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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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159,589 results

Showing: 9221 - 9240 of 159589 results

  1. BNLS

    Plasmid
    #50836
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP B
    Use
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50836-plasmid-map-sequence-id-76280

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  12. TFORF2873

    Plasmid
    #143648
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ELF1 (ELF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143648-plasmid-map-sequence-id-400475

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  14. CXCR2-DuET

    Plasmid
    #213219
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR2 (CXCR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213219-plasmid-map-sequence-id-430781

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  18. pPROEX-TwStrp-FTO

    Plasmid
    #190265
    Purpose
    Expresses FTO in bacterial cells, with two purification tags: His and Twin-Streptag.
    Depositor
    Samie Jaffrey
    Insert
    FTO (FTO Human)
    Use
    Tags
    6xHis and Twin-Streptag
    Expression
    Bacterial
    Mutation
    Promoter
    Trc
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    190265-plasmid-map-sequence-id-371437

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  20. SERPINE2-bio-His

    Plasmid
    #53416
    Purpose
    Expresses full-length Glia-derived nexin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SERPINE2 (SERPINE2 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53416-plasmid-map-sequence-id-83200

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Showing: 9221 - 9240 of 159589 results