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20,361 results

Showing: 9401 - 9420 of 20361 results

  4. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid
    #84240
    Purpose
    Expresses GA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sp dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84240-plasmid-map-sequence-id-160812

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  5. Grp1-PH pEGFP-C1

    Plasmid
    #71378
    Purpose
    For analysis of PH domain localization in mammalian cells
    Depositor
    Mark Lemmon
    Publication
    Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Grp1-PH (Cyth3 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Pleckstrin homology (PH) domain; aa 247-399
    Promoter
    CMV
    Available since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71378-plasmid-map-sequence-id-422685

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  7. p5E-gfap

    Plasmid
    #75024
    Purpose
    Can be used to drive expression in zebrafish astrocytes.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    glial fibrillary acidic protein promoter (gfap Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    gfap
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75024-plasmid-map-sequence-id-450679

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  11. CAG sHRPa-NRX

    Plasmid
    #73143
    Purpose
    Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sHRPa-NRX
    Tags
    2x HA tag and Flag
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    73143-plasmid-map-sequence-id-134511

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  16. alpha catenin conformation sensor

    Plasmid
    #71709
    Purpose
    This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.
    Depositor
    Deborah Leckband
    Publication
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha-catenin FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71709-plasmid-map-sequence-id-142365

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  19. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Publication
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    pBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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Showing: 9401 - 9420 of 20361 results