We narrowed to 14,602 results for: pET
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Plasmid#133430PurposeYeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionBacterialMutationBoth native Cys (C59S/C252A) have been mutated. S…PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-SUMO-KaiB
Plasmid#68050PurposeStrain: AM5116, Used for protein purificationDepositorInsertKaiB
UseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-K292A
Plasmid#134575PurposeExpress human DDX43 mutant (K292A, ATPase dead) in E. coliDepositorInsertDDX43-K292A (DDX43 Human)
UseTagsHisExpressionBacterialMutationfull-length (K292A)PromoterT7Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-R399E
Plasmid#122691PurposeExpress His-tagged PKM2 (R399E) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationR399EPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
DJ-1 TEV site pET15b
Plasmid#60687PurposeRecombinant protein expression of Park7DepositorInsertDJ-1 (PARK7 Human)
UseTags6xHisExpressionBacterialMutationTEV site was inserted (see comments below).PromoterT7Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delWGR-L713F
Plasmid#173941PurposeBacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutationDepositorInsertPARP1delWGR-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 521-660; L713F, V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-27
Plasmid#172752PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-27
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Trx-PP-hNF2
Plasmid#107148Purposebacterial expressionDepositorInsertneurofibromin 2 (NF2 Human)
UseTags(His)6-ThioredoxinExpressionBacterialMutationPromoterT7Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-SUMO-KaiA
Plasmid#68049PurposeStrain: AM5115, Used for protein purificationDepositorInsertKaiA
UseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL15-His6
Plasmid#201880PurposeUsed for bacterial expression of human protein ARL15DepositorInsertARL15 (ARL15 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R49A)
Plasmid#112726PurposeArg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R49A)
UseTags6His-TEVExpressionBacterialMutationR49APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-24a-CXCR4
Plasmid#133431PurposeHuman CXCR4 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.DepositorInsertCXCR4 (CXCR4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
eGFP-Silicatein-pET28
Plasmid#198012PurposeC-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purificationDepositorInserteGFP-silicatein
UseTagshexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3a-UBE2J1 1-283
Plasmid#185348PurposeIn vitro transcription and translation in bacterial systemDepositorInsertUBE2J1 (UBE2J1 Human)
UseTagsExpressionBacterialMutationCytoplasmic domain only, aa 1-283PromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
CNNM3-CBS-YPet
Plasmid#149686PurposeFor the FRET-based binding assay between human CNNM3 CBS domain and PRL2.DepositorInsertCNNM3-CBS-YPet (CNNM3 Synthetic, Human)
UseTags8 histidine tagExpressionBacterialMutationPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaBC (pCLK1210)
Plasmid#50448PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylaseDepositorInsertPPCS PPCDC
UseTags6XHIS tagExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2
Plasmid#116953PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (72P)
Plasmid#62083PurposeExpression of human p53 (1-73) (72P) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHIsExpressionBacterialMutationcontains TAD residues 1-73; 72PPromoterT7Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-G415R
Plasmid#122693PurposeExpress His-tagged PKM2 (G415R) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationG415RPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)-SPN+IFS
Plasmid#83372PurposeExpresses SPN and IFS complex in E. coli cellDepositorInsertsSPN
IFS
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP3-His
Plasmid#67866Purposebacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tagDepositorInsertMRPP3 (KIAA0391 Human)
UseTagsHisExpressionBacterialMutationAmino acid 46 of recombinant MRPP3 is preceded by…PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET24a-AlkB deltaN11
Plasmid#73622PurposeExpresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tagDepositorInsertAlkB (alkB E. coli)
UseTagsHisExpressionBacterialMutationdeletion of first 11 AAPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pet 3d alpha 1 beta 2 CP
Plasmid#13450DepositorInsertcapping protein alpha 1 beta 2 (mouse) (Capza1 Mouse)
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET20b-GpNLuc-6xHis
Plasmid#135932PurposeExpresses the GpNLuc LumiFluor in bacterial cells.DepositorInsertGpNLuc LumiFluor
