We narrowed to 3,098 results for: actin

1. pPB_Cdc42-TomKat-sensor-CAAX

   Plasmid

   #172473

   Purpose

   Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.

   Depositor

   Sean R Collins

   Article

   Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

   Insert

   tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
   

   Use

   Piggybac transposon

   Tags

   polybasic and CAAX motif from Kras, tdKatushka2, …

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. EHD2-mCherry

   Plasmid

   #45933

   Purpose

   caveolar protein, confines caveolae to the plasma membrane

   Depositor

   Ari Helenius

   Article

   Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

   Insert

   Eps-15 homology domain-containing protein2 (EHD2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCB6-Cor1-HA

   Plasmid

   #102815

   Purpose

   Expression of Coronin1 in eukaryotic cells

   Depositor

   Jean Pieters

   Article

   Gatfield et al Mol Biol Cell. 2005 Jun;16(6):2786-98. E

   Insert

   TACO/Coronin 1 (Coro1a Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28b_RanWT

   Plasmid

   #186270

   Purpose

   Expression vector of His-RanQ69L in E.coli

   Depositor

   Masahito Ikawa

   Article

   Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   GTP-binding nuclear protein Ran (Ran Mouse)
   

   Use

   Tags

   His tagged

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_PP1-mCitrine

   Plasmid

   #172472

   Purpose

   Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

   Depositor

   Sean R Collins

   Article

   Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

   Insert

   prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
   

   Use

   Lentiviral

   Tags

   mCitrine and prolactin signal sequence (cleaved p…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV6M-Pak1 H83L H86L

   Plasmid

   #12211

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   PBD (p21-binding domain) mutant: H83L, H86L (see …

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-PH-SWAP70

   Plasmid

   #84582

   Purpose

   Expresses mCherry-tagged PH-domain of SWAP70 in mammalian cells, binds to PI(3,4)P2

   Depositor

   Geert van den Bogaart

   Article

   Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

   Insert

   SWAP70 residues 209-306 (SWAP70 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Pleckstrin-homology domain, residues 209-306

   Promoter

   CMV

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCAG-GFP-PQRv3-RFP

   Plasmid

   #73951

   Purpose

   Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.

   Depositor

   Brian Chen

   Article

   Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

   Inserts

   sfGFP (superfolder GFP)
   RFP
   

   Use

   Tags

   A residual PQR peptide wii be left at the C-termi…

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin and Chicken beta actin (shared…

   Available since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pCMV5-Flag-EPLIN delta N-term

   Plasmid

   #40932

   Depositor

   Elizabeth Luna, Scott Maul

   Article

   Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

   Insert

   EPLIN (LIMA1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-220

   Promoter

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pCS2+MT-hToca-1-MGD

    Plasmid

    #33033

    Depositor

    Marc Kirschner

    Article

    Ho et al Cell. 2004 Jul 23;118(2):203-16.

    Insert

    Toca-1 (FNBP1L Human)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    MGD = amino acid substitutions M383I, G384S and D…

    Promoter

    simian CMV IE94

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pGEX-6P-2_dCrk

    Plasmid

    #131146

    Purpose

    Expresses GST::dCrk (based on crk isoform RA).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFX-Lifeact-mCherry

    Plasmid

    #175822

    Purpose

    Express mCherry with actin-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with actin-targeting sequence
    

    Use

    Tags

    Lifeact (N-terminal 17 amino acids from S. Cerevi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMB

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmiR-report-hEZH2

    Plasmid

    #112543

    Purpose

    3'UTR reporter assay

    Depositor

    Shusheng Wang

    Article

    Zhou et al Mol Ther. 2014 Feb;22(2):378-389. doi: 1

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-Myo 1e

    Plasmid

    #134905

    Purpose

    Expression of rat Myo 1e (Myr 3) in mammalian cells.

    Depositor

    Martin Bähler

    Article

    Stoffler et al J Cell Biol. 1995 May;129(3):819-30. doi

    Insert

    Myo 1e (rat) with 3' UTR (Myo1e Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-POLArISact

    Plasmid

    #164970

    Purpose

    T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    POLArISact
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSG5-HA-H-Ras_wt

    Plasmid

    #39509

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS (HRAS Human)
    

    Use

    Eukaryotic

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

17. AktPH-mCherry-T2A-PHR-T2A-CIBNcaax

    Plasmid

    #173861

    Purpose

    Mammalian expression of Akt PH-mCherry-T2A-PHR-T2A-CIBNcaax (PIP3 marker and opto-control)

    Depositor

    Takao Nakata

    Article

    Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

    Insert

    AktPH-mCherry-T2A-PHR-T2A-CIBNcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFX-Lifeact-iRFP

    Plasmid

    #175823

    Purpose

    Express iRFP with actin-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    iRFP with actin-targeting sequence
    

    Use

    Tags

    Lifeact (N-terminal 17 amino acids from S. Cerevi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFX-Lifeact-Venus

    Plasmid

    #174478

    Purpose

    Express Venus with actin-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with actin-targeting sequence
    

    Use

    Tags

    Lifeact (N-terminal 17 amino acids from S. Cerevi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFP-bSV1_1-1009

    Plasmid

    #129471

    Purpose

    Mammalian expression of the N-terminus + central domain of bovine SV1

    Depositor

    Elizabeth Luna

    Article

    Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

    Insert

    Supervillin (SVIL Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains amino acids 1-1009

    Promoter

    CMV

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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