We narrowed to 2,701 results for: LRE
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Plasmid#74962PurposeCas9 + sgATXN1L-1 with blasticidin selectionDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only
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PXPR007 sgATXN1L-2
Plasmid#74963PurposeCas9 + sgATXN1L-2 with blasticidin selectionDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-hTSC1-T2A-Bsd
Plasmid#193199PurposeLentiviral vector expressing human TSC1 cDNA (driven by EFS promoter) and a blasticidin resistance markerDepositorAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE
Plasmid#87919PurposeHIV-1 SIN lentiviral transfer vectorDepositorInsertsgHTT1, shGFP2, sgCas9, cherry
UseLentiviralPromoterH1, U6, 7SK, PGKAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 D122A HA pCAGGS
Plasmid#206097Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1DepositorAvailable SinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA D122A pcDNA3
Plasmid#206108Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-K298M-Y419F-Y530F
Plasmid#140320PurposeExpresses HA-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
Tags2X HAExpressionMammalianMutationK298M-Y419F-Y530FPromoterCMVAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-IgM
Plasmid#187013PurposeExpression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgMDepositorInsertHuman anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM
UseLentiviralAvailable SinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Ascl1.MIRT124_x4 Xma_lost.WPRE
Plasmid#67290PurposeInduce human iN conversion from fibroblastDepositorAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-NLS-BirA-2a-mCherry
Plasmid#79886PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
Tags3x HAExpressionBacterialPromoterUbiquitinAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-MCS-NLS-BirA-2A-mCherry_Ras
Plasmid#80059PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-zic2a-cytoBirA-2A-mCherry_Ras
Plasmid#80064PurposeZic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterZic2a enhancer driving c-fos promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli2 P1-4A
Plasmid#51252PurposeEncodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to AlaDepositorInsertGli2 (Gli2 Mouse)
UseFlpin systemTags3xHA-TEVExpressionMammalianMutationamino acids 789,805,817,848 mutated to AlaPromoterEF1aAvailable SinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNotch1#1/Cre
Plasmid#193223PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 geneDepositorAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNotch2#2/Cre
Plasmid#193226PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 geneDepositorAvailable SinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)
Plasmid#174336PurposeThe ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2DepositorInserthuman Orai1 (ORAI1 Human)
UseRetroviralTagspHluorin inserted in the extracellular loop 2 of …ExpressionMammalianPromoterCMVAvailable SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AOI-WT-Cas9-sg-mouse Suz12-F2-GFP
Plasmid#91882PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12DepositorAvailable SinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO-3XFLAG Halo delta 1-117 nsp1
Plasmid#175423PurposeExpresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 delta 1-117 nsp1
Tags3XFLAG HaloExpressionMammalianMutationnsp1 C-terminusPromoterCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo delta 118-180 nsp1
Plasmid#175424PurposeExpresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 delta 118-180 nsp1
Tags3XFLAG HaloExpressionMammalianMutationN-terminus of nsp1PromoterCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S32
Plasmid#140308PurposeExpresses HA-tagged human Src-S32 in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X HAExpressionMammalianMutationcontains only the SH3 and SH2 domainsPromoterCMVAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-HRV3C/D614G
Plasmid#164568PurposeSARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)DepositorInsertSpike (S-HRV3C-D614G variant)
Tags2X Strep-Tag II and 8X His tagExpressionMammalianMutationD614GAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO-3XFLAG Halo R124A/K125A nsp1
Plasmid#175417PurposeExpresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 R124A/K125A nsp1
Tags3XFLAG HaloExpressionMammalianMutationR124A and K125APromoterCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA1 Q1779Stop
Plasmid#139331PurposePlasmid expressing a sgRNA to introduce BRCA1 Q1779Stop using base editingDepositorInsertsgRNA to insert BRCA1 Q1779Stop using base editing
ExpressionMammalianPromoterU6Available SinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA1 T922I
Plasmid#139328PurposePlasmid expressing a sgRNA to introduce BRCA1 T922I using base editingDepositorInsertsgRNA to insert BRCA1 T922I using base editing
ExpressionMammalianPromoterU6Available SinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shDyn2
Plasmid#132712PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn geneDepositorAvailable SinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-GCN4-dCEN-INCENP
Plasmid#108510Purposeexpression of vsv-GCN4-dCEN-INCENPDepositorInsertGCN4-dCEN-INCENP (INCENP Human)
TagsVSVExpressionMammalianMutationreplace CEN box for GCN4 dimerazation domainPromoterCMVAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-BASIC- ARHGAP29 Mutant PROMOTER
Plasmid#104156Purposeluciferase reporter with ARHGAP29 mutant promoterDepositorInsertARHGAP29 promoter (ARHGAP29 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationdelete two TEAD binding sitesPromoterARHGAP29Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S32-KD
Plasmid#140310PurposeExpresses HA-tagged human Src-S32-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X HAExpressionMammalianMutationContains only the SH3, SH2, and KD regionsPromoterCMVAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgATXN1L-1
Plasmid#74970PurposesgATXN1L-1, puromycin selectionDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV8-hSyn-flex-miR30-eGFP-shNts
Plasmid#132717PurposeEncodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Nts gene which encodes neurotensinDepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgCIC-2
Plasmid#74967PurposesgCIC-2, puromycin selectionDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST-EGFP-Cx43ML-N1
Plasmid#49860PurposeEncodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tagDepositorInsertConnexin 43 (GJA1 Human)
TagsEGFPExpressionMammalianMutationAll internal Methionines mutated to Leucine. No s…PromoterCMVAvailable SinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli2 P5-6D
Plasmid#51254PurposeEncodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to AspDepositorInsertGli2 (Gli2 Mouse)
UseFlpin systemTags3xHA-TEVExpressionMammalianMutationamino acids 923,956 mutated to AspAvailable SinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA
Plasmid#154779Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertLaG17-SynNotch
TagsLaG17ExpressionMammalianMutation204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-ML-HA
Plasmid#49857PurposeEncodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal HA epitope fusion tagDepositorInsertConnexin 43 (GJA1 Human)
TagsHAExpressionMammalianMutationAll internal Methionines mutated to Leucine. Cont…PromoterCMVAvailable SinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGria3
Plasmid#124869PurposeMutagenesis of Gria3DepositorInsertGria3 (Gria3 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-ATOH7-T2A-PuroR
Plasmid#162343PurposeLentiviral expression of ATOH7 under the control of the TetON promoterDepositorAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shDyn1
Plasmid#132711PurposeEncodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Pdyn geneDepositorAvailable SinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSN67
Plasmid#100124Purposeexpresses putative KLF4 DBD with a 6xHis tagDepositorInsertmCherry-P2A-putative KLF4 DBD with a 6xHis tag (KLF4 Human)
UseLentiviralExpressionMammalianMutationInsert corresponds to the DBD of KLF4Available SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
USPQ-HTT-TALEN1
Plasmid#92243PurposeTALEN targeting upstream of HTT gene (KKR), forms obligate heterodimer with USPQ-HTT-TALEN2DepositorInsertTALEN
UseTALENPromoterCMVAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only