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pEMS1294

Plasmid

#29145

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

CAG promoter

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Oct. 20, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

Plasmid

#47869

Purpose

All-in-one plasmid targeting tdTomato

Depositor

James Thomson, Erik Sontheimer

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Inserts

NmCas9
U6pr-tracrRNA
tdTomato crRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pK18msB

Plasmid

#177839

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

Depositor

Christopher Johnson

Article

Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

Type

Empty backbone

Use

Bacterial gene replacement

Tags

Expression

Mutation

Promoter

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

Plasmid

#47868

Purpose

This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.

Depositor

James Thomson, Erik Sontheimer

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Inserts

NmCas9
U6pr-tracrRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a and U6

Available since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pJW1347

Plasmid

#154310

Purpose

Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa Linker for CT slot

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-Myc-IRES-RFP

Plasmid

#35395

Depositor

Martine Roussel

Article

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Myc (Myc Mouse)
RFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-Myc-IRES-GFP

Plasmid

#35396

Depositor

Martine Roussel

Article

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Myc (Myc Mouse)
GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
SPLINTR mCHERRY v1 backbone

Plasmid

#179778

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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SPLINTR BFP v1 backbone

Plasmid

#179779

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)

Plasmid

#47870

Purpose

All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.

Depositor

James Thomson, Erik Sontheimer

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Inserts

NmCas9
U6pr-tracrRNA
OCT4 crRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pJW1759

Plasmid

#154315

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::TEV::AID*::3xFLAG for NT slot

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1348

Plasmid

#154311

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker for NT slot

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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UCK2_Deletion_gRNA_1

Plasmid

#204672

Purpose

Dual gRNA plasmid for UCK2 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

uridine-cytidine kinase 2 (UCK2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS1172

Plasmid

#29301

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple151 (OLIG1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1353

Plasmid

#29155

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple137 (NR2E1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1352

Plasmid

#29154

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple136 (NR2E1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1264

Plasmid

#29136

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple222 (TBR1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1107

Plasmid

#29036

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple172 (RAMP3 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1265

Plasmid

#29137

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple223 (TBR1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1267

Plasmid

#29139

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple225 (TBR1 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge