We narrowed to 13,735 results for: 109

Showing: 9861 - 9880 of 13735 results

Results per page:

Sort results by:

pX459V2.0-HypaCas9-mRuby2

Plasmid

#183872

Purpose

pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Type

Empty backbone

Use

CRISPR

Tags

HypaCas9-P2A-mRuby2-T2A-Puro

Expression

Mammalian

Promoter

Cbh

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-CAG-ECC-Biosensor

Plasmid

#140832

Purpose

Encoding the CAG promoter based eccDNA biosensor

Depositor

Yonglun Luo

Article

Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

Insert

ECC-Biosensor

Use

Piggybac (pb) transposon

Expression

Mammalian

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBHM2406

Plasmid

#173442

Purpose

pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

Depositor

Hiten Madhani

Article

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

mNeonGreen-CBP-2XFLAG-HYGB

Tags

CBP-2xFLAG

Expression

Bacterial and Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1.ARRDC1

Plasmid

#38320

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

ARRDC1 (ARRDC1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Wild-type AdPol vector

Plasmid

#131685

Purpose

A lentiviral vector that constitutively expresses the wild-type adenovirus DNA polymerase

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Insert

Adenovirus DNA polymerase

Use

Lentiviral

Tags

HA

Available Since

June 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Accessory plasmid 1

Plasmid

#128569

Purpose

for mixing with SPECS library to produce lentivirus

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Insert

ECFP

Use

Lentiviral

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-FNLSNG-P2A-Puro

Plasmid

#136900

Purpose

Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSNG

Use

Lentiviral

Tags

FLAG-NLS and NLS

Mutation

L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

Promoter

EF1s

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
KP501: pMVP (L5-L4) eYFP-P2A

Plasmid

#121714

Purpose

pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP-P2A (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM320 - sgRNA mosTI unc-119 array insertion

Plasmid

#159824

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 array insertion

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-MSP1D1delltaH4

Plasmid

#71713

Purpose

Membrane scaffold protein (MSP), helix 4 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+ gfp-zASC

Plasmid

#98703

Purpose

GFP tagged zebrafish ASC in the pCS2+ vector for in vitro transcription

Depositor

Anna Huttenlocher

Article

Vincent et al Cell Microbiol. 2016 Apr;18(4):591-604.

Insert

Apoptosis associated speck like protein containing CARD (pycard Zebrafish)

Use

Zebrafish/xenopus expression

Tags

GFP

Expression

Mammalian

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLYS5-MCU-Flag

Plasmid

#50054

Purpose

Expression of human MCU in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

MCU-Flag (MCU Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pmGFP-Sec16S

Plasmid

#15775

Depositor

Benjamin Glick

Article

Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

Insert

Sec16S (SEC16B Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N/A

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-mNectarine

Plasmid

#21717

Purpose

Bacterial expression of pH-sensitive monomeric mNectarine red fluorescent protein

Depositor

Robert Campbell

Article

Johnson et al J Biol Chem. 2009 Jul 31;284(31):20499-5

Insert

mNectarine fluorescent proteins

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pYFJ16-LplA(W37V) (for E. Coli expression)

Plasmid

#34838

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA W37V (lplA E. Coli)

Tags

His Tag

Expression

Bacterial

Mutation

Mutated Trp37 to Val

Promoter

T5 promoter

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
linker-mCitrine-PIF6-NAT

Plasmid

#51576

Purpose

Tag protein of interest with PIF6 in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PIF6 (PIL2 Mustard Weed)

Tags

linker and mCitrine

Expression

Yeast

Mutation

1-100aa of PIF6

Promoter

none

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LC201: pMVP/PB/Puro-DEST

Plasmid

#121874

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP‐RNASEH2C

Plasmid

#108698

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2C in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2C (RNASEH2C Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-His-hCE

Plasmid

#112710

Purpose

Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNGTT (RNGTT Human)

Tags

6xHis and Stop codons placed after RNGTT sequence…

Expression

Bacterial

Mutation

R594H

Promoter

T7

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPP5052

Plasmid

#128729

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopAM1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge