We narrowed to 15,323 results for: pET
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Plasmid#73418PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.DepositorInsertPromoter 3F2 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterP3F2 (orthogonal T7-lac variant)Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RFP-RGG
Plasmid#124944PurposeExoression of RGG-RFP-RGGDepositorInsertRGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsHis and mCherryExpressionBacterialAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAR69_STRI_m(KS_MAT)_H8_pET22b
Plasmid#122849PurposeExpresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInsertm(KS_MAT) (Fasn Mouse)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM-30-GST-Dmp68_B
Plasmid#145962PurposeBacterial Expression of Dmp68DepositorInsertDmp68 (Rm62 Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETCON-IgG1/design_50
Plasmid#30301DepositorInsertIgG Fc Designed Binder IgG1
ExpressionYeastAvailable SinceJune 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-A762V
Plasmid#174795PurposeBacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBP-pET-RBS
Plasmid#72981PurposePET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)DepositorInsertpET RBS derived from pET vectors
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP2_rpoA-U
Plasmid#190956PurposeTest the U-to-C editing activity of KP2 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP2
TagsHisExpressionBacterialPromoterT7Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP3_rpoA-U
Plasmid#190960PurposeTest the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP3
TagsHisExpressionBacterialPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002 DA-MBP
Plasmid#102832PurposeBacterial expression of unreactive version of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002 DA-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationReactive aspartic acid has been mutated to an ala…PromoterT7Available SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET12a-KIo18/08 C214S
Plasmid#47099DepositorAvailable SinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p42)
Plasmid#135941PurposeExpresses AUF1p42 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
TagsFlag2 and His6ExpressionBacterialPromoterT7Available SinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHDY*in_PpetE:BCD2:mVenus
Plasmid#133970PurposeHeterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.DepositorInsertmVenus
UseSynthetic BiologyExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-trAtCCD8(pYL736)
Plasmid#178287PurposeExpresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from ArabidopsisDepositorInserttrAtCCD8
ExpressionBacterialPromoterT7Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1-TelR15-YPet
Plasmid#49646PurposeTALEYellow-Telomere for In Vitro TranslationDepositorInsertTelR15
UseIn vitro translationTagsYPetAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP1-His
Plasmid#67869Purposebacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tagDepositorInsertPRORP1 (PRORP1 Mustard Weed)
TagsHisExpressionBacterialMutationResidues 36-572 (see comments)PromoterT7Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-HMGS
Plasmid#108945Purposeheterologous expression of HMGS of E. faecalis in E. coliDepositorInsertHMG-CoA synthase (HMGS)
TagsHis6-TagExpressionBacterialMutationA110GAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaR-Plux
Plasmid#47659PurposePesaR-P promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorInsertPesaR-P
UseSynthetic BiologyExpressionBacterialMutationAdditional esa box downstream of the original esa…PromoterPesaR-PAvailable SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET32a-His-FADD
Plasmid#78803PurposeTo purify His-tagged recombinant FADD proteinDepositorInsertGIG3 (FADD Human)
Tags6x His, S-Tag, Thrombin cleavage site, and TrxA (…ExpressionBacterialPromoterT7Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ClbN-NHis
Plasmid#51497PurposeNRPS module containing CATE domains with N-terminal 6His tagDepositorInsertClbN
Tags6x His tag and T7 tagExpressionBacterialMutationcontains aa30-1094 of NP_754347.1PromoterT7Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1-RRM12CCtoSS
Plasmid#89155PurposeRecombinant expression of PTBP1-RRM12 C250S,C251S in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
Tagshexa HisExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-ZZ(1700-1751)
Plasmid#99334PurposeBacterial expression of mouse CBP ZZ domainDepositorAvailable SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
ymScarletI-YPET pDRF1-GW
Plasmid#168063PurposeFRET fusion between ymScarletI and YPET for use in yeastDepositorInsertymScarletI-YPET
ExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Cdc12-Cdc11
Plasmid#131162PurposeExpression of Septin rodsDepositorInsert6his-Cdc12 + Cdc11
UseBicistronicTagsCdc11: S-Tag and Cdc12: 6hisExpressionBacterialPromoterT7Available SinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-2
