Addgene

We narrowed to 13,557 results for: TIM

Showing: 2301 - 2350 of 13557 results

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YN2_1_LT84_RPL13A

Plasmid

#177712

Purpose

Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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YeNL-Actin_pcDNA3

Plasmid

#89521

Purpose

Yellow color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pNB777

Plasmid

#60153

Purpose

Red click beetle luciferase (Promega) driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBR

Use

Tags

Expression

Yeast

Mutation

Promoter

LEU1pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-L4-pTF-L1R

Plasmid

#45954

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Insert

pTF promoter

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

TET optimized

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pME-QF2w-SV40pA

Plasmid

#155117

Purpose

Middle entry vector containing QF2w

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QF2w

Use

Gateway middle entry vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2

Plasmid

#124176

Purpose

piggyBac-based Dox-inducible expression (moderate)

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
tubulin-YeNL_pcDNA3

Plasmid

#89519

Purpose

Yellow color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Use

Tags

b-tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pYFP-lacY-1

Plasmid

#140138

Purpose

Evaluating the impact of feedback on information transmission of a genetic sensor.

Depositor

Drew Tack

Article

Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

Insert

lacY (lacY )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHFX-cas3UD

Plasmid

#174385

Purpose

Plasmids for gene knockout were constructed based on suicide plasmid pHFX

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

Cas3UD

Use

Archaeal gene knockout vector for haloferax medit…

Tags

Expression

Mutation

Promoter

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/PH* (W495L) uGFP

Plasmid

#14564

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

PH* (W495L)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/CR* (C529S) uGFP

Plasmid

#14560

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/CR* (C529S)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
OeNL-H2B_pcDNA3

Plasmid

#89527

Purpose

Orange color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

histone 2B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSox2-bd::FP

Plasmid

#34703

Depositor

Hollis Cline

Article

Bestman et al J Comp Neurol. 2012 Feb 1;520(2):401-33.

Insert

eGFP

Use

Xenopus expression

Tags

165-bp minimal FGF4 promoter, 6x Sox2/Oct3-4 tran…

Expression

Mutation

Promoter

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-crRNA1

Plasmid

#123639

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

crRNA scaffold 1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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RV-Syn-Dio-GFP

Plasmid

#87673

Purpose

retroviral vector encoding cre dependent expression of GFP

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

eGFP

Use

Retroviral

Tags

Expression

Mutation

Promoter

Synapsin

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8ENdeI-cHAIAA7-spo5DSR

Plasmid

#49873

Purpose

for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

IAA17 degron tag

Expression

Mutation

Promoter

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1.linker_KO

Plasmid

#164688

Purpose

For CRISPR knockout of miR-144~451 linker region by lentiviral delivery of Cas9 and linker gRNA

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

miR-144~451 linker CRISPR KO gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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KA1130_pPB-TREtight-cHA-IRESH2BmCherry

Plasmid

#124173

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER-retro-puro-shNup133-2

Plasmid

#87333

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
OeNL-Actin_pcDNA3

Plasmid

#89524

Purpose

Orange color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS030: ratiometric pHluorin (endoplasmic reticulum)

Plasmid

#163054

Purpose

ratiometric pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB774

Plasmid

#60114

Purpose

Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

GrLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Mutation

Promoter

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ414 - DANCER-3

Plasmid

#136387

Purpose

Expresses DANCER-3 dynamic protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

DANCER-3

Use

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V39L-V54I

Promoter

T7 promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET FLII32Pglu-2.2m

Plasmid

#13566

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A P32S-ECFP-D33R

Use

Tags

6xHis and Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCBB-lacZa

Plasmid

#32563

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

LacZ-alpha

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Mutation

Promoter

Consitutive lac

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
KA2706_eSpCas9-PGKPacdtkCh

Plasmid

#124204

Purpose

Expression of eSpCas9(1.1) and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYFP-x-1

Plasmid

#140142

Purpose

Evaluating information transmission of a genetic sensor.

Depositor

Drew Tack

Article

Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

Insert

lacY (lacY )

Use

Tags

Expression

Bacterial

Mutation

D37, I38, and N39 are changed to nonsense (stop) …

Promoter

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ158

Plasmid

#69307

Purpose

Gateway entry vector with hSpCas9 (Human codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9 (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Expression

Mutation

NLS fusion

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSox2-bd_mFGF_eGFP

Plasmid

#34704

Depositor

Hollis Cline

Article

Bestman et al J Comp Neurol. 2012 Feb 1;520(2):401-33.

Insert

eGFP

Use

Xenopus expression

Tags

165-bp minimal FGF4 promoter and 6x Sox2/Oct3-4 t…

Expression

Mutation

Promoter

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-PCNA-19-NLS-4

Plasmid

#54222

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

Insert

PCNA-19 NLS (PCNA Human)

Use

Tags

SV40NLS and mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
B3rec.zf1

Plasmid

#108354

Purpose

Zebrafish codon optimized B3 recombinase that acts on blown-out sites

Depositor

Harold Burgess

Article

Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

Insert

B3 recombinase

Use

Synthetic Biology

Tags

Expression

Mutation

Zebrafish codon optimized

Promoter

lac

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB763

Plasmid

#60158

Purpose

Firefly luciferase (Promega) driven by LYS9 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Use

Tags

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LYS9pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK33/intein-Cas9_S219-3XFLAG

Plasmid

#133782

Purpose

Expresses human codon-optimized intein-Cas9

Depositor

Norbert Perrimon

Article

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Insert

Human codon-optimized intein-Cas9

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RV-Cag-Dio-mTdt

Plasmid

#87665

Purpose

retroviral vector encoding cre dependent expression of membrane Tdtomato

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Mem-Tdtomato

Use

Retroviral

Tags

Expression

Mutation

Promoter

CAG

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-XIAPmut

Plasmid

#8341

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAPmut (XIAP Human)

Use

Tags

GST

Expression

Bacterial

Mutation

H467A

Promoter

Available since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only
pFH84

Plasmid

#128195

Purpose

Level1 ZmUBIp::A3A-PBE (wheat codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::A3A-PBE (wheat codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH82

Plasmid

#128193

Purpose

Level1 ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH93

Plasmid

#128196

Purpose

Level1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoEmerald-SpHis5

Plasmid

#44851

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Emerald with SpHis5 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEmerald

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
RV-Cag-Dio-kikume

Plasmid

#87663

Purpose

retroviral vector encoding cre-dependent expression of kikume

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

kikume

Use

Retroviral

Tags

Expression

Mutation

Promoter

CAG

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB761

Plasmid

#60156

Purpose

Firefly luciferase (Promega) driven by ADE17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Use

Tags

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

ADE17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB775

Plasmid

#60115

Purpose

Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

YeLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Mutation

Promoter

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB776

Plasmid

#60117

Purpose

Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

RdLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Mutation

Promoter

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRmHa3-dKDM4A-HA2FL2

Plasmid

#20232

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Use

Tags

FLAG2 and HA2

Expression

Insect

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
R300TA

Plasmid

#80411

Purpose

human cTNT minigene with genomic segment containing alternative exon 5 with CELF1 binding site mutated (CTGs->CAGs)

Depositor

Thomas Cooper

Article

Philips et al Science. 1998 May 1;280(5364):737-41.

Insert

TNNT2 (TNNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

CTGs downstream of alternative exon mutated to CA…

Promoter

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-PCNA-19-SV40NLS-4

Plasmid

#54223

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

Insert

PCNA-19 SV40NLS (PCNA Human)

Use

Tags

3xSV40NLS and mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAW8ENdeI-cyEGFP-spo5DSR

Plasmid

#49867

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Expression

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoMaxGFP-Kan

Plasmid

#44907

Purpose

Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoMaxGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
tdTurboRFP-pBAD

Plasmid

#54857

Purpose

Localization: Protein Expression Vector , Excitation: 553, Emission: 576

Depositor

Dmitriy Chudakov, Michael Davidson, Dorus Gadella

Article

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Use

Tags

6xHis and tdTurboRFP

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBacPAK-HFT-dKDM4A

Plasmid

#20233

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Use

Tags

FLAG, His, and TEV

Expression

Insect

Mutation

Promoter

Available since

March 17, 2009

Availability

Academic Institutions and Nonprofits only

Showing: 2301 - 2350 of 13557 results

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