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CMV-CytERM-mCherry-(F30-2xPepper)10

Plasmid

#129403

Purpose

CytERM-mCherry mRNA reporter tagged with (F30-2xPepper)10

Depositor

Samie Jaffrey

Publication

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CytERM-mCherry-(F30-2xPepper)10

Expression

Mammalian

Promoter

CMV

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA6-N-3XFLAG-Gsk3b

Plasmid

#123592

Depositor

Bruce Beutler

Publication

Choi et al Science. 2019 May 10;364(6440). pii: 364

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

glycogen synthase kinase 3 beta (Gsk3b Mouse)

Tags

FLAG

Expression

Mammalian

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TC374

Plasmid

#122614

Purpose

Prpl-28::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Depositor

Tian Chi

Publication

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Expression

Worm

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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ITPKA-mNeonGreen

Plasmid

#98883

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ITPKA

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJEP313-pAAV-CMV-SaCas9-DIO-pA

Plasmid

#113690

Purpose

A CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Publication

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 (NEWENTRY )

Use

AAV, CRISPR, and Cre/Lox

Tags

NLS

Promoter

Cytomegalo Virus(CMV)

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX330-TP53-1

Plasmid

#121917

Purpose

Encodes sgRNA targeting exon 4 of TP53

Depositor

William Hahn

Publication

Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TP53 (TP53 Human)

Use

CRISPR

Promoter

U6

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-SypHer2-DAAO-NES

Plasmid

#119184

Purpose

Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.

Depositor

Thomas Michel

Publication

Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

Insert

SypHer2-DAAO-NES

Use

AAV

Tags

NES

Expression

Mammalian

Promoter

CMV

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-132

Plasmid

#118518

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q79

Plasmid

#111729

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Publication

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q79 (polyQ repeat)

Promoter

CMV and P10

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTP261

Plasmid

#104166

Purpose

Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

Depositor

Dirk Görlich

Publication

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)

Tags

14x Histidine tag and NEDD8 from Brachypodium dis…

Expression

Bacterial

Mutation

1x Cysteine located at bps 493-495 in full sequen…

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-mVenus-Twin-Strep

Plasmid

#113891

Purpose

Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

Depositor

A. Radu Aricescu

Publication

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-mVenus-Twin-Strep

Promoter

CMV-MIE-TetO2

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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rLAMP1-mCherry-EGFP-Tet-On

Plasmid

#109015

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged mLAMP1 (lysosome marker)

Depositor

Jacob Corn

Publication

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

rLAMP1

Use

Lentiviral

Tags

mcherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSBY1_FnCpf1cg

Plasmid

#104622

Purpose

CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1

Depositor

Sheng Yang

Publication

Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

Insert

FnCpf1

Use

CRISPR

Expression

Bacterial

Mutation

codon-optimized C.glutamicum

Promoter

hsp60

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SMA-Cherry

Plasmid

#110209

Purpose

Mapping of mesenchymal progenitor cells.

Depositor

David Rowe

Publication

Grcevic et al Stem Cells. 2012 Feb;30(2):187-96. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry

Tags

None

Expression

Mammalian

Promoter

Smooth muscle alpha-actin and first intron

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-SPRTN-wt

Plasmid

#110214

Purpose

Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

Depositor

Ivana Marinovic-Terzic, Kristijan Ramadan

Publication

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Expression

Mammalian

Mutation

P296L (see depositor comments below)

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NA20 LN16

Plasmid

#101870

Purpose

Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

Depositor

Paul Clapham

Publication

Peters et al J Virol. 2004 Jul;78(13):6915-26.

Insert

Env (env )

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
SLC39A14-GFP

Plasmid

#104380

Purpose

Expresses SLC39A14/ZIP14 wild-type in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Publication

Liu et al J Biol Chem. 2017 Oct 6;292(40):16605-16

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SLC39A14 (SLC39A14 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCXN2-FLAG-AT2R-CFP

Plasmid

#101660

Purpose

Expresses AT2R with FLAG tag and CFP in mammalian cells.

Depositor

Yusuke Ohba

Publication

Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Angiotensin II type 2 receptor (AGTR2 Human)

Tags

ECFP and FLAG

Expression

Mammalian

Promoter

CAG promoter

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHyg-AID*-9myc

Plasmid

#99518

Purpose

C-terminal AID*-9myc degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Publication

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDicAID_nCas9-PmCDA-2A-NptII_ETR

Plasmid

#91695

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

Depositor

Akihiko Kondo

Publication

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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