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Plasmid#53364PurposeWill convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.DepositorInsertcbfd1
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pITC
Plasmid#192856PurposeCloning and expression of transcription factor gene under control of naringenin inducible promoterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPfdeARAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHC79
Plasmid#167551PurposeBackbone plasmid to pPM1001, pPM1002, pPM1003, pPM2212 and pPM2213DepositorTypeEmpty backboneUseCosmidTagsExpressionMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
K-SPOTIT1.0
Plasmid#171215PurposeExpresses a kappa-opioid receptor-based circular permuted green fluorescent protein sensorDepositorInsertK-SPOTIT1.0
UseLentiviralTagsFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHA1
Plasmid#85709PurposeThis plasmid provides a BAC vector backbone, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorTypeEmpty backboneUseCre/Lox; Herpesvirus cloningTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBlueKan+cysMPro
Plasmid#187879PurposeEscherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterCysMAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCoofy64
Plasmid#122016PurposeBaculovirus vector for parallel SLIC cloning containing gp67 signal sequence, N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionInsectMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep
Plasmid#173899PurposeThe pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThrDepositorInsertsThrRS(v1.0)
tRNA(v2.0)CUA
pduX
EF-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG029 (mzaABCDE)
Plasmid#157907PurposeExpresses the mzaABCDE defense systemDepositorInsertmzaABCDE (5-gene system containing a MutL homolog)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG015 (RADAR)
Plasmid#157893PurposeExpresses the RADAR defense systemDepositorInsertRADAR subtype containing auxillary gene rdrD
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDL1
Plasmid#182263PurposeGibson cloning vector for synthesis of single and double stranded RNADepositorTypeEmpty backboneUseGibson cloning vector for synthesis of single and…TagsExpressionMutationPromoterT3 and SP6 for sense and antisense riboprobe synt…Available sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAC-DsRed-QF2-hsp70
Plasmid#104876PurposeGeneral purpose vector for cloning enhancer/promoter regions to drive QF2 expression; used for mosquitoDepositorInsertQF2
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCoofy63
Plasmid#122015PurposeBaculovirus vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionInsectMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-bc339
Plasmid#190848PurposeFor mammalian expression of bc339, the RNA aptamer of β-catenin. Expression is driven by a U6 promoter.DepositorInsertbc339 (an RNA aptamer of β-catenin)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBUN279
Plasmid#60674Purposeintegration of M. smegmatis ald gene in Mycobacterium using mycobacteriophage L5 attB siteDepositorInsertald
UseE.coli-mycobacterium integration-proficient vectorTagsExpressionMutationPromoterAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT440
Plasmid#8609DepositorInsertbarnase, barstar
UseTagsas pMT416ExpressionBacterialMutationV36 codon of barnase of pMT416 replaced by polyli…PromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMT-GFP-Pat coiled-coil (cc)
Plasmid#31757DepositorInsertPatronin (Patronin Fly)
UseTagsGFPExpressionInsectMutationContains only the coiled-coil domain: aa 535-1457PromoterMetallothioneinAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSU19mCherry
Plasmid#225181PurposeMedium copy cloning vector with mCherry integrated in the multiple cloning site. There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT031
Plasmid#225177PurposeLow copy cloning vector with mCherry integrated in the multiple cloning site (AmpR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT034
Plasmid#225180PurposeLow copy cloning vector with mCherry integrated in the multiple cloning site (SmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only