We narrowed to 17,309 results for: ESC
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Plasmid#178971PurposeExpresses TagRFP658 and GFP in mammalian cellsDepositorInsertTagRFP658-P2A-GFP
UseAAVExpressionMammalianPromoterCAGAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL
Plasmid#169533PurposeTier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).DepositorInsertPCMV-driven ER-localized monomeric red fluorescent protein mRuby2
ExpressionMammalianPromoterPhCMVAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53_SNCB+_attB53
Plasmid#183614PurposeRMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.DepositorInsertsLaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP
UseRmceTagsMyc and human CD8a signal sequenceExpressionMammalianAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H21
Plasmid#170335PurposeCFP(ST)_EPAC full length_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorAvailable SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TPC1(ΔN69)-mCherry
Plasmid#135184PurposeTPC1 with truncated N-terminus, tagged with mCherry on C terminus.DepositorInsertTPC1 (Tpcn1 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationDeleted amino acids 1-69Available SinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDM358_DPAKa(GBD)-YFP
Plasmid#128700Purposeexpression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cellsDepositorInsertGBD domain (aa 731-890) of DPAKa
UseDictyostelium discoideum expression vectorTagsYFPMutationnoneAvailable SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]
Plasmid#115893PurposeAAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorHas ServiceAAV8InsertArchon1-KGC-EGFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianPromoterSynAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
aav-CAG-FLEX-tdNfsB(F124W)-mCherry (eNTR)
Plasmid#113762PurposeAAV-mediated conditional expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoterDepositorInserttdNfsB(F124W)-mCherry
UseAAV and Cre/LoxTagsmCherryExpressionMammalianMutationF124WPromoterCAGAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gucy1b2-IRES-tauGFP-ACNF TV
Plasmid#105211Purposetargeting vector to generate a Gucy1b2-IRES-tauGFP mouse strain.DepositorInsertGucy1b2-IRES-tauGFP-ACNF (Gucy1b2 Mouse)
UseMouse TargetingAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Nup35-mut
Plasmid#87341PurposeTo express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP35 (NUP35 Human)
TagsEGFPExpressionMammalianMutationshRNA resistant changesPromoterCMVAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4B (205-224) (pGEX2T(TEV))
Plasmid#80634PurposeResidues 205-224 of CHMP4B, Bacterial Expression Vector; TEV-cleavabel GST tag; Sundquist Lab Internal ID: WISP06-201DepositorAvailable SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-C4
Plasmid#73427PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.DepositorInsertRepressor C4 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA8WTmneo
Plasmid#69609PurposeExpresses a neomycin tagged wildtype L1PA8DepositorInsertL1PA8
Tagsmneo retrotransposition cassetteExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
tdEos-FilaminA-C-14
Plasmid#57615PurposeLocalization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorAvailable SinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
psbA2-Ptrc-T7-g10-PHLS (e)
Plasmid#52310PurposeReplaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyExpressionBacterialPromoterPtrc-T7Available SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1032
Plasmid#52997PurposepLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
ompC
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −56 to +141 bp relative to start codon a…PromoterpCon, pLlacO-1, and pLtetO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGST1-GGA1-VHS
Plasmid#44418DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Txk-SH2
Plasmid#46526DepositorInsertTxk (Txk Mouse)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 138-258PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Slap2-SH2
Plasmid#46501DepositorInsertSlap2 (SLA2 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 84-197PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX ShcC-SH2
Plasmid#46487DepositorInsertShcC (SHC3 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 485-594PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only