We narrowed to 303 results for: bmf

1. xlox dNGFR-TERT

   Plasmid

   #69805

   Purpose

   MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a tNGFR marker for tracking

   Depositor

   David Ott

   Article

   Andersen et al AIDS Res Hum Retroviruses. 2007 Mar;23(3

   Insert

   hTERT reverse transcriptase (TERT Human)
   

   Use

   Retroviral

   Tags

   none

   Expression

   Mutation

   Promoter

   MLV promoter

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NSK026 CMV-TO-p53R248W (FLP-IN)

   Plasmid

   #191168

   Purpose

   Expression of human p53 mutant R248W in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   p53(R248W) (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R248W

   Promoter

   CMV-TO

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-pro-siRNA-V2-TP53

   Plasmid

   #111089

   Purpose

   pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.

   Depositor

   Linfeng HUANG

   Article

   Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

   Insert

   TP53 (TP53 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT5T_hp53_ST

   Plasmid

   #34922

   Depositor

   Thanos Halazonetis

   Article

   Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

   Insert

   p53 DNA-binding (Res 1-393) (TP53 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

   Promoter

   T7

   Available since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TFORF1345

   Plasmid

   #142788

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RPA1 (RPA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-HA-p53 2KR

   Plasmid

   #78799

   Purpose

   To overexpress p53 2KR in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K291R, K292R

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast

   Plasmid

   #166413

   Purpose

   express hPOT1 ΔOB in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

   Insert

   Protection Of Telomeres 1 (POT1 Human)
   

   Use

   Lentiviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   ΔOB deleted 125 a.a

   Promoter

   ef1-a

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28-p53 (1-73) (72P)

   Plasmid

   #62083

   Purpose

   Expression of human p53 (1-73) (72P) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   HIs

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; 72P

   Promoter

   T7

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

9. MSCVpic2neo-hTERT-FF2

   Plasmid

   #164910

   Purpose

   Expresses hTERT cDNA and FF2 shRNA in mammalian cells

   Depositor

   Ji Luo

   Article

   Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

   Inserts

   human telomerase reverse transcriptase (TERT Human)
   Neuropeptide FF receptor 2 (NPFFR2 Human)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-p53 (1-73) (72R)

    Plasmid

    #62082

    Purpose

    Expression of human p53 (1-73) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72R

    Promoter

    T7

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pX61

    Plasmid

    #46852

    Purpose

    TOP Hupki-puro2A-Hupki-wt plasmid

    Depositor

    Monica Hollstein

    Article

    Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

    Insert

    attB-Puro-2A and Hupki p53 wild type (TP53 Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330-TP53

    Plasmid

    #227318

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of TP53 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of TP53 (TP53 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL4.0-TERT_-100_-79

    Plasmid

    #193785

    Purpose

    luciferase reporter for duplicated TERT promoter

    Depositor

    Joseph Costello

    Article

    Barger et al Nat Commun. 2022 Sep 16;13(1):5430. doi:

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TERT

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. H6GB1tevP53(304-393)

    Plasmid

    #200313

    Purpose

    bacterial expression of p53 TET-CTD

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53(304-393) (TP53 Human)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. H6GB1tevP53(363-393)

    Plasmid

    #200314

    Purpose

    bacterial expression of P53 CTD

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53(363-393) (TP53 Human)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLPC-N-Myc-Flag-BirA-hPOT1-DeltaOB

    Plasmid

    #166410

    Purpose

    express BirA-hPOT1 ΔOB in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    POT1 ΔOB (POT1 Human)
    

    Use

    Tags

    MYC-flag-BirA

    Expression

    Mammalian

    Mutation

    ΔOB

    Promoter

    cmv

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-p53 (1-73) (K24N)

    Plasmid

    #62079

    Purpose

    Expression of human p53 (1-73) (K24N) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; K24N

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pX41

    Plasmid

    #46851

    Purpose

    TOP Hupki-wt plasmid without selection marker

    Depositor

    Monica Hollstein

    Article

    Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

    Insert

    attB and Hupki p53 wild type (TP53 Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti6/V5-p53_wt p53

    Plasmid

    #22945

    Purpose

    3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tag

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIRES2-mCherry-p53 deltaN

    Plasmid

    #49243

    Purpose

    expresses human p53 deltaN and mCherry in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53 deltaN (TP53 Human)
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    deltaN ( lacks 39 residues at the N-terminus)

    Promoter

    CMV

    Available since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLentiCRISPR_zeo_sgTP53

