We narrowed to 4,840 results for: phy

1. pFlagCMV2-UbcH13

   Plasmid

   #12460

   Depositor

   Dong-Er Zhang

   Article

   Zou et al Biochem Biophys Res Commun. 2005 Oct 14.

   Insert

   Ubc13 (UBE2N Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.0-HA-UbcH13

   Plasmid

   #12461

   Depositor

   Dong-Er Zhang

   Article

   Zou et al Biochem Biophys Res Commun. 2005 Oct 14.

   Insert

   Ubc13 (UBE2N Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pH_35S/mEGFP

   Plasmid

   #135321

   Purpose

   mEGFP expression regulated by the CaMV35S promoter, co-expressed hygromycin phosphotransferase selectable marker (hpt)

   Depositor

   Mathias Pribil

   Article

   Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGBMCS-SortA

   Plasmid

   #21931

   Depositor

   Fuyuhiko Inagaki

   Article

   Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

   Insert

   sortase (srtA Staphylococcus aureus (strain N315))
   

   Use

   Tags

   GB1 and His-tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pCfB8748

   Plasmid

   #126918

   Purpose

   Plasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtE

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtE
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMOS019: ClopHensorN chloride sensor (cytosolic)

   Plasmid

   #163048

   Purpose

   ClopHensorN chloride sensor (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   ClopHensorN
   

   Use

   Lentiviral

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hSDH5

   Plasmid

   #113046

   Purpose

   Expression of WT hSDH5

   Depositor

   Edward A. Berry

   Article

   Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

   Insert

   hSDH5 (SDHAF2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Insert begins at amino acid 39

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. VinculinTS-A50I

   Plasmid

   #111827

   Purpose

   Vinculin tension sensor with point mutation affecting ability to bind talin

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (A50I)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed alanine 50 to isoleucine

   Promoter

   CMV

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV2-GCaMP6f-P2A-PAmCherry

   Plasmid

   #133412

   Purpose

   Fluorescent reporter for calcium imaging and photoactivation.

   Depositor

   Tim Holy

   Article

   Lee et al Science. 2019 Dec 13;366(6471):1384-1389

   Insert

   GCaMP6f-P2A-PAmCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. VV073: 1xCox8 - GCaMP5G in fubi

    Plasmid

    #58509

    Purpose

    Expresses the calcium sensor GCaMP5G in the mitochondria

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    1xCox8 - GCaMP5G
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. VinculinTS-I997A

    Plasmid

    #111828

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind actin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed isoleucine 997 to alanine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTR47m4

    Plasmid

    #104068

    Purpose

    Luciferase Reporter for Formaldehyde Sensing

    Depositor

    David Liu

    Article

    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

    Inserts

    xluxAB
    frmR
    frm Promoter m4
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    23r and 25th nucleotides in sequence mutated from…

    Promoter

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMOS008E: Entry vector for: GCaMP6F calcium sensor (cytosolic)

    Plasmid

    #163064

    Purpose

    Entry vector for: GCaMP6F calcium sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMP6F
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETNb-nALFA

    Plasmid

    #159988

    Purpose

    For the protein expression with ALFA tag at N-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-PRSX8 eArchT3.0-EYFP

    Plasmid

    #89538

    Purpose

    Expresses eArchT 3.0-eYFP in Phox2 (including catecholamine) neurons

    Depositor

    Ruth Stornetta

    Article

    Burke et al J Physiol. 2015 Jul 1;593(13):2909-26. d

    Insert

    eArchT 3.0
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    PRSx8

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCfB8786

    Plasmid

    #126920

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMOS018: ratiometric pHluorin (mitchondrial)

    Plasmid

    #163053

    Purpose

    ratiometric pHluorin (mitchondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    ratiometric pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTH1

    Plasmid

    #84451

    Purpose

    shuttle plasmid for sarA P1-dsRED expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    DsRed.T3(DNT)
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mEmerald-PCNA-19

    Plasmid

    #54221

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

    Insert

    PCNA-19 (PCNA Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

20. hSDH5_G78R

    Plasmid

    #113047

    Purpose

    Expression of mutant hSDH5

    Depositor

    Edward A. Berry

    Article

    Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

    Insert

    hSDH5_G78R (SDHAF2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G78R, Insert begins at amino acid 39

