We narrowed to 22,940 results for: REP

Showing: 9961 - 9980 of 22940 results

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pcDNA-LoxP-mKate-LoxP-EGFP

Plasmid

#167940

Purpose

CMV promoter-LoxP-mKate-LoxP-EGFP

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

LoxP-mKate-LoxP-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLX-B2 (GB3374)

Plasmid

#160636

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)

Depositor

Juan Antonio García, Fabio Pasin

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAPM-D4- miR30-L1221

Plasmid

#115846

Purpose

Control shRNA KD vector

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

control shRNA

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTP284

Plasmid

#113312

Purpose

human Nbs1 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Myler et al Mol Cell. 2017 Sep 7;67(5):891-898.e4. d

Insert

Nbs1 (NBN Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTre-Tight-luciferase-GFP (467)

Plasmid

#65491

Purpose

negative control for expression assays

Depositor

Roderic Guigo, Rory Johnson

Insert

EGFP

Use

Luciferase

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3

Plasmid

#67276

Purpose

RMCE donor plasmid for FlpO. the marker gene is green fluorescent (mWasabi) with PA

Depositor

Jannik Elverløv-Jakobsen

Article

Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

Inserts

GFP (mWasabi)
hygro

Tags

F3 and Frt

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Gata1

Plasmid

#41085

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Gata1 (Gata1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO + minimal CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmAGO1

Plasmid

#22768

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

Argonaut-1, variant A (AGO1 Fly)

Use

Drosophila expression

Tags

EGFP

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pSUC01

Plasmid

#177204

Purpose

chromogenic reporter for gene knockout in Streptomyces

Depositor

Wenjun Guan

Article

Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

Insert

SaindC

Use

Synthetic Biology

Tags

NONE

Expression

Bacterial

Mutation

NONE

Promoter

TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pVCERC-5 Pvan-dcas9 (Sth3)

Plasmid

#133319

Purpose

expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus thermophilus #3)

Use

Unspecified

Promoter

vanillate

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA6-mRuby3

Plasmid

#184892

Purpose

Stable expression of ANXA6-mRuby3 (Annexin A6-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA6 (ANXA6 Human)

Tags

mRuby3

Expression

Mammalian

Mutation

R587K

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

Plasmid

#177920

Purpose

Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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A2_uSFFV

Plasmid

#153415

Purpose

Expresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

A*02:01-P2A-human beta2 microglobulin (B2M Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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RP4-6

Plasmid

#80385

Purpose

Helper plasmid for mobilisation derived from RP4. Spectinomycin/Streptomycin resistant

Depositor

Michael Hynes

Article

Quandt et al Plasmid. 2004 Jul;52(1):1-12.

Insert

Spectinomycin/Streptomycin

Use

Helper bhr plasmid for mobilizing other plasmids …

Tags

none

Promoter

None

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only
Neurod1-Atoh7HA3

Plasmid

#44438

Depositor

William Klein

Article

Mao et al Development. 2013 Feb;140(3):541-51. doi

Insert

Atoh7-3xHA knock-in (Atoh7 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Neurod1 left arm, Neurod1 right arm, and loxC2-Re…

Expression

Mammalian

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-(SUMO)3

Plasmid

#127093

Purpose

Bacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)

Tags

EGFP

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

Plasmid

#84604

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Article

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Promoter

8xGliBS-delta-crystallin minimal promoter

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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MSP712

Plasmid

#65768

Purpose

Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Bacterial

Mutation

D10A and H840A mutations in Cas9

Promoter

T7 (x2)

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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Sth1Cas9n cytosine base editor

Plasmid

#194209

Purpose

Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

Sth1Cas9-D9A nickase

Use

CRISPR

Expression

Bacterial

Mutation

Changed Aspartate 9 to Alanine.

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC468 - pAAV EF1a DIO hChR2(H134R)-iRFP

Plasmid

#47633

Purpose

An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

hChR2 (H134R)

Use

AAV and Cre/Lox

Tags

iRFP

Expression

Mammalian

Mutation

H134R

Promoter

EF1a

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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