    Plasmid

    #183180

    Purpose

    pLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgTP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGL4.0-TERT WT

    Plasmid

    #84924

    Purpose

    luciferase reporter for TERT promoter

    Depositor

    Joseph Costello

    Article

    Bell et al Science. 2015 May 29;348(6238):1036-9. d

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    TERT

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FLAG-LoxP-GFP-LoxP-SNAP-TERT

    Plasmid

    #71391

    Purpose

    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Use

    Hr donor

    Tags

    FLAG-SNAP

    Expression

    Mutation

    Promoter

    Endogenous TERT promotor

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EF1a_Puro_Telo_v2

    Plasmid

    #195139

    Purpose

    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Telomere (RTEL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EF1a_Puro_Telo_v1

    Plasmid

    #195138

    Purpose

    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Telomere (RTEL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGL4.0-TERT G228A

    Plasmid

    #84926

    Purpose

    luciferase reporter for mutant TERT promoter

    Depositor

    Joseph Costello

    Article

    Bell et al Science. 2015 May 29;348(6238):1036-9. d

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    G228A

    Promoter

    TERT

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL4.0-TERT G250A

    Plasmid

    #84925

    Purpose

    luciferase reporter for mutant TERT promoter

    Depositor

    Joseph Costello

    Article

    Bell et al Science. 2015 May 29;348(6238):1036-9. d

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    G250A

    Promoter

    TERT

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

28. p11d-tRPA (MSW#111)

    Plasmid

    #208066

    Purpose

    expression of human Replication Protein A in E. coli

    Depositor

    Marc Wold

    Article

    Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

    Insert

    RPA1, RPA2, RPA3 (RPA1, RPA3, RPA2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    RPA32 has a point mutation Thr88(ACC) -> Ile(A…

    Promoter

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRRLNeo-pEF1a-p53ash-mCerulean

    Plasmid

    #69579

    Purpose

    Expresses p53 tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    7 silent mutations in p53 DNA to prevent targetin…

    Promoter

    EF1alpha

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC

    Plasmid

    #69583

    Purpose

    Expresses p53 tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    7 silent mutations in p53 DNA to prevent targetin…

    Promoter

    EF1alpha

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pIRES2-EGFP-p53 tethered

    Plasmid

    #49245

    Purpose

    expresses two human WT p53s tethered together and also expresses EGFP in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53-p53 (TP53 Human)
    

    Use

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HR-TERT-SV40-GFP

    Plasmid

    #71397

    Purpose

    Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Use

    Tags

    SV-40 GFP insertion in TERT promotor

    Expression

    Mutation

    Promoter

    Endogenous TERT promotor

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. H6SUMO-IntC-P53(304-393)

    Plasmid

    #200316

    Purpose

    bacterial expression of P53 TET-CTD for segmental labeling

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (304-393) (TP53 Human)
    

    Use

    Tags

    His6-SUMO and designed intein C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. H6GB1tevP53(1-303)IntN

    Plasmid

    #200312

    Purpose

    Bacterial expression of P53 NTAD-DBD for segmental labeling

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His6GB1tev and designed intein N

    Expression

    Bacterial

    Mutation

    M133L, V203A, N239Y, N268D

    Promoter

    T7

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28-p53 (1-90) (72P)

    Plasmid

    #62080

    Purpose

    Expression of human p53 (1-90) (72P) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD/PP residues 1-90; 72P

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pT5T_hp53_ST_CR2

    Plasmid

    #34923

    Depositor

    Thanos Halazonetis

    Article

    Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

    Insert

    p53 DNA-binding (Res 94-358, deletion 293-321) (TP53 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

    Promoter

    T7

    Available since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pX62

    Plasmid

    #46854

    Purpose

    TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCE

    Depositor

    Monica Hollstein

    Article

    Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

    Insert

    attB-Puro-2A and Hupki p53 G245S (TP53 Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G245S

    Promoter

    Available since

    Aug. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28A-p53 (1-73) (P to A)

    Plasmid

    #62069

    Purpose

    Expression of human p53 (1-73) (all P to A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; all Prolines changed …

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

39. Luc-Hdm4-3'UTR-F2d5

    Plasmid

    #64018

    Purpose

    Luciferase reporter of human Hdm4 3'UTR F2 fragment mutant #5

    Depositor

    Lin He

    Article

    Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi:

    Insert

    HDMX (MDM4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28A-p53 (1-73) (P27A)

    Plasmid

    #62068

    Purpose

    Expression of human p53 (1-73) (P27A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; P27A

    Promoter

    T7

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only