4,177 results

1. 8xGTIIC-luciferase

   Plasmid

   #34615

   Purpose

   YAP/TAZ-responsive synthetic promoter driving luciferase expression

   Depositor

   Stefano Piccolo

   Article

   Dupont et al Nature. 2011 Jun 8;474(7350):179-83. doi

   Insert

   synthetic TEAD luciferase reporter
   

   Use

   Luciferase

   Available Since

   March 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUW-M2rtTA

   Plasmid

   #20342

   Purpose

   Lentiviral plasmid expressing the reverse tetracycline transactivator

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

   Insert

   M2rtTA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. scramble shRNA

   Plasmid

   #1864

   Purpose

   3rd gen lentiviral negative control vector containing scrambled shRNA

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   scramble
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1a-IRES-Puro

   Plasmid

   #85132

   Purpose

   lentiviral backbone

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCXLE-hUL

   Plasmid

   #27080

   Purpose

   Integration-free (episomal) expression of human L-MYC and LIN28

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   L-MYC (LIN28A, MYCL Human)
   

   Expression

   Mammalian

   Available Since

   March 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMDLg/pRRE

   Plasmid

   #12251

   Purpose

   3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Article

   Dull et al J Virol. 1998 Nov . 72(11):8463-71.

   Insert

   HIV-1 GAG/POL
   

   Use

   Lentiviral; Packaging

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET29b-10xHis-Super TEV

   Plasmid

   #193833

   Purpose

   Expresses high-efficiency TEV protease in bacterial cells

   Depositor

   Florence Pojer, EPFL Protein Production and Structure Core Facility (PTPSP)

   Article

   Lau et al protocols.io, doi: 10.17504/protocols.io

   Insert

   TEV protease
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR v2-Blast

   Plasmid

   #83480

   Purpose

   Mammalian expression of Cas9 and sgRNA scaffold

   Depositor

   Mohan Babu

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   EF-1α

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. DHB-mVenus

   Plasmid

   #136461

   Purpose

   Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

   Depositor

   Sabrina Spencer, Tobias Meyer

   Article

   Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

   Insert

   DNA Helicase B (HELB Human)
   

   Use

   Lentiviral

   Tags

   mVenus

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA

    Plasmid

    #61591

    Purpose

    A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Available Since

    Feb. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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