172,688 results

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FH1-MGT#2-mCherry_PGK-H2B-CFP

Plasmid

#164102

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

MGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RLuc

Plasmid

#83282

Purpose

recombinant AAV vector packaging single-stranded Renilla Luciferase under the CAG promoter (includes WPRE element)

Depositor

Mark Kay

Insert

Renilla Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-loxp-DsRed-loxp-eGFP

Plasmid

#141148

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

loxp-DsRed-loxp-eGFP

Use

Lentiviral

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-EGFP-NR2a wt

Plasmid

#45445

Depositor

Andres Barria, Robert Malinow

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR2A (Grin2a Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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CMV500 empty vector

Plasmid

#33348

Depositor

Charles Vinson

Article

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2011

Availability

Academic Institutions and Nonprofits only

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SaABE8e

Plasmid

#138500

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSaCas9

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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GalT-RpHLuorin2

Plasmid

#171719

Purpose

Trans-Golgi expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF_6xHIS_Csx29

Plasmid

#197613

Purpose

For protein purification of Csx29

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

6-HIS tagged Csx29

Expression

Bacterial

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[himar1C9]

Plasmid

#213906

Purpose

Mariner transposon with ATc-inducible himar1C9 transposase

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with cam resistance and ATc-inducible himar1C9 transposase

Expression

Bacterial

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pOpen-RNAse A

Plasmid

#165565

Purpose

Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

RNAse A

Use

Synthetic Biology

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pS6-TGFB2-H8

Plasmid

#128502

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t H8x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (L21-S414) (TGFB2 Human)

Tags

His tag (6x)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pKIKan_idCas9_Chr_center

Plasmid

#149637

Purpose

suicide vector for knock in into the B. burgdorferi chromosome

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-GABARAPL2-GFP

Plasmid

#123113

Purpose

Expresses 3xFLAG-GABARAPL2-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL2

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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Flamindo2

Plasmid

#73938

Purpose

Expresses biosensor for cAMP in mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

Insert

Flamindo2

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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PCS2+Cas9-mSA

Plasmid

#103882

Purpose

Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

CAS9-MSA

Use

CRISPR

Tags

MSA (monomeric streptavidin

Mutation

none

Promoter

SP6

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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ARK2aa

Plasmid

#81013

Purpose

E.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Inserts

MevTo-BBa1002-pTrc-Mkco-aphA
and PMD

Promoter

LacUV5 and Trc promoter

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCRCT

Plasmid

#60621

Purpose

Plasmid encoding iCas9, tracrRNA and crRNAs

Depositor

Huimin Zhao

Article

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNP1723

Plasmid

#222727

Purpose

sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Tags

sfGFP

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHIV-Luciferase

Plasmid

#21375

Purpose

self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase

Depositor

Bryan Welm

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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TFORF2061

Plasmid

#141900

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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