We narrowed to 15,323 results for: pET
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Plasmid#158791PurposeBacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.DepositorInserteIF4G1 (codon optimized)
Tags6xHIS and SUMOExpressionBacterialMutationamino acids 1-200PromoterT7Available SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-baPrs-hdNadV
Plasmid#91950PurposepET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyiDepositorInsertsPutative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases
ExpressionBacterialMutationchanged Leucine 135 to IsoleucinePromoterT7Available SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-HpENR
Plasmid#12651DepositorInsertnoyl-acyl carrier protein reductase (fabI Helicobacter pylori)
ExpressionBacterialAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization Control
Plasmid#186553PurposeNegative Control mediating endosomal localizationDepositorInsert10R-8His-EGFP
Tags8xHisExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-3xTS
Plasmid#162777PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialPromoterT7Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b-mLin28A-cHIS
Plasmid#137998PurposeLin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cellsDepositorInsertmouse Lin28A, CSD and ZnFs (residues 36–186)
TagsC-terminal His6 tagExpressionBacterialAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29b-EPN01*-His
Plasmid#84509PurposeE.coli version of EPN01*DepositorInsertEPN01*-His_pET29b
TagsHis tagExpressionBacterialAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
5787 pET28aSX104B/ pES42
Plasmid#1229DepositorAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET14b PHAS-I F113A
Plasmid#15680DepositorAvailable SinceSept. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-PTEN
Plasmid#168032PurposeExpresses eCFP-PTEN(M265-N329)-mVenus in bacterial cellsDepositorInserteCFP-PTEN(M265-N329)-mVenus
TagsHisx6 and mycExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-pro-sumo3
Plasmid#25103DepositorAvailable SinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET11a-dH3-dH4
Plasmid#105502PurposeCo-expression of Drosophila histones H3 and H4DepositorInsertdH3-dH4
Available SinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-ClbPpep-CHis
Plasmid#48243PurposeFirst 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domainDepositorInsertClbP-pep
Tags6-HisExpressionBacterialMutationFirst 375 amino acids of the peptidase, which con…PromoterT7Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFS_0143-pET30-Fusicatenibacter_saccharivorans
Plasmid#116952PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available SinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Y986H
Plasmid#174801PurposeBacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overallDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Y986S
Plasmid#174802PurposeBacterial expression of a PARP1 mutant that produces mainly short PAR oligomersDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-G972R
Plasmid#174800PurposeBacterial expression of a PARP1 mutant that produces less PAR oligomers overallDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx C35S
Plasmid#36947DepositorInsertThioredoxin-1 C35S mutation (TXN Human)
TagsHis tagExpressionBacterialMutationcys 35 to serinePromoterT7Available SinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_14
Plasmid#194675PurposeProtein production of the split HaloTag based calcium recorder Caprola_14 in bacteriaDepositorInsertCaprola_14
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_15
Plasmid#194676PurposeProtein production of the split HaloTag based calcium recorder Caprola_15 in bacteriaDepositorInsertCaprola_15
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30-hDlg PDZ2
Plasmid#21692DepositorInserthDlg/SAP97 PDZ2 domain (DLG1 Human)
TagsHis6ExpressionBacterialMutationhuman hDlg/SAP97 PDZ2 domain (residues 318-405)Available SinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6_rpoA-C
Plasmid#190966PurposeTest the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
TagsHisExpressionBacterialPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-FB-E25TAG-F6C
Plasmid#176546PurposeExpresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 positionDepositorInsertIgG affinity protein
TagsHis tagExpressionBacterialMutationchange phenylalanine 6 to cysteine; change glutam…PromoterT5Available SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmBelle_B
Plasmid#145963PurposeBacterial Expression of DmBelleDepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-90) (72P)
Plasmid#62080PurposeExpression of human p53 (1-90) (72P) in e. coliDepositorInsertp53 (TP53 Human)
TagsHisExpressionBacterialMutationcontains TAD/PP residues 1-90; 72PPromoterT7Available SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET51b-NAD-cpLuc
Plasmid#135628PurposeBacterial expression of a biosensor for measuring NAD+ levels at the point of care (V1)DepositorInsertNAD-cpLuc sensor
TagsHisx10 and StrepExpressionBacterialPromoterT7Available SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del4 pET28C
Plasmid#122118PurposeExpression Brg1 amino acids 1446-1647 in bacteriaDepositorAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-ChE1 v4.5
Plasmid#185755PurposeBacterial expression of ChE1 v4.5DepositorInsertChE1 v4.5
TagsHis10ExpressionBacterialPromoterT7Available SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETa(+) HpCsd3 (42-403)
Plasmid#71285PurposeExpresses Csd3 from Helicobacter pyloriDepositorAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3992 pET ubcH5
Plasmid#8652DepositorInsertUbcH5 (UBE2D1 Human)
ExpressionBacterialAvailable SinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET-His-Tre
Plasmid#123129PurposeBacterial expression plasmid for Tre with an N-terminal His-tag.DepositorInsertTre recombinase
Tags6x His tagExpressionBacterialPromoterT7Available SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
HOXC13(human)-pET
Plasmid#8679DepositorInsertHOXC13 (HOXC13 Human)
TagsHis and T7ExpressionBacterialMutationmakes protein by Coomassie blue stainingAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pETpRha-alpha-SkTm
Plasmid#72929PurposeAllows expression of rabbit alpha-skeletal tropomyosin from a rhamnose inducible promoterDepositorInsertalpha skeletal Tropomyosin
ExpressionBacterialPromoterrhaBAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
DJ-1 TEV site pET15b
Plasmid#60687PurposeRecombinant protein expression of Park7DepositorInsertDJ-1 (PARK7 Human)
Tags6xHisExpressionBacterialMutationTEV site was inserted (see comments below).PromoterT7Available SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-Prp18
Plasmid#111354Purposeprotein purificationDepositorInsertPRP18
ExpressionBacterialAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-SUMO-KaiA
Plasmid#68049PurposeStrain: AM5115, Used for protein purificationDepositorInsertKaiA
Tags6xHis and SUMOExpressionBacterialPromoterT7Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del1 pET28a
Plasmid#122115PurposeExpression Brg1 amino acids 1-381 in bacteriaDepositorAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-K292A
Plasmid#134575PurposeExpress human DDX43 mutant (K292A, ATPase dead) in E. coliDepositorInsertDDX43-K292A (DDX43 Human)
TagsHisExpressionBacterialMutationfull-length (K292A)PromoterT7Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-SUMO-KaiB
Plasmid#68050PurposeStrain: AM5116, Used for protein purificationDepositorInsertKaiB
Tags6xHis and SUMOExpressionBacterialPromoterT7Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-2HMG-eGFP
Plasmid#112737PurposeExpress 2HMG-eGFP in E. coliDepositorInsert2HMG-eGFP
Tags6xHisExpressionBacterialPromoterT7 PromoterAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-24a-STE2
Plasmid#133430PurposeYeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.DepositorInsertSTE2 (STE2 Budding Yeast)
ExpressionBacterialMutationBoth native Cys (C59S/C252A) have been mutated. S…Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-G415R
Plasmid#122693PurposeExpress His-tagged PKM2 (G415R) protein in bacteria.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
CNNM3-CBS-YPet
Plasmid#149686PurposeFor the FRET-based binding assay between human CNNM3 CBS domain and PRL2.DepositorInsertCNNM3-CBS-YPet (CNNM3 Human, Synthetic)
Tags8 histidine tagExpressionBacterialPromoterT7Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet 3d alpha 1 beta 2 CP
Plasmid#13450DepositorAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-2HMG-mCherry
Plasmid#112739PurposeExpress 2HMG-mCherry in E. coliDepositorInsert2HMG-mCherry
Tags6xHisExpressionBacterialPromoterT7 PromoterAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b-NAD-Luc
Plasmid#135627PurposeBacterial expression of a biosensor for measuring NAD+ levels at the point of care (V0)DepositorInsertNAD-Luc sensor
TagsHisx10 and StrepExpressionBacterialPromoterT7Available SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Beclin1 (1-242)
Plasmid#24401DepositorInsertBeclin1 (BECN1 Human)
TagsHisExpressionBacterialMutationContains amino acids 1-242 only.Available SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 P186C
Plasmid#66173PurposeExpresses the P186C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
TagsHis6 tagExpressionBacterialMutationP186CPromoterT7 promoterAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a- hPTP1e-PDZ2
Plasmid#20377DepositorInserthPTP1e PDZ2 (PTPN13 Human)
ExpressionBacterialAvailable SinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP3-His
Plasmid#67866Purposebacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tagDepositorInsertMRPP3 (KIAA0391 Human)
TagsHisExpressionBacterialMutationAmino acid 46 of recombinant MRPP3 is preceded by…PromoterT7Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only