161,273 results

1. pCS Omomyc-Mer

   Plasmid

   #113170

   Purpose

   Expression of Omomer (tamoxifen inducible Omomyc) in mammalian cells

   Depositor

   Sergio Nasi

   Insert

   Omomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E57T, E64I, R70Q, R71N

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pAAV.GFAP.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209661

   Purpose

   Expression of iATPSnFR2, low affinity

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95A.A119L

   Promoter

   GFAP

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-GFAP-eLACCO1.1

   Plasmid

   #168790

   Purpose

   Astrocyte expression of fluorescent sensor for extracellular lactate

   Depositor

   Marie-Eve Paquet

   Article

   Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

   Insert

   eLacco1.1
   

   Use

   AAV

   Tags

   ER export and GPI

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_MAX_WT_V5

   Plasmid

   #82944

   Purpose

   Gateway Donor vector containing MAX, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Matthew Meyerson, David Root, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   MAX (MAX Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDest17_MAX

   Plasmid

   #234044

   Purpose

   Bacterial expression of N-terminally 6His-tagged MAX; includes TEV cleavage site

   Depositor

   Tanja Mittag

   Article

   Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

   Insert

   MAX (MAX Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAdDeltaF6

   Plasmid

   #112867

   Purpose

   AAV helper plasmid

   Depositor

   James M. Wilson

   Insert

   E4, E2a and VA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCas9-Blast

   Plasmid

   #52962

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Lentiviral Prep

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMDLg/pRRE

   Plasmid

   #12251

   Purpose

   3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Article

   Dull et al J Virol. 1998 Nov . 72(11):8463-71.

   Insert

   HIV-1 GAG/POL
   

   Use

   Lentiviral; Packaging

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+) eGFP

   Plasmid

   #129020

   Purpose

   Expresses eGFP

   Depositor

   Jeremy Wilusz

   Article

   Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-tevopreq1-GG-acceptor

    Plasmid

    #174038

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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