1,878 results

1. T2A 3xNLS GFP

   Plasmid

   #227721

   Purpose

   Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Haosheng Sun, Oliver Hobert

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ350 - MCS(ttTi5605, II)

   Plasmid

   #34866

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   ttTi5605 targeting
   

   Use

   Worm targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF11-wmiRFP713-T2A-HO1

   Plasmid

   #197246

   Purpose

   Expresses the protein of wmiRFP713-T2A-HO1 in neurons of C. elegans

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   wmiRFP713-T2A-HO1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDD286 (TagRFP^SEC^3xMyc)

   Plasmid

   #70684

   Purpose

   TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.

   Depositor

   Bob Goldstein

   Insert

   TagRFP-T-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xMyc and C. elegans codon-optimized TagRFP-T

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET_MBP-RGG-GFP-RGG

   Plasmid

   #124949

   Purpose

   Expression of MBP-RGG-GFP-RGG

   Depositor

   Daniel Hammer, Matthew Good, Benjamin Schuster

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   MBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   EGFP, His, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAD48-daf-2 RNAi

   Plasmid

   #34834

   Depositor

   Cynthia Kenyon

   Article

   Dillin et al Science. 2002 Oct 25;298(5594):830-4.

   Insert

   daf-2 (daf-2 Nematode)
   

   Use

   RNAi

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 11, 2012

   Availability

   Academic Institutions and Nonprofits only

7. JH01

   Plasmid

   #73510

   Purpose

   GFPo_PH pCFJ150 - contains C. elegans sequence optimized GFP

   Depositor

   Bob Goldstein

   Article

   Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

   Insert

   pmex-5::GFPo::PH::tbb-2
   

   Use

   Tags

   GFP

   Expression

   Mutation

   Promoter

   mex-5

   Available Since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA_RGG-GFP-RGG

   Plasmid

   #124930

   Purpose

   Expression of RGG-GFP-RGG

   Depositor

   Daniel Hammer, Matthew Good, Benjamin Schuster

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSF11-wmCardinal

   Plasmid

   #197251

   Purpose

   Expresses the protein of wmCardinal in neurons of C. elegans

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   wmCardinal
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBN42

    Plasmid

    #86713

    Purpose

    C. elegans injection marker

    Depositor

    Peter Askjaer

    Article

    Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

    Insert

    Pmyo-3::GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEY14

    Plasmid

    #191059

    Purpose

    acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-5(orf) (acr-5 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pdestDD04Neo

    Plasmid

    #26386

    Depositor

    Denis Dupuy

    Article

    Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Worm

    Mutation

    Promoter

    Available Since

    Oct. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCFJ356 - MCS(cxTi10816, IV)

    Plasmid

    #34871

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    cxTi10816 targeting
    

    Use

    Worm targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid

    #154840

    Purpose

    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    HYGR∆ encodes aa 1-226

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]

    Plasmid

    #159817

    Purpose

    Positive control for germline GFP expression from simple array in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. tph-1(short)::rpl-22-HA

    Plasmid

    #122203

    Purpose

    C. elegans tagged ribosome expression plasmid

    Depositor

    Steven Flavell

    Article

    Rhoades et al Cell. 2019 Jan 10;176(1-2):85-97.e14. do

    Insert

    tph-1(short)::rpl-22-HA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

    Plasmid

    #159808

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET_RGG-RGG

    Plasmid

    #124941

    Purpose

    Expresion of RGG-RGG

    Depositor

    Daniel Hammer, Matthew Good, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCFJ212 - pDESTR4-R3(cxTi10816, IV)

    Plasmid

    #34864

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    cxTi10816 targeting
    

    Use

    Worm targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159857

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

    Plasmid

    #193048

    Purpose

    Heatshock expressed Cas9

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    hsp16.41p

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSEM231 - [Pmlc-1 | gfp | cbr-tbb-2 UTR]

    Plasmid

    #159897

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | gfp | cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCFJ2369 - intron GG1 - 250bp

    Plasmid

    #159877

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG1 - 250bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJA58

    Plasmid

    #59933

    Purpose

    gRNA for cleavage at dpy-10(cn64) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    dpy-10(cn64) gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)

    Plasmid

    #193049

    Purpose

    Encodes G-418 resistance

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    prsp-27::NeoR::unc-54 3’ UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    prsp-27

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJW1659

    Plasmid

    #154314

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::AID*::3xFLAG for NT slot
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCFJ421 - Pmyo-2::GFP::H2B (pharynx)

    Plasmid

    #34876

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    Pmyo-2 GFP H2B tbb-2 UTR
    

    Use

    Worm targeting

    Tags

    Expression

    Mutation

    S65C

    Promoter

    myo-2

    Available Since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. coel::GFP

    Plasmid

    #8937

    Depositor

    Piali Sengupta

    Article

    Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

    Insert

    unc-122 promoter (unc-122 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNM3823

    Plasmid

    #154123

    Purpose

    mec-4 promoter driving tet OFF tetR-L-QFAD in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p::tetR-L-QFAD::act-4 '3
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNM3755

    Plasmid

    #154116

    Purpose

    tetO 7X ∆pes-10 promoter driving GFP-C1 & an act-4 3' UTR in a RMCE vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    tetO 7X::∆pes-10::GFP-C1::act-4 3'
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge