Addgene

We narrowed to 31,148 results for: cmv

Showing: 1881 - 1920 of 31148 results

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cmv 16 E7 del DLYC

Plasmid

#13687

Depositor

Karl Munger

Article

Gonzalez et al J Virol. 2001 Aug . 75(16):7583-91.

Insert

HPV 16 E7 del DLYC (E7 Human)

Use

Tags

Expression

Mammalian

Mutation

del aa 21-24

Promoter

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-Y-RAN-RFP6

Plasmid

#106424

Purpose

Y-RAN-RFP6 (RANbody RFP6::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-RFP6

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV:GFP(FRT)lacZ

Plasmid

#31124

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

GFP(FRT)lacZ

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OgNLuc_SV40:PuroR

Plasmid

#183038

Purpose

Expresses the FLAG-tagged OgNLuc (Orange LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pR26-CMVconst-mBMP7

Plasmid

#127375

Purpose

Allows for constitutive BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

Depositor

Lance Miller

Article

Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

Insert

BMP7 (Bmp7 Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

CMV enhancer/promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::ZeoR-bGHpA

Plasmid

#62441

Purpose

MXS_chaining vector with CMV::ZeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Zeocin with CMV Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-pOTC-3H-GFP

Plasmid

#205725

Purpose

Expression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

pOTC-3H-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CASANOVA

Plasmid

#113036

Purpose

AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-H-RAN-GFP1

Plasmid

#106418

Purpose

H-RAN-GFP1 (RANbody GFP1::Sm-HAtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

H-RAN-GFP1

Use

Tags

HA, His, and Multiple HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::HygroR-bGHpA

Plasmid

#62435

Purpose

MXS_chaining vector with CMV::HygroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with CMV Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-AAVS1-sg

Plasmid

#194716

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hAAVS1 locus

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1 (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(ΔL1)

Plasmid

#80049

Purpose

Expression of EPN-01 mutated in TSG101 binding site.

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaPTAP)

Use

Tags

Myc

Expression

Mammalian

Mutation

PTAP10AAAA

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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4xHRE_minSV40-sfGFP-CMV_dsRed

Plasmid

#117400

Purpose

sfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_APOBEC1-UdgX-nCas9

Plasmid

#163531

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

APOBEC1-UdgX-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-iRFP

Plasmid

#64887

Purpose

Marking retinal neurons with infrared fluorescent proteins (iRFP) to preserve photoresponsiveness

Depositor

Tomomi Ichinose

Article

Fyk-Kolodziej et al Biotechniques. 2014 Nov 1;57(5):245-53.

Insert

iRFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3B

Plasmid

#193642

Purpose

Expression of base editor dCas12a-A3B in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3B, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3G

Plasmid

#193643

Purpose

Expression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3G, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-RFP4

Plasmid

#106410

Purpose

P-RAN-RFP4 (RANbody RFP4::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-RFP4

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-NHERF1-PDZ1iP

Plasmid

#28295

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains aa 1-148 (deletion of PDZ2 and EB domain…

Promoter

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-luc-miR21(P)

Plasmid

#20382

Depositor

Bryan Cullen

Article

Zeng et al RNA. 2003 Jan . 9(1):112-23.

Insert

mir21(P) targets

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-C71,72,105A

Plasmid

#58396

Purpose

Expressed murine IFITM3-C71,72,105A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Cysteines 71, 72, and 105 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pBK-CMV-mUBP43

Plasmid

#12459

Depositor

Dong-Er Zhang

Article

Liu et al Mol Cell Biol. 1999 Apr . 19(4):3029-38.

Insert

UBP43 (Usp18 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
U6-spTRE3G-CMV-mTagBFP2

Plasmid

#102854

Purpose

A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

Depositor

Michael Lin

Article

Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

Insert

sp-TRE3G-sgRNA; mTagBFP2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/WT M87

Plasmid

#89322

Purpose

expression of WT M87 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 bp deleted

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-luc-random

Plasmid

#20877

Depositor

Bryan Cullen

Article

Zeng et al RNA. 2003 Jan . 9(1):112-23.

Insert

random sequence targets

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pFLAG-CMV2-CARP1

Plasmid

#16014

Depositor

Wafik El-Deiry

Article

McDonald et al Proc Natl Acad Sci U S A. 2004 Apr 20. 1

Insert

CARP1 (RNF34 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-Tag 3A/WT myc-M1

Plasmid

#87719

Purpose

Expresses WT myc-M1 spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
miniCMV-EGFP-tDeg

Plasmid

#185400

Purpose

EGFP-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

EGFP-tDeg

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-DNAJC5 (WT)-HaloTag

Plasmid

#205710

Purpose

Mammalian expression of DNAJC5 (WT)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-CMV-GFP-W

Plasmid

#196046

Purpose

lentiviral expression of the green fluorescent protein driven by cytomegalovirus virus promoter

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mutation

Promoter

CMV

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mGold-Actin-C-18

Plasmid

#158009

Purpose

Visualization of actin in mammalian cells using mGold

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

Actin (ACTB Human)

Use

Tags

mGold

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-OptoFGFR1-D387A

Plasmid

#59778

Purpose

Expresses optoFGFR1 with mutation in PHR to suppress homointeraction, thus cannot be activated by light

Depositor

Won Do Heo

Article

Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

Insert

OptoFGFR1-D387A (FGFR1 Human, Mustard Weed)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mNoc-3X FLAG

Plasmid

#90465

Purpose

expression of Mouse Nocturnin with 3x Flag tag

Depositor

Carla Green

Insert

Nocturnin (Noct Mouse)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mA1-BE3

Plasmid

#113421

Purpose

Expresses mA1-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

mA1-BE3 (Apobec1 S. pyogenes and Bacteriophage PBS2, Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-TFE3pA

Plasmid

#120201

Purpose

Human TFE3 poly(A) site reporter

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

TFE3 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-mEGFP-PKCg-C1

Plasmid

#112266

Purpose

ITRACKgamma Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase c gamma (Prkcg Mouse)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LCMV:ECFP(loxP)MCS

Plasmid

#31376

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

ECFP(loxP)MCS

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-MBP-yATOM_Ras

Plasmid

#209709

Purpose

Mammalian expression of y-ATOM biosensor for HRas

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_Ras

Use

Tags

MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV HDM2(NES-)

Plasmid

#12087

Depositor

Tyler Jacks

Article

Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

Insert

HDM2 (MDM2 Human)

Use

Tags

Expression

Mammalian

Mutation

Nuclear-export sequence mutant (NES-): L205A, I20…

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
LentiGuide-BC-CMV-Puro

Plasmid

#127169

Purpose

PCR template for CMV-puro resistance cassette

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

None

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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