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. hSDH5_C83D

    Plasmid

    #113048

    Purpose

    Expression of mutant hSDH5

    Depositor

    Edward A. Berry

    Article

    Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

    Insert

    hSDH5_C83D (SDHAF2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C83D, Insert begins at amino acid 39

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRRL-Vinculin-Venus

    Plasmid

    #111833

    Purpose

    Vinculin (chicken) tagged with VenusA206K at the C-terminus in lentiviral expression vector

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    Vinculin-Venus (VCL Chicken)
    

    Use

    Lentiviral

    Tags

    VenusA206K

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBAD-jRCaMP1a

    Plasmid

    #113936

    Purpose

    Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

    Depositor

    Robert Campbell, Thomas Hughes

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    jRCaMP1a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TfRΔEctoR1-eGFP A206K

    Plasmid

    #113554

    Purpose

    Transmembrane model receptor to probe receptor internalization via clathrin mediated pathway

    Depositor

    Jeanne Stachowiak

    Article

    DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

    Insert

    TfRΔEctoR1-eGFP A206K
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    eGFP A206K

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMOS018E: Entry vector for: ratiometric pHluorin (mitchondrial)

    Plasmid

    #163070

    Purpose

    Entry vector for: ratiometric pHluorin (mitchondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    ratiometric pHluorin
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 53BP1 N-terminal sgRNA

    Plasmid

    #207091

    Purpose

    pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GGGGAGCAGATGGACCCTAC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV2-GCaMP5g-P2A-PAmCherry

    Plasmid

    #133411

    Purpose

    Fluorescent reporter for calcium imaging and photoactivation.

    Depositor

    Tim Holy

    Article

    Lee et al Science. 2019 Dec 13;366(6471):1384-1389

    Insert

    GCaMP5g-P2A-PAmCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEF/myc/ER FLII12Pglu-700uDelta6

    Plasmid

    #17867

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    FLII12Pglu-700uDelta6
    

    Use

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    700uDelta6, ECFP insert in mglB

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYPQ154

    Plasmid

    #69305

    Purpose

    Gateway entry vector with AteCas9 (Arabidopsis codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    AteCas9 (Arabidopsis codon optimized)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    NLS fusion

    Promoter

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHMattR3-attR4-GFP

    Plasmid

    #196247

    Purpose

    pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPAiRFP1-N1

    Plasmid

    #44272

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

    Insert

    PAiRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G127D/S141R/M163L/Q168L/A203V/G218S/R220P/V244F/A…

    Promoter

    CMV

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mEos2-H2B-6

    Plasmid

    #57384

    Purpose

    Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pFBNb-cALFA

    Plasmid

    #159990

    Purpose

    For the protein expression with ALFA tag at C-terminus in insect cells

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMOS028E: Entry vector for: Mitycam calcium sensor (mitochondrial)

    Plasmid

    #163065

    Purpose

    Entry vector for: Mitycam calcium sensor (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Mitycam
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28-Rsn2

    Plasmid

    #61686

    Purpose

    bacterial expression of ranaspumin 2 as a His-tagged fusion protein

    Depositor

    Brian Smith

    Article

    Mackenzie et al Biophys J. 2009 Jun 17;96(12):4984-92. d

    Insert

    Ranaspumin-2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBAD-R-GECO1.2

    Plasmid

    #113937

    Purpose

    Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

    Depositor

    Robert Campbell, Thomas Hughes

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    R-GECO1.2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMYO127TAG-SepT

    Plasmid

    #34625

    Depositor

    Dieter Söll, Jesse Rinehart

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    Myoglobin-127TAG
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    codon-optimized + an amber stop codon was introdu…

    Promoter

    Ipp

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pISH-Th

    Plasmid

    #105988

    Purpose

    To prepare riboprobe for in situ hybridization

    Depositor

    Peter Mombaerts

    Article

    Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

    Insert

    tyrosine hydroxylase (Th Mouse)
    

    Use

    To generate riboprobe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MAC_C_RYK

    Plasmid

    #187790

    Purpose

    MAC-tagged gene expression of human RYK

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    RYK (RYK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pS24·Pm-GFP

    Plasmid

    #122594

    Purpose

    Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    Pm promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge