We narrowed to 2,682 results for: KL

1. pCMV-Flag-YAP-5SA/S94A

   Plasmid

   #33103

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

   Insert

   YAP (YAP1 )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S61A, S94A, S109A, S127A, S164A, S381A

   Promoter

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMX-Gal4-TEAD4

   Plasmid

   #33105

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

   Insert

   TEAD4 (TEAD4 Human)
   

   Use

   Tags

   Gal4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX458-Ef1a-Cas9-H2B-GFP

   Plasmid

   #159654

   Purpose

   Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEJS476-pHAGE-TO-Nme dCas9 3XGFP-SgRNA/Telomere-All-in-one

   Plasmid

   #85716

   Purpose

   Expresses dNmeCas9-3XsfGFP and telomere-targeting Nme sgRNA

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Inserts

   Nme dCas9
   telomere sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO and U6

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDL025_HA-rb_IgG_HC_15F11

   Plasmid

   #193622

   Purpose

   HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti_HA_15F11 rabbit heavy chain
   

   Use

   Tags

   signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pDL026_HA-rb_IgG_LC_15F11

   Plasmid

   #193623

   Purpose

   HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-HA_15F11 rabbit light chain
   

   Use

   Tags

   signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pX458-Ef1a-Cas9-H2B-mCherry

   Plasmid

   #159655

   Purpose

   Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-h53BP1 (siRNA resistant)

   Plasmid

   #110301

   Purpose

   Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)

   Depositor

   Chris Kok-Lung Chan

   Article

   Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

   Insert

   p53-binding protein 1 (TP53BP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   GAACGAGGA to GAGCGGGGC

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 IKKE K38A flag

   Plasmid

   #26202

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   I-kappa-B kinase epsilon K38A (IKBKE Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Catalytic Lysine changed to Alanine (K38A)

   Promoter

   Available since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmAmetrine-DEVD-tdTomato

    Plasmid

    #18879

    Purpose

    Caspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomato

    Depositor

    Robert Campbell

    Article

    Ai et al Nat Methods. 2008 May . 5(5):401-3.

    Insert

    mAmetrine-DEVD-tdTomato (CASP3 lab constructed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAX-EGFP-FBP17

    Plasmid

    #178365

    Purpose

    Expresses FBP17 c-terminal labeled with EGFP in mammalian cells

    Depositor

    Erik Dent

    Article

    Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

    Insert

    Formin Binding Protein 17 (FNBP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta Actin

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. IRE1 alpha KA-pcDNA3.EGFP

    Plasmid

    #13010

    Depositor

    Fumihiko Urano

    Article

    Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

    Insert

    Inositol Requiring Enzyme-1 (ERN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R4L, K599A

    Promoter

    CMV

    Available since

    Nov. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-PE5max-SF3B1-K700E

    Plasmid

    #200317

    Purpose

    Expresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.

    Depositor

    Lili Wang

    Article

    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

    Inserts

    K700E-ng1+pg2
    PEmax-2A-MLH1dn
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-one

    Plasmid

    #85717

    Purpose

    Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Inserts

    Sp dCas9
    telomere sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO and U6

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mTagRFP-T-Golgi-7

    Plasmid

    #58018

    Purpose

    Localization: Golgi Complex, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. mTagRFP-T-LaminB1-10

    Plasmid

    #58020

    Purpose

    Localization: Nuclear Envelope, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pRDA_355

    Plasmid

    #187159

    Purpose

    Lentiviral expression of doxycycline inducible customizable Sp Cas9 sgRNA

    Depositor

    David Root, John Doench

    Article

    Aquilanti et al Neuro Oncol. 2023 Jul 6;25(7):1275-1285.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-PARP1-3xFlag-7xKQ

    Plasmid

    #111576

    Purpose

    Expresses full length PARP1 with K to Q mutations in automodification domain in mammalian cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    lysine to glutamine mutation at the following ami…

    Promoter

    CMV

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNAintron-CHIKV-Envelope protein

    Plasmid

    #215700

    Purpose

    Produces the Chikungunya virus envelope protein E3-E2-6K-E1

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV E3-E2-6K-E1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt

    Plasmid

    #175563

    Purpose

    pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

    Insert

    GOXL1
    

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    RUBY

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-SpCas9-GFP-U6-gRNA

    Plasmid

    #79144

    Purpose

    All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

    Depositor

    Jizhong Zou

    Article

    Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-SF3B1-K700E-reporter

    Plasmid

    #200318

    Purpose

    A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

    Depositor

    Lili Wang

    Article

    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

    Insert

    BSD-2A-EGFPi
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pH3mCHSH2

    Plasmid

    #101053

    Purpose

    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

    Depositor

    Jennifer Lodge

    Article

    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

    Insert

    Cryptococcus mCherry expression plasmid
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Cryptococcus Histone3 promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET30-ARH3-His-Sumo-HA

    Plasmid

    #111578

    Purpose

    Expresses ARH3 (amino acids 14-363) in E. coli

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    ARH3 (ADPRS Human)
    

    Use

    Tags

    6xHis-Sumo and HA

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-13

    Promoter

    T7

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PLKO-mcherry-luc-puro-Renilla cDNA

    Plasmid

    #29783

    Depositor

    Carl Novina

    Article

    Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

    Insert

    Renilla
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. BECLIN-HA WT

    Plasmid

    #46993

    Purpose

    Expresses Beclin1 in mammalian cells

    Depositor

    Kunliang Guan

    Article

    Russell et al Nat Cell Biol. 2013 Jul;15(7):741-50. do

    Insert

    becn1 (Becn1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

27. mOrange2-Mito-7

    Plasmid

    #57969

    Purpose

    Localization: Mitochondria, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

28. HA-sav

    Plasmid

    #32834

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    Sav (SAV1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pUAST td tomato Rab5 WT

    Plasmid

    #58446

    Purpose

    expression of drosophila td tomato Rab5

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab5 (Rab5 Fly)
    

    Use

    Tags

    td tomato

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

30. mTagRFP-T-Gamma-Tubulin-17

    Plasmid

    #58017

    Purpose

    Localization: Centrosomes, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Gamma-Tubulin (TUBG1 Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge