We narrowed to 1,267 results for: piggyBac

1. pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro

   Plasmid

   #129537

   Purpose

   PiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

   Depositor

   Hideyuki Okano

   Article

   Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

   Insert

   tetR-3xFlag-HA-2A-Puro
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   hPGK promoter

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-tight-DDdCas9GFP-IRES-Neo

   Plasmid

   #102907

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LZ10 PBREBAC-H2BHalo

   Plasmid

   #91564

   Purpose

   piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.

   Depositor

   James Zhe Liu

   Article

   Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1

   Insert

   histone H2B-HaloTag
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. BII-Sh-FUCCI-Luc

   Plasmid

   #133364

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Clover-Geminin-IRES-mKO2-Cdt1
   

   Use

   Tags

   HA-tagged Clover-Geminin

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

   Plasmid

   #205565

   Purpose

   Express human NEDD4L (delta C2 isoform)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   NEDD4L (NEDD4L Human)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   delta C2 isoform

   Promoter

   EF1

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAEL010097-Cas9

   Plasmid

   #100707

   Purpose

   The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

   Inserts

   AAEL010097-hspcas9-GFP
   Opie2-dsRed
   

   Use

   CRISPR

   Tags

   GFP and dsRED

   Expression

   Insect

   Mutation

   Promoter

   AAEL010097 and Opie2

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSH231-EF1-GFP-HYGRO

   Plasmid

   #115144

   Purpose

   Safe harbor site 231 knock-in vector with GFP-HygromycinR expression cassette

   Depositor

   Michael Phelps, Raymond Monnat

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSH231-EF1-RFP-HYGRO

   Plasmid

   #115145

   Purpose

   Safe harbor site 231 knock-in vector with RFP-HygromycinR expression cassette

   Depositor

   Michael Phelps, Raymond Monnat

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   RFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC150-FAM134C-GFP

   Plasmid

   #201932

   Purpose

   PiggyBac Vector expressing FAM134C-GFP

   Depositor

   Wade Harper

   Article

   Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

   Insert

   FAM134C (RETREG3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-TRE-dCas9-Zim3-MeCP2

    Plasmid

    #220143

    Purpose

    doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

    Depositor

    Frank Buchholz

    Article

    Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

    Insert

    dCas9-Zim3-MeCP2
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tre-3G

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB[vasa-Cas9 PUb-ZsGreen]

    Plasmid

    #169012

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster vasa

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKH151_PB_flag-LC3B_wt_IRES-BFP

    Plasmid

    #214976

    Purpose

    Flag tagged LC3B

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    FLAG-LC3B (MAP1LC3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKH152_PB_flag-LC3B_LDSmut_IRES-BFP

    Plasmid

    #214977

    Purpose

    Flag tagged LDS mutant LC3B

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    FLAG-LC3B_mut (MAP1LC3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    LDS mutant

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-Hyg-Dest-mCherry-PAR-6B

    Plasmid

    #194908

    Purpose

    To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

    Depositor

    Clare Buckley

    Article

    Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

    Insert

    PAR-6B (Pard6b Mouse)
    

    Use

    Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    39 C to G, silent mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAEL005635-Cas9

    Plasmid

    #100608

    Purpose

    The promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL005635-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL005635 and Opie2

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. EK0379 CMV-TO-EGFP-stop-t70587 (PB)

    Plasmid

    #191154

    Purpose

    Expression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    EGFP-t70587
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-REVERBA-mScarlet-NLS-PEST

    Plasmid

    #190658

    Purpose

    Fluorescent reporter for circadian rhythms

    Depositor

    Mary Teruel

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

    Insert

    Reverba-mScarlet (Nr1d1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-EF1-IRES-NEO deleted ITR

    Plasmid

    #67906

    Purpose

    PiggyBac transposon encoding Neomycin resistance with deleted inverted terminal repeat

    Depositor

    Alex Kentsis

    Article

    Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

    Insert

    NeoR (neoR Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC150-KEIMA-REEP5

    Plasmid

    #201928

    Purpose

    PiggyBac Vector expressing KEIMA-REEP5

    Depositor

    Wade Harper

    Article

    Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

    Insert

    REEP5 (REEP5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC150-TEX264-GFP

    Plasmid

    #201931

    Purpose

    PiggyBac Vector expressing TEX264-GFP

    Depositor

    Wade Harper

    Article

    Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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