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pDY0216 PASTEv1 PE2-P2A-Bxb1

Plasmid

#179104

Purpose

PASTEv1 PE2-P2A-Bxb1

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-PE2-P2A-Bxb1

Expression

Mammalian

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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PEA1-GFP

Plasmid

#171993

Purpose

Delivers all prime editing nickase components in a single, GFP selectable plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Expression

Mammalian

Promoter

CMV for Cas9, U6 for gRNAs

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pNIC28-MacroGreen

Plasmid

#160665

Purpose

Expresses GFP fusion of a multisite mutant of the Af1521 protein

Depositor

Herwig Schüler

Article

Garcia-Saura et al Commun Biol. 2021 Jul 28;4(1):919. doi:

Insert

Af1521 modified synthetic construct eGFP fusion

Tags

eGFP and hexahistidine_TEV recognition

Expression

Bacterial

Promoter

LacI

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.Luciferase.IRES.GFP

Plasmid

#169308

Purpose

Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-EGFP

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gαi*-BERKY3

Plasmid

#158198

Purpose

BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-(empty)-TAG

Plasmid

#138209

Purpose

To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

Depositor

Neal Devaraj

Article

Busby et al Methods Enzymol. 2020;641:373-399. doi:

Type

Empty backbone

Use

T7 transcription

Tags

TAG sequence

Expression

Mammalian

Promoter

CMV Promoter, T7 Promoter

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57.1 Lysosomal-METRIQ

Plasmid

#135401

Purpose

Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity

Depositor

Eisuke Itakura

Article

Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

Insert

DNAse II alpha (DNASE2 Human)

Use

Lentiviral

Tags

T2A, mCherry, and sfGFP

Expression

Mammalian

Mutation

Single-nucleotide polymorphism C432T

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-GFP-OSBP

Plasmid

#134659

Purpose

Flag and GFP-tagged OSBP

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

OSBP (OSBP Human)

Use

Lentiviral

Tags

FLAG and GFP

Expression

Mammalian

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pemiRFP670-tubulin

Plasmid

#136573

Purpose

Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP670-tubulin

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-Giantin

Plasmid

#129630

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

mTurquoise2-Giantin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

Plasmid

#127354

Purpose

gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, V5, and mVenus

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSGAb-spe

Plasmid

#122000

Purpose

A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Insert

none

Use

CRISPR

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

Plasmid

#112925

Purpose

Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6 promoter, gRNA-SAM expression cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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tsPurple chromoprotein

Plasmid

#117848

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, tsPurple

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_G3BP1

Plasmid

#127106

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

G3BP1 (G3BP1 Human)

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

Plasmid

#117484

Purpose

Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyCatcher002-6aa-sfCherry2(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-eGFP-W

Plasmid

#126686

Purpose

Constitutive, ubiquitous GFP expression.

Depositor

Darrell Kotton

Article

Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

Insert

eGFP

Use

Lentiviral

Promoter

EF1aL

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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LentiV_Cas9_Blast

Plasmid

#125592

Purpose

Lentiviral expression of spCas9 with blasticidin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-mNeonGreen

Plasmid

#99133

Purpose

An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV1

Insert

fDIO-mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RAD50

Plasmid

#116784

Purpose

Lentiviral expression of RAD50

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RAD50 (RAD50 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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