plasmid
    #179558
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) & c-terminal fusion of human p300 core (aa 1048-1664) (CREBBP, EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179558-plasmid-map-sequence-id-354773

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  3. GCN5 FL-dCas9-p300 core

    Plasmid
    #179554
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179554-plasmid-map-sequence-id-354754

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  4. pCAG-eCas9-GFP-U6-gRNA

    Plasmid
    #79145
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells
    Depositor
    Jizhong Zou
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    79145-plasmid-map-sequence-id-182555

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  5. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Keith Joung
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43861-plasmid-map-sequence-id-59200

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  6. pPITX2-donor

    Plasmid
    #176372
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    PITX2 (PITX2 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176372-plasmid-map-sequence-id-347400

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  7. pCAMTA2-donor

    Plasmid
    #132501
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    CAMTA2 (CAMTA2 Human)
    Use
    CRISPR
    Tags
    GFP
    Mutation
    none
    Available Since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    132501-plasmid-map-sequence-id-274607

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  8. 3'dmDR-CMV-CasRx-bGHpolyA

    Plasmid
    #229556
    Purpose
    Expresses CasRx (RfxCas13d) with double processed direct repeats (15nt) and a non-target spacer in between at 3' end
    Depositor
    Omar Akbari
    Insert
    CasRx
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229556-plasmid-map-sequence-id-455012

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  9. pCAMTA2.1.0-gDNA

    Plasmid
    #132456
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    CAMTA2 (CAMTA2 Human)
    Use
    CRISPR
    Available Since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    132456-plasmid-map-sequence-id-266911

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  10. pMBD1.1.0-gDNA

    Plasmid
    #132444
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    MBD1 (MBD1 Human)
    Use
    CRISPR
    Available Since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132444-plasmid-map-sequence-id-266804

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  14. pCut_GFP

    Plasmid
    #240154
    Purpose
    Expresses CRISPR guides
    Depositor
    Morten Norholm
    Article
    Gude et al bioRxiv 2025.06.19.660532
    Inserts
    constant cut sites CRISPR guide array
    "self-curing" CRISPR guide array
    GFP
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Oct. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240154-plasmid-map-sequence-id-479867

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  19. pBACH2-donor

    Plasmid
    #176357
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    BACH2 (BACH2 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176357-plasmid-map-sequence-id-347870

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  21. pSOX2-donor

    Plasmid
    #176364
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    SOX2 (SOX2 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176364-plasmid-map-sequence-id-347872

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  22. px330_SMAD exon 9 gRNA

    Plasmid
    #68352
    Purpose
    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    SMAD exon 9 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68352-plasmid-map-sequence-id-119624

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  24. pID3-donor

    Plasmid
    #176342
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ID3 (ID3 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176342-plasmid-map-sequence-id-347890

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  25. pALuWBB

    Plasmid
    #183202
    Purpose
    dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA
    Depositor
    Mitch McVey
    Insert
    w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
    Use
    CRISPR; Recombines at attp landing site in fly ge…
    Expression
    Insect
    Promoter
    U6-3
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    183202-plasmid-map-sequence-id-362157

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  26. pCDX4-donor

    Plasmid
    #176343
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    CDX4 (CDX4 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176343-plasmid-map-sequence-id-347886

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  27. pJAZF1-donor

    Plasmid
    #176352
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    JAZF1 (JAZF1 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176352-plasmid-map-sequence-id-347876

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  32. pTP63.1.0-gDNA

    Plasmid
    #132432
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TP63 (TP63 Human)
    Use
    CRISPR
    Available Since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    132432-plasmid-map-sequence-id-266195

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  33. pTP63-donor

    Plasmid
    #132477
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TP63 (TP63 Human)
    Use
    CRISPR
    Tags
    GFP
    Mutation
    none
    Available Since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    132477-plasmid-map-sequence-id-273771

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  34. pE4F1.1.0-gDNA

    Plasmid
    #132451
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    E4F1 (E4F1 Human)
    Use
    CRISPR
    Available Since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    132451-plasmid-map-sequence-id-266819

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  35. pDLX4.1.0-gDNA

    Plasmid
    #132437
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    DLX4 (DLX4 Human)
    Use
    CRISPR
    Available Since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    132437-plasmid-map-sequence-id-266219

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  41. pSOX11-donor

    Plasmid
    #176360
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    SOX11 (SOX11 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176360-plasmid-map-sequence-id-347860

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  46. pELF2.1.0-gDNA

    Plasmid
    #132454
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ELF2 (ELF2 Human)
    Use
    CRISPR
    Available Since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    132454-plasmid-map-sequence-id-266832

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  47. pGRHL2-donor

    Plasmid
    #176351
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    GRHL2 (GRHL2 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176351-plasmid-map-sequence-id-347880

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  48. pCREB5-donor

    Plasmid
    #132521
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    CREB5 (CREB5 Human)
    Use
    CRISPR
    Tags
    GFP
    Mutation
    none
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132521-plasmid-map-sequence-id-273936

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  50. (915) SB EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195623
    Purpose
    Sleeping Beauty EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    EF1a (Mlu1/EcoR1)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195623-plasmid-map-sequence-id-385117

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Showing: 2101 - 2150 of 24948 results