Addgene

We narrowed to 15,192 results for: genome

Showing: 1761 - 1800 of 15192 results

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pRB39

Plasmid

#72870

Purpose

template for N-terminal eGFP tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pRB40

Plasmid

#72865

Purpose

template for N-terminal eGFP tag, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH56

Plasmid

#125882

Purpose

Level0 Rice OsACT1 Pol II promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Rice OsAct1 Pol II promoter

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPR

Plasmid

#113252

Purpose

pUC19 backbone with E. coli CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

E. coli CRISPR array

Use

Integration substrate

Tags

Expression

Mutation

Promoter

NA

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pTALEN_v2 (NI)

Plasmid

#32189

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Nuclease, NI for 0.5 repeat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pKTol2P-PTK

Plasmid

#85599

Purpose

Tol2 transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Tol2 transposon with a puromycin-thymidine kinase fusion

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Sept. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pFF714

Plasmid

#61449

Purpose

IPTG-inducible SCRIBE_galK(OFF)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(OFF)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pWT055g

Plasmid

#96864

Purpose

sgRNA-HEK3

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-HEK3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFH57

Plasmid

#125883

Purpose

Level0 Soybean GmUBI Pol II promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Soybean GmUbi Pol II promoter

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC170-3XUAS-IVS-Cre::PEST

Plasmid

#32144

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

Cre::PEST

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pTALETF_v2 (NN)

Plasmid

#32185

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Transcriptional Activator Backbone, NN for 0.5 repeat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIG-RhoC

Plasmid

#14119

Depositor

Richard Hynes

Article

Clark et al Nature. 2000 Aug 3. 406(6795):532-5.

Insert

Human RhoC (RHOC Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
4RFQ

Plasmid

#64656

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

METTL18:PBC004-E03:C231318 (METTL18 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

May 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits
RCASBP-Y NHA

Plasmid

#11479

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Gateway ccdB reading frame cassette

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 22, 2006

Availability

Academic Institutions and Nonprofits only

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pKF292

Plasmid

#72856

Purpose

template base vector for N-terminal tagging, inducible Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for Nterm-tag templates

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW091

Plasmid

#113253

Purpose

pUC19 backbone with S. pyogenes CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes CRISPR array

Use

Integration substrate

Tags

Expression

Mutation

Promoter

NA

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTALEN_v2 (NN)

Plasmid

#32191

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Nuclease, NN for 0.5 repeat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP997-1

Plasmid

#99500

Purpose

3XFlag::tagRFP::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagRFP::linker::TEV

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC168-21XUAS-RSRT>-dSTOP-RSRT>-myr::RFP

Plasmid

#32143

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

RSRT> STOP STOP RSRT>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB34

Plasmid

#72862

Purpose

template for N-terminal 3x FLAG tag, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

3xFlag tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC173-20XUAS-IVS-Dre::PEST

Plasmid

#32146

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

Dre::PEST

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol2-DRv6

Plasmid

#78628

Purpose

GESTALT V6 barcode for zebrafish

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V6

Use

Tags

Expression

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC158-3XUAS-IVS-B3::PEST

Plasmid

#32138

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B3::PEST

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
CAGGS-AsCpf1(D908A)-2A-GFP-U6-AAVS1-sgRNA

Plasmid

#194727

Purpose

CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector, with guide RNA array targeting hAAVS1.

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1(D908A) (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGMV-gSWEET4

Plasmid

#112796

Purpose

Universal BeYDV-derived vector with gRNAs targeting the VviSWEET4 gene

Depositor

Humberto Prieto

Article

Prieto et al In: Cantu D., Walker M. (eds) The Grape

Insert

gRNA pair targeting VviSWEET4

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTALETF_v2 (HD)

Plasmid

#32188

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Transcriptional Activator Backbone, HD for 0.5 repeat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT055h

Plasmid

#96867

Purpose

sgRNA-HEK4

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-HEK4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTALETF_v2 (NG)

Plasmid

#32186

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Transcriptional Activator Backbone, NG for 0.5 repeat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC176-10XUAS-rox>-dSTOP-rox>-myr::GFP

Plasmid

#32147

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

rox> STOP STOP rox>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP975-1

Plasmid

#99498

Purpose

TEV::linker::tagBFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagBFP::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKF297

Plasmid

#74774

Purpose

template for C-terminal TEV-site + 2x V5-tag (short version), Puromycin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

TEV- 2xV5 tag (short version)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pZYIntExc

Plasmid

#139673

Purpose

Expression cassette for phiC31 Excisionase in T-DNA vector

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

phiC31 Excisionase

Use

Tags

Expression

Plant

Mutation

Promoter

Maize Ubiquitin

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT055e

Plasmid

#96866

Purpose

sgRNA-EMX1

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-EMX1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP546-2

Plasmid

#99476

Purpose

3XFlag::mNeptune::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::mNeptune::Myc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP758-1

Plasmid

#99494

Purpose

TEV::eGFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC156-21XUAS-B2RT>-dSTOP-B2RT>-myr::RFP

Plasmid

#32135

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B2RT> STOP STOP B2RT>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB35

Plasmid

#72866

Purpose

template base vector for N-terminal tagging, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for N-terminal tags, Puro

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB36

Plasmid

#72861

Purpose

template base vector for N-terminal tagging, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for introduction of tags

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA

Plasmid

#194723

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1 (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZHY500

Plasmid

#36186

Depositor

Daniel Voytas

Article

Zhang et al Plant Physiol. 2012 Nov 2.

Insert

FokI homodimeric Nuclease domain

Use

Tags

ACV5

Expression

Yeast

Mutation

delta152,+63 truncation to TAL backbone

Promoter

TEV

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1761 - 1800 of 15192 results

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