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Showing: 8741 - 8760 of 65890 results

  3. pIRES-hrGFP II-TET1_CD

    Plasmid
    #83570
    Purpose
    Expresses the catalytic domain of TET1 in mammalian cells
    Depositor
    Jean-Pierre Issa
    Publication
    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    Tags
    3X FLAG tag and hr GFP II
    Expression
    Mammalian
    Mutation
    TET1 amino acids 1-1417 deleted
    Promoter
    CMV
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    83570-plasmid-map-sequence-id-191046

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  4. GST-mDia1(FH1FH2DAD)-His

    Plasmid
    #85822
    Purpose
    contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.
    Depositor
    Antoine Jégou, Guillaume Romet-Lemonne
    Publication
    Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc
    Insert
    Protein diaphanous homolog 1 (Diaph1 Mouse)
    Tags
    8*His and GST
    Expression
    Bacterial
    Promoter
    tac
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    85822-plasmid-map-sequence-id-183287

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  7. hZMPSTE24-3FLAG_pQCXIP

    Plasmid
    #89759
    Purpose
    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.
    Depositor
    Martin Dorf
    Publication
    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127
    Insert
    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    Use
    Retroviral; Bicistronic expression: puromycin re…
    Tags
    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…
    Expression
    Mammalian
    Mutation
    Y253H in ZMPSTE24 (see depositor comments below)
    Promoter
    CMV
    Available since
    June 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89759-plasmid-map-sequence-id-180605

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  11. pcDNA-mCherry-4A/4B-CAAX

    Plasmid
    #87699
    Purpose
    Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells
    Depositor
    Robert Campbell
    Publication
    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-4A/4B-CAAX
    Expression
    Mammalian
    Available since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    87699-plasmid-map-sequence-id-177046

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  13. pSLI-2xFKBP-GFP(1463000)

    Plasmid
    #85789
    Purpose
    P. falciparum integration plasmid to fuse endogenous genes with tags using SLI
    Depositor
    Tobias Spielmann
    Publication
    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R
    Use
    P. falciparum integration
    Tags
    2xFKBP, GFP
    Promoter
    none
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    85789-plasmid-map-sequence-id-166704

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  17. pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

    Plasmid
    #84246
    Purpose
    Expresses direct fusion VPR-Sa dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    VPR-tagBFP-Sa dCas9
    Puro
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    84246-plasmid-map-sequence-id-160889

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  18. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

    Plasmid
    #84243
    Purpose
    Expresses ABA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABI-tagBFP-Sa dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    84243-plasmid-map-sequence-id-160884

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  19. pET32a-DEST

    Plasmid
    #84652
    Purpose
    E. coli expression vector derived from pET32 with Gateway cassette.
    Depositor
    Neil Olszewski
    Type
    Empty backbone
    Tags
    His, S tag, and Trx Tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    84652-plasmid-map-sequence-id-162124

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  20. pTol2p2A--3mnx1:mTagBFP-NS-MCS

    Plasmid
    #78250
    Purpose
    Can be used to subclone in gene of interest into blue motor neuron expression vector.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    -3mnx1 (mnx1 Zebrafish)
    -mTagBFP-NS-MCS
    Use
    Zebrafish motor neuron expression
    Promoter
    -3mnx1
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    78250-plasmid-map-sequence-id-149784

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Showing: 8741 - 8760 of 65890 results