We narrowed to 61,534 results for: promoter
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Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorTypeEmpty backboneTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by
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lentiCRISPRv2 hygro
Plasmid#98291PurposeVariant of lentiCRISPRv2 that confers hygromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralPromoterEF-1aAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-PEmax-P2A-hMLH1dn
Plasmid#174828PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPEmax-P2A-hMLH1dn
TagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-p300
Plasmid#23252DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertp300 (EP300 Human)
Tags6xHisExpressionMammalianPromoterCMVAvailable sinceMarch 10, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FLAG-HA-pcDNA3.1-
Plasmid#52535Purposeenables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteinsDepositorTypeEmpty backboneTagsFLAG-HAExpressionMammalianPromoterCMVAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-OVA
Plasmid#64599PurposeExpression of chicken ovalbumin as model antigen for study of MHC class I and class II presentationDepositorPublicationInsertOvalbumin
ExpressionMammalianPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Clover
Plasmid#40259PurposeGFP with robust performance in protein fusions and useful as FRET donor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlapDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClover GFP
ExpressionMammalianPromoterCMV IEAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-syn-FLEX-jGCaMP7s-WPRE
Plasmid#104491PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switchDepositorHas serviceAAV1, AAV PHP.eB, and AAV RetrogradeInsertjGCaMP7s
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterSynapsinAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-p62
Plasmid#28027PurposeMammalian expression of HA tagged human p62DepositorPublicationAvailable sinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-SARS2-Spike
Plasmid#145032PurposeExpress SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 Spike (S Synthetic)
TagsC9ExpressionMammalianPromoterCMVAvailable sinceApril 15, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pLJC5-Tmem192-3xHA
Plasmid#102930PurposeLentiviral expression of a lysosomal tag (Tmem192-HA)DepositorAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
c-myc promoter (Frag A)
Plasmid#16605DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertc-myc promoter (Frag A) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationFragA (XmnI->KpnI). The KpnI site is at -1194 …Available sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 neo
Plasmid#13425Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.DepositorInsertnon-hairpin 18bp
UseLentiviral and RNAiExpressionMammalianPromoterU6 promoterAvailable sinceDec. 22, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCXWB-EBNA1
Plasmid#37624PurposeNon-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmidsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEBNA-1 (EBNA-1 human herpesvirus 4)
ExpressionMammalianPromoterCAGAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
1037 pGL3 FasL promoter
Plasmid#9028DepositorAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmiRFP670-N1
Plasmid#79987PurposeMonomeric near-infrared fluorescent protein miRFP670 in mammalian plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmiRFP670
ExpressionMammalianPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-HA-Ubiquitin-K6R
Plasmid#121153PurposeMammalian expression of HA tagged ubiquitin K6RDepositorAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMAZe
Plasmid#60877PurposeReporter construct for lineage tracing and mosaic analysis in zebrafish.DepositorInsertshsp70l promoter
Cre
dTomato
UAS promoter
Gal4VP16
UseZebrafish expressionAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-tdTomato-WPRE
Plasmid#51506PurposeCan be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoterDepositorHas serviceAAV5InserttdTomato
UseAAVPromoterphSyn1Available sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-ABE7.10
Plasmid#102919Purposeexpresses ABE7.10 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABE7.10
TagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by