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Plasmid#207148PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI1 (GNAI1 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)KPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX302 IL10RA-V5 puro
Plasmid#47552PurposeLentiviral expression of V5-tagged IL10RA in mammalian cellsDepositorInsertIL10RA (IL10RA Human)
UseLentiviralTagsV5ExpressionMammalianMutationNonePromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEM-PH-citrine
Plasmid#131406PurposePurpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrineDepositorInsertPH
UseIn vitro transcriptionTagscitrine yellow fluorescent proteinExpressionMutationPromoterT7Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
DTECT - Plasmid for Standard Curve
Plasmid#139333PurposeThis plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECTDepositorInsertDTECT ligation product
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-IV
Plasmid#53440PurposeDerivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IVDepositorInsertagrBD locus (with D29Y mutation in agrD)
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationD to Y mutation of residue 29 of the agrD gene (c…PromoterT7-lacAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBG Jip3 (nterm)
Plasmid#58942Purposeinteracts with MAPK signaling moduleDepositorInsertmitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
UseTagsGSTExpressionMammalianMutationdeleted aa 271-1817PromoterAvailable sinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Bov.hsc70.RSET.a1-554.wt(NarI)
Plasmid#12533DepositorInsertBovine 70 kDa heat shock cognate protein fragment amino acids 1-554
UseTagsExpressionBacterialMutationdeleted carboxy-terminal residues 555-650 of bovi…PromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 T538N
Plasmid#60537PurposeDonor vector for human Pak5 T538N mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationT538NPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 S573N
Plasmid#60538PurposeDonor vector for human Pak5 S573N mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationS573NPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-PC-2-CTS
Plasmid#86068PurposeA bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.DepositorInsertPolycystin-2 (Pkd2 Human)
UseTagsGSTExpressionBacterialMutationcontains the region from 1-15PromotertacAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-GINIP-Nluc W139A
Plasmid#223557PurposePlasmid expressing GINIP-Nluc W139A in mammalian cellsDepositorInserthuman GINIP W139A - linker(GGGS) - Nluc
UseTags3xFLAGExpressionMammalianMutationW139A mutation in GINIP sequencePromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML B-box1 WT
Plasmid#185632Purposeexpression of GST-tagged wild-type PML B-box1 domain in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationnonePromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML B-box1 C4C4
Plasmid#185640Purposeexpression of GST-tagged mutant PML B-box1 H155C/H161C domain in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationH155C H161CPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML B-box1 W157E
Plasmid#185641Purposeexpression of GST-tagged mutant PML B-box1 W157E domain in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationW157EPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML B-box1 F158E
Plasmid#185642Purposeexpression of GST-tagged mutant PML B-box1 F158E domain in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationF158EPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-MAFG-V5-miniTurbo
Plasmid#207818PurposeC-terminal tagged MAFG for miniTurbo proximity experimentsDepositorInsertMAFG-V5-miniTurbo
UseLentiviralTagsV5-miniTurboExpressionMutationPromoterUBCAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-MAFG
Plasmid#207817PurposeN-terminal tagged MAFG for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-MAFG
UseLentiviralTagsminiTurbo-V5ExpressionMutationPromoterUBCAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-ZNF746
Plasmid#207819PurposeN-terminal tagged ZNF746 for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-ZNF746 (ZNF746 Human)
UseLentiviralTagsminiTurbo-V5ExpressionMutationPromoterUBCAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-NS-A
Plasmid#207823PurposeDual expression of Cas9 and non-specific sgRNADepositorInsertnon-specific sgRNA
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterEF-1a; U6Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-NS-B
Plasmid#207824PurposeDual expression of Cas9 and non-specific sgRNADepositorInsertnon-specific sgRNA
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterEF-1a; U6Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only