Plasmid#172739PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (72R)
Plasmid#62082PurposeExpression of human p53 (1-73) (72R) in e. coliDepositorInsertp53 (TP53 Human)
TagsHIsExpressionBacterialMutationcontains TAD residues 1-73; 72RPromoterT7Available SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET41s-GST-ATF6a-AD
Plasmid#64774Purposebacterial expression of GST-tagged ATF6 (1-150)DepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-239
Plasmid#74609PurposeExpresses Hook domain and first coiled coil of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239 (HOOK3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28 mSmo-Cterm
Plasmid#58703Purposeused for anti-mouse Smoothened antibody production (for affinity purification)DepositorAvailable SinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET - FnR062.Y78W
Plasmid#84802Purposebacterial expression of FnR.0.6.2-Y78W proteinDepositorInsertFnR.0.6.2-Y78W
Tags6xHisExpressionBacterialMutationY78WAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET - FnR062.Y78D
Plasmid#84786Purposebacterial expression of FnR.0.6.2-Y78D proteinDepositorInsertFnR.0.6.2-Y78D
Tags6xHisExpressionBacterialMutationY78DAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET - FnR062.Y78F
Plasmid#84788Purposebacterial expression of FnR.0.6.2-Y78F proteinDepositorInsertFnR.0.6.2-Y78F
Tags6xHisExpressionBacterialMutationY78FAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET - FnR062.Y78G
Plasmid#84789Purposebacterial expression of FnR.0.6.2-Y78G proteinDepositorInsertFnR.0.6.2-Y78G
Tags6xHisExpressionBacterialMutationY78GAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET - FnR062.Y78H
Plasmid#84790Purposebacterial expression of FnR.0.6.2-Y78H proteinDepositorInsertFnR.0.6.2-Y78H
Tags6xHisExpressionBacterialMutationY78HAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET - FnR062.Y78I
Plasmid#84791Purposebacterial expression of FnR.0.6.2-Y78I proteinDepositorInsertFnR.0.6.2-Y78I
Tags6xHisExpressionBacterialMutationY78IAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET - FnR062.Y78P
Plasmid#84796Purposebacterial expression of FnR.0.6.2-Y78P proteinDepositorInsertFnR.0.6.2-Y78P
Tags6xHisExpressionBacterialMutationY78PAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET - FnR062.Y78R
Plasmid#84798Purposebacterial expression of FnR.0.6.2-Y78R proteinDepositorInsertFnR.0.6.2-Y78R
Tags6xHisExpressionBacterialMutationY78RAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET - FnR062.Y78S
Plasmid#84799Purposebacterial expression of FnR.0.6.2-Y78S proteinDepositorInsertFnR.0.6.2-Y78S
Tags6xHisExpressionBacterialMutationY78SAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET - FnR062.Y78
Plasmid#84783Purposebacterial expression of FnR.0.6.2-Y78 proteinDepositorInsertFnR.0.6.2-Y78
Tags6xHisExpressionBacterialAvailable SinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET29b-Ava3858-3857
Plasmid#45744DepositorInsertAva3858-3857
ExpressionBacterialPromoterT7Available SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 S400D
Plasmid#16156DepositorAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28b+R3(R112C/T180C)
Plasmid#108195PurposeExpresses PdR-PCNA3(R112C/T180C) fusion protein in E. coliDepositorInsertPdR-PCNA3(R112C/T180C) fusion protein
TagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b Me31B-3Myc
Plasmid#113001PurposeFor protein expression and purification of Myc-tagged full-length Drosophila me31BDepositorAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETO18G-H7C/R86C
Plasmid#27403DepositorInsertleuT
TagsHisX8 and ThrombinExpressionBacterialMutationChanges His 7 to Cys and Arg 86 to CysAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-SNAP-HCA_sensor
Plasmid#127181PurposeBacterial expression of the fusion protein used for sulfa-drugs sensingDepositorInsertSNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
TagsHisTag and StrepTagExpressionBacterialPromoterT7Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 S142A
Plasmid#16153DepositorAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 G399S
Plasmid#16158DepositorAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET21a-hAIM2-deltaPYD
Plasmid#51522PurposeExpress His6-tagged HIN domain of hAIM2 in bacteriaDepositorInsertAIM2 (AIM2 Human)
TagsHis6 and T7ExpressionBacterialMutationdeletion of pyrin domainPromoterlac operatorAvailable SinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET29NK-LGK/mGFPmut3
Plasmid#81168PurposeGFP fusion to levoglucosan kinaseDepositorInsertLevoglucosan kinase wild-type
TagsHis6x and mGFPmut3ExpressionBacterialPromoterT7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-G51PEKN
Plasmid#118582PurposeBacterial expression of E.coli DHFR G51PEKN mutantDepositorInsertDHFR
ExpressionBacterialMutationG51 mutated to P51; Glu-Lys-Asn residues inserted…PromoterT7Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only