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Beclin1 (1-242)
Plasmid#24401DepositorInsertBeclin1 (BECN1 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 1-242 only.PromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pETM33_ZYMND11_coiled-coil MYND
Plasmid#178493PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertZYMND11_coiled-coil MYND (ZMYND11 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a- hPTP1e-PDZ2
Plasmid#20377DepositorInserthPTP1e PDZ2 (PTPN13 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-1xTS
Plasmid#162778PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 A141S
Plasmid#16157DepositorInsertHtrA2 (HTRA2 Human)
UseTagsHisExpressionBacterialMutationA141SPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SRAP-delCys2
Plasmid#84568PurposeEncodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2DepositorInsertSrap1 (Hmces Mouse)
UseTags6xHis and S-TagExpressionBacterialMutationdeletion of Cys2PromoterT7Available sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaR-Clux
Plasmid#47658PurposePesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)DepositorInsertPesaR-C
UseSynthetic BiologyTagsExpressionBacterialMutationAdditional esa box between -35 site and the origi…PromoterPesaR-CAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21a-mAIM2-deltaPYD
Plasmid#51535PurposeExpress His6-tagged HIN domain of mAIM2 in bacteriaDepositorInsertAim2 (Aim2 Mouse)
UseTagsHis6 and T7ExpressionBacterialMutationPromoterlac operatorAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
human TLNRD1 pET151
Plasmid#159384PurposeExpresses human TLNRD1 in bacterial cellsDepositorInsertTalin Rod Domain Containing Protein 1 (TLNRD1 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
CyPet-A206K-PRL2
Plasmid#149685PurposeFor the FRET-based binding assay between human CNNM3 CBS domain and PRL2.DepositorInsertCyPet-A206K-PRL2
UseTags8 histidine tagExpressionBacterialMutationCyPet A206KPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28 Set9 mutant
Plasmid#24083DepositorInsertSet9 (SETD7 Human)
UseTagsFLAG and HisExpressionBacterialMutationH297APromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FPPS
Plasmid#108952Purposeheterologous expression of FPPS of G. stearothermophilus in E. coliDepositorInsertFarnesyldiphosphat synthase (FPPS)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-24
Plasmid#172750PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-24
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-9
Plasmid#172742PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-9
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-HJURP-CBP
Plasmid#30393DepositorInsertHJURP CenpA Binding Domain (CENPA Budding Yeast, Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
C122-E16: CyPet
Plasmid#162896PurposeStandard Gateway entry clone encoding CyPet fluorescent proteinDepositorInsertCyPet fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
HOXD9 (human) pET
Plasmid#8570DepositorInsertHOXD9 (HOXD9 Human)
UseTagsT7ExpressionBacterialMutationPCR human cDNA clone; makes protein by in vitro T…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET His10-Src42a
Plasmid#126674PurposeExpression of His10-Src42a for purification from E. coliDepositorInsertSrc42a
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PET28-FnCas12a-EP16
Plasmid#183636PurposePET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.DepositorInsertFnCas12a-EP16
UseCRISPRTags6Ă—His TagExpressionBacterialMutationN607R, K613V, N617R, K180S, K660R, D616NPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
TrmD_HaeIn/pET28b(+) N,C-tag
Plasmid#12665DepositorInserttRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Lys13Arg
Plasmid#50726Purposeoverexpression of the human TPI1 Lys13Arg allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationchanged Lysine 13 to ArgeninePromoterT7Available sinceFeb. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Mouse 1-87)
Plasmid#62061PurposeExpression of Mouse p53 (residues 1-87) in e. coliDepositorInsertp53 (Trp53 Mouse)
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-87PromoterT7Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GST-Q11
Plasmid#185790PurposeEncodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequenceDepositorInsertGST-Q11
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-creM
Plasmid#112238PurposepET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreMDepositorInsertCreM
UseTagsMBPExpressionMutationPromoterAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-IS200
Plasmid#12604DepositorInsertIS200 transposase (SSO1474 Sulfolobus solfataricus)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-8
Plasmid#172761PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-8
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only