We narrowed to 3,429 results for: Agt

1. pcDNA-HIRAmut-polyA

   Plasmid

   #59782

   Purpose

   This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   HIRA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Change from 'GATCGAAGTTTG' to 'GAc…

   Promoter

   CMV and T7

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-puro-mU6-CNPshRNA2

   Plasmid

   #126612

   Purpose

   Expresses shRNA against human CNP under mouse U6 promoter

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   CNP ShRNA-2
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Mouse U6 promoter

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLY103

   Plasmid

   #130937

   Purpose

   Engineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEA1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plux2

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSUPERIOR.puro-shREV3-6

   Plasmid

   #38031

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

   Insert

   shREV3-6 (REV3L human, Rat, Human)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pAWV

   Plasmid

   #62710

   Purpose

   Donor vector for amiR-eGFP knock-in via landing pad

   Depositor

   Masato Ohtsuka

   Article

   Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

   Insert

   tdTomato/amiR-eGFP123/amiR-eGFP419/FLPe
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-TO-g0 (FLP-IN)

   Plasmid

   #236120

   Purpose

   Plasmid encoding g0 guide RNA under control of CMV promoter with two TetR binding sites, used to equalize plasmid masses for transfection

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   g0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter with two TetR binding sites

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO-cfNHERF1_5

   Plasmid

   #195226

   Purpose

   shRNA to canine NHERF1.

   Depositor

   Keith Mostov, David Bryant

   Article

   Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

   Insert

   NHERF1 (SLC9A3R1 canine)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

   Plasmid

   #218640

   Purpose

   Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (Nlrp3 Mouse)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   delPYD (I125M-END)

   Promoter

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221 hNLRP3 R262W (disease-associated mutation)

   Plasmid

   #218647

   Purpose

   Gateway shuttling vector for cloning NLRP3-R262W (no stop codon)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (NLRP3 Human)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   R262W

   Promoter

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221 msNLRP3(ΔPYD, I125M-END)

    Plasmid

    #218641

    Purpose

    Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    delPYD (I125M-END)

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

    Plasmid

    #218636

    Purpose

    Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    H781E, Q782R, F785A

    Promoter

    UBC

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-GFP-2A-Puro-gETAA1_A

    Plasmid

    #211539

    Purpose

    sgRNA-A against ETAA1

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    sgRNA ETAA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-GFP-2A-Puro-gETAA1_B

    Plasmid

    #211540

    Purpose

    sgRNA-B against ETAA1

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    sgRNA ETAA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBT009.119.DA9

    Plasmid

    #206803

    Purpose

    Expresses DA9 scRNA for activation of pDA303

    Depositor

    James Carothers

    Article

    Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

    Insert

    DA9 scRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDT2

    Plasmid

    #199119

    Purpose

    circular transcription template

    Depositor

    Jeff Gelles

    Article

    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

    Insert

    lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …

    Promoter

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR-U6-gRNA-miniCMV-Nluc

    Plasmid

    #192657

    Purpose

    A plasmid encoding miniCMV sgRNA and miniCMV-promoter-Nluc

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Nluc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, miniCMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV:AsCpf1-2A-GFP-U6-MAFB-sg

    Plasmid

    #194718

    Purpose

    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hMAFB

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1 (MAFB Acidaminococcus sp.)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMpGE_En03-sgRNA-Target1 (MpRTN1)

    Plasmid

    #186291

    Purpose

    Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

    Insert

    sgRNA-Target1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330A-Prdm14-L4-#1

    Plasmid

    #171511

    Purpose

    deletion of a genomic locus in Prdm14 gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    Prdm14 (Prdm14 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMpGE010-sgRNA-Target1 (MpRTN1)

    Plasmid

    #186295

    Purpose

    Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

    Insert

    sgRNA-Target1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSP64T-ythdf1-3xflag

    Plasmid

    #164488

    Purpose

    Expresses zebrafish ythdf1 with 3x flag tag, for mRNA injection

    Depositor

    Antonio Giraldez

    Article

    Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

    Insert

    ythdf1 (ythdf1 Zebrafish)
    

    Use

    Production of mrna for zebrafish injection.

    Tags

    3x-flagtag

    Expression

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSP64T-ythdf3-3xflag

    Plasmid

    #164490

    Purpose

    Expresses zebrafish ythdf3 with 3x flag tag, for mRNA injection

    Depositor

    Antonio Giraldez

    Article

    Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

    Insert

    ythdf3 (ythdf3 Zebrafish)
    

    Use

    Production of mrna for zebrafish injection.

    Tags

    3x-flagtag

    Expression

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. RP51A-No5'ss-NoBS-NoPseudoBS

    Plasmid

    #100987

    Purpose

    RP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BS

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Ssh2 gRNA#3

    Plasmid

    #163399

    Purpose

    Cas9-mediated knockout of Ssh2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Ssh2 (Ssh2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Ssh3 gRNA#2

    Plasmid

    #163401

    Purpose

    Cas9-mediated knockout of Ssh3 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Ssh3 (Ssh3 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJAID6JY

    Plasmid

    #165049

    Purpose

    Integrative expression of TRX1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>TRX1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJAID8Z105

    Plasmid

    #165057

    Purpose

    Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJAID9L105

    Plasmid

    #165055

    Purpose

    Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJAID9L

    Plasmid

    #165052

    Purpose

    Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJAID5Z

    Plasmid

    #165047

    Purpose

    Integrative expression of AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge