Addgene

We narrowed to 15,192 results for: genome

Showing: 1721 - 1760 of 15192 results

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pDB4279

Plasmid

#98698

Purpose

A control plasmid containing the intact bsdMX marker

Depositor

Li-Lin Du

Article

Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

Insert

Cas9

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pJJH1260

Plasmid

#36913

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Inserts

ScURA3
ScURA3

Use

Tags

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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pJFRC157-20XUAS-IVS-B3::PEST

Plasmid

#32136

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B3::PEST

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pJFRC160-21XUAS-B3RT>-dSTOP-B3RT>-myr::RFP

Plasmid

#32139

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B3RT> STOP STOP B3RT>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pZD-CJ1111

Plasmid

#175058

Purpose

rbcLXS6803 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL6803

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Tags

Expression

Mutation

Promoter

rbcLXS

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP

Plasmid

#32145

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

loxP> STOP STOP loxP>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pCMVJ-scTALEN-ABL1-R

Plasmid

#61695

Purpose

Expresses a single-chain TALEN targeting ABL1 site in human cells

Depositor

Huimin Zhao

Article

Sun et al Mol Biosyst. 2014 Mar 4;10(3):446-53. do

Insert

scTALEN-ABL1-R

Use

Tags

Flag and SV40 NLS

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2015

Availability

Academic Institutions and Nonprofits only
pBN449

Plasmid

#129555

Purpose

GAL4-induced expression of codon optimised FLP recombinase in C. elegans

Depositor

Peter Askjaer

Article

Ayuso et al MicroPubl Biol. 2019 Jan 29;2019:10.1791

Insert

15xUAS

Use

Tags

FLP and mNeonGreen

Expression

Worm

Mutation

Promoter

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCJ111

Plasmid

#175052

Purpose

rbcLXS7002 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Tags

Expression

Mutation

Promoter

rbcLXS

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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RCASBP-Y NHA-EGFP

Plasmid

#11481

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Enhanced Green Fluoresecent Protein

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 22, 2006

Availability

Academic Institutions and Nonprofits only
pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB

Plasmid

#30505

Depositor

Ichiro Matsumura

Article

Murin et al Appl Environ Microbiol. 2011 Oct 21.

Insert

beta-glucuronidase (gusA Escherichia coli)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pTAL7b

Plasmid

#48706

Purpose

TALEN expression in mammalian cells

Depositor

Boris Greber

Article

Frank et al BMC Genomics. 2013 Nov 9;14(1):773.

Type

Empty backbone

Use

TALEN

Tags

FokI nuclease

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pRVV655

Plasmid

#119019

Purpose

Drives TP901-1 recombinase expression in the D. melanogaster germ line

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 recombinase

Use

Tags

SV40 NLS

Expression

Insect

Mutation

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pTol2-DRv7

Plasmid

#78627

Purpose

GESTALT V7 barcode for zebrafish

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V7

Use

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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ttHsCas12a

Plasmid

#182385

Purpose

Gives high editing efficiency in barley.

Depositor

Wendy Harwood

Article

Lawrenson et al bioRxiv 2022.04.28.489853

Insert

ttHsCas12a

Use

CRISPR; Coding sequence only

Tags

Expression

Mutation

D156R

Promoter

None

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFH13

Plasmid

#125798

Purpose

Level0 SpCas9, wheat codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

SpCas9, wheat codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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UAST-LbCas12a

Plasmid

#140621

Purpose

Gal4 dependent expression of LbCas12a in Drosophila

Depositor

Michael Boutros

Article

Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

Insert

LbCas12a

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

UAST

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJFRC153-20XUAS-IVS-B2::PEST

Plasmid

#32134

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B2::PEST

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1259

Plasmid

#61251

Purpose

Cas9 plasmid with sgRNA deleted; for use in C. elegans

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

Deletion

Use

CRISPR

Tags

Expression

Worm

Mutation

Deleted sgRNA(F+E) sequence from pJW1219

Promoter

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFH17

Plasmid

#125803

Purpose

Level0 LbCas12a, rice codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

LbCas12a, rice codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAL7a

Plasmid

#48705

Purpose

TALEN expression in mammalian cells

Depositor

Boris Greber

Article

Frank et al BMC Genomics. 2013 Nov 9;14(1):773.

Type

Empty backbone

Use

TALEN

Tags

FokI nuclease

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTALEN_v2 (NG)

Plasmid

#32190

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Nuclease, NG for 0.5 repeat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pSK23

Plasmid

#72851

Purpose

template for C-terminal eGFP tagging, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

copia

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCSD_N-SpCas9-1-573-FRB-1xNLS

Plasmid

#107298

Purpose

Expresses N terminal SpCas9 (1-573 aa) fused to FRB in mammalian cells

Depositor

Scot Wolfe

Insert

Truncated SpCas9 (aa 1-573) fused to FRB

Use

CRISPR

Tags

FRB and SV40 NLS

Expression

Mammalian

Mutation

Truncated SpCas9 (aa 1-573)

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pZHY013

Plasmid

#36185

Depositor

Daniel Voytas

Article

Zhang et al Plant Physiol. 2012 Nov 2.

Insert

Heterodimeric FokI nuclease

Use

Entry vector for expression in plants as a t2a li…

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.3TOPO-T7-hCas9

Plasmid

#161876

Purpose

In vitro transcription of SpCas9

Depositor

Stephane Pelletier

Article

Pelletier et al Immunity. 2015 Jan 20;42(1):18-27. doi:

Insert

human codon optimized SpCas9

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP974-1

Plasmid

#99497

Purpose

TEV::linker::tagRFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagRFP::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTALEN_v2 (HD)

Plasmid

#32192

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Nuclease, HD for 0.5 repeat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pTF-FLPe

Plasmid

#139672

Purpose

Expression cassette for FLPe recombinase in T-DNA vector

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

FLPe recombinase

Use

Tags

Expression

Plant

Mutation

Promoter

Maize Ubiquitin

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-AAVS1-sg

Plasmid

#194716

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hAAVS1 locus

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1 (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPhygCAS9

Plasmid

#63555

Purpose

Expresses CAS9 in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

Insert

Humanized Streptococcus pyogenes Cas9 from PX330 (Addgene)

Use

Leishmania expression

Tags

flag

Expression

Mutation

Promoter

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAND

Plasmid

#49377

Purpose

Expression of AND gate in bacteria

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

AND gate

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH31

Plasmid

#125884

Purpose

Level0 Wheat TaU3 Pol III promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Wheat TaU3 Pol III promoter

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
U6>sgRNA(F+E)

Plasmid

#59986

Purpose

Empty vector for sgRNA cloning (F+E modified backbone)

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ciinte.U6

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGRNA2e

Plasmid

#169782

Purpose

Insert one gRNA

Depositor

Sang-Gyu Kim

Article

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGRNA1

Plasmid

#169778

Purpose

Insert one gRNA

Depositor

Sang-Gyu Kim

Article

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFF145

Plasmid

#61446

Purpose

aTc-inducible Beta recombinase

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

Beta recombinase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pTetO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSK41

Plasmid

#74886

Purpose

template for insertion of attP site (C-terminal tag plasmid design), Puromycin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

attP site

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH22

Plasmid

#125808

Purpose

Level0 xCas9 3.7

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

xCas9 3.7

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RCASBP-Y CHA

Plasmid

#11480

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Gateway ccdB reading frame cassette

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 22, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge

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