1,927 results
-
Plasmid#110166PurposeminiMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)DepositorInsertERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
UseMinimos transposonTagsmCherry and mCloverExpressionWormMutationPhospho-acceptor sites mutated: S43A, T55A, T62A.…Promoterlin-31 promoter/enhancerAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOD2044-intDEG
Plasmid#89366PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)PromoterPelt-2Available SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNMSB104
Plasmid#199314PurposeMosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605DepositorInsertflp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR
ExpressionWormMutationmTagBFP2 from pJJR81, C. elegans codon optimized …Promoterflp-18Available SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEM253 - sgRNA mosTI hygroR
Plasmid#159827PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertsgRNA mosTI hygroR
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCZGY3196
Plasmid#172696PurposePrgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)DepositorInsertPrgef-1
TagsGFP 1-10ExpressionWormAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM270 - MCS mosTI hygroR target
Plasmid#159826PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertMCS mosTI hygroR target
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD286 (TagRFP^SEC^3xMyc)
Plasmid#70684PurposeTagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.DepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xMyc and C. elegans codon-optimized TagRFP-TExpressionWormAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Prab-3::NLS::GFP
Plasmid#68120PurposeNuclear Localized GFP for pan-neuronal expression in C. ElegansDepositorInsertGFP
ExpressionWormPromoterrab-3Available SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xHA-miniTurbo_pAS32
Plasmid#118222Purposeexpresses 3xHA-tagged miniTurbo in C. elegans intestineDepositorInsertminiTurbo (BirA mutant)
Tags3x HA tagExpressionWormMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterges-1pAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-sCAG-GluClβ.CreON
Plasmid#196996PurposeCre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.DepositorInsertGluClβ -YFP
UseAAV and Cre/LoxTagsYFPPromotershort CAGAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RGG_withTEVsite
Plasmid#124940PurposeExpression of RGG-RGGDepositorInsertRGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsHisExpressionBacterialMutationwith TEV site between RGG domainsAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Prab-3::NLS::GCaMP6s
Plasmid#68119PurposeNuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. ElegansDepositorInsertrab-3::NLS::GCaMP6s
ExpressionWormPromoterrab-3Available SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hsf-1-sgRNA-B
Plasmid#177787PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)DepositorInserthsf-1 (hsf-1 Nematode)
ExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]
Plasmid#159807PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
Plasmid#158967PurposeUbiquitous expression of mtON in C. elegansDepositorInserteft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
TagsGFPExpressionWormPromotereft-3Available SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHJL2
Plasmid#199377PurposeEncodes dual cistronic cytosolic GCaMP6f and mScarlet-I optimized for C. elegans expressionDepositorInsertGCaMP6f::SL2::mScarlet-I
ExpressionBacterialMutationno mutationsAvailable SinceApril 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSF11-wmiRFP670-2-T2A-HO1
Plasmid#197250PurposeExpresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegansDepositorInsertwmiRFP670-2-T2A-HO1
ExpressionWormAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMLS262
Plasmid#73718PurposeC. elegans Pan-neuronal 2xNLS-FLP-D5 expression vectorDepositorInsertPsnt-1::2xNLS-FLP-D5
ExpressionWormMutationG5DPromoterPsnt-1Available SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCG150
Plasmid#17247DepositorInsertunc-119 rescue fragment in pDESTR4R3
UseGateway CloningAvailable SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-sCAG-GluClα.CreON
Plasmid#196995PurposeCre recombinase dependent expression of GluClv2.0 alpha subunit. GluClα contains a Cerulean tag. When co-expressed with GluClv2.0 beta subunit, agonist (Ivermectin) induces neuronal silencing.DepositorInsertGluClα -Cerulean
UseAAV and Cre/LoxTagsCeruleanPromotershort CAGAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1955
Plasmid#84830PurposeMinimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized tdTomato
ExpressionBacterial and WormPromoterPeft-3Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOD2045-intControl
Plasmid#100005PurposeMosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).DepositorInsertsUseTargeting vector for mos1 transposon mediated sin…Available SinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSA102
Plasmid#59788Purposedrives mCherry in C. elegans neuronsDepositorInsertsrab-3 promoter
mCherry (worm)
unc-54 3' UTR
Available SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH3557
Plasmid#191444PurposePttr-39-Chrimson::wCherry unc-54 3' UTR C.elegans D motor neurou expression of Chrimson RFPDepositorInsertChrimson
TagswCherryExpressionWormPromoterPttr-39Available SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS260
Plasmid#73685PurposePunc-17::2xNLS-FLP-D5 Expression vectorDepositorInsertPunc-17::2xNLS-FLP-D5::let-858 3'-UTR
UseFlp/frtExpressionWormMutationG5DPromoterPunc-17Available SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLS360
Plasmid#73687PurposePunc-47::2xNLS-FLP-D5 Expression vectorDepositorInsertPunc-47::2xNLS-FLP-D5::let-858 3'-UTR
UseFlp/frtExpressionWormPromoterPunc-47Available SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pflp-18::NGL-1::spGFP11
Plasmid#65828PurposeFor trans-synaptic labelingDepositorAvailable SinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEY11
Plasmid#191017PurposeFluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)DepositorInsertUltrapanneuronal
ExpressionWormMutationNoneAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH4687
Plasmid#200783PurposePnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorinDepositorAvailable SinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJF5
Plasmid#139031PurposeMosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette systemDepositorInsertgfp (no STOP codon, PATC introns)
ExpressionWormAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only