Skip to main content
Addgene
Showing: 161 - 200 of 847 results

  1. AAV-DIO-TC66T-2A-EGFP-2A-oG

    Plasmid
    #178429
    Purpose
    Expresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    Edward Callaway
    Inserts
    TVA950 Glu66→Thr
    Optimized G
    EGFP
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178429-plasmid-map-sequence-id-354737

    Enlarge

  3. groSL-GFP

    Plasmid
    #109389
    Purpose
    GFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    pgroSL-GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109389-plasmid-map-sequence-id-210771

    Enlarge

  4. ibpAB-GFP

    Plasmid
    #109390
    Purpose
    GFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    pibpAB-GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109390-plasmid-map-sequence-id-210782

    Enlarge

  5. htpG1-GFP

    Plasmid
    #109387
    Purpose
    GFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    phtpG1-GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109387-plasmid-map-sequence-id-210728

    Enlarge

  6. htpG2-GFP

    Plasmid
    #109388
    Purpose
    GFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    phtpG2-GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109388-plasmid-map-sequence-id-210731

    Enlarge

  7. pETcLIC-GFP

    Plasmid
    #183514
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    June 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    183514-plasmid-map-sequence-id-362488

    Enlarge

  8. pYUBcLIC-GFP

    Plasmid
    #183509
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    183509-plasmid-map-sequence-id-362497

    Enlarge

  14. pAd5-B6/7 ∆E3-GFP

    Plasmid
    #179202
    Purpose
    A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6∆E3-GFP and pAd5-B7.
    Depositor
    Fred Bunz
    Article
    Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do
    Insert
    Modified block 6/7, with deletion in E3 and insertion of GFP cassette
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    179202-plasmid-map-sequence-id-351756

    Enlarge

  18. mT2A-sfGFP-linker-iCre-ERT2-FNF

    Plasmid
    #182341
    Purpose
    Recombineering cassette plasmid for T2A-sfGFP-linker-iCre-ERT2-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-sfGFP-linker-iCre-ERT2-FNF
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    182341-plasmid-map-sequence-id-359742

    Enlarge

  27. pLXB3o1 HygR-MAR10-gemiGFP-MAR10 (GB4684)

    Plasmid
    #225439
    Purpose
    Module for stable transformation of Geminino 1.0-eGFP flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.
    Depositor
    Diego Orzaez
    Insert
    HygR-MAR10-gemiGFP-MAR10
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225439-plasmid-map-sequence-id-447723

    Enlarge

  32. INT_Cargo_T7_gfp

    Plasmid
    #212136
    Purpose
    Cargo plasmid for integrating PT7 expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Article
    Fung et al Biomaterials. 2022 Aug;287:121661. doi:
    Insert
    green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212136-plasmid-map-sequence-id-420767

    Enlarge

  34. 5UAS zfSynaptophysin:GFP-5UAS DsRedExpress

    Plasmid
    #74317
    Purpose
    A fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafish
    Depositor
    Stephen Smith, Martin Meyer
    Article
    Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.
    Inserts
    synaptophysin
    DsRedExpress
    Use
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    5UAS
    Available since
    May 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    74317-plasmid-map-sequence-id-314170

    Enlarge

  35. LV TRE-GFP-TMD-DLL1

    Plasmid
    #194936
    Purpose
    LV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.
    Depositor
    John Ngo
    Article
    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Insert
    eGFP-(PDGFR)TMD-rDLL1 ICD (WT)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194936-plasmid-map-sequence-id-386565

    Enlarge

  38. pCALNL-eGFP-Esp3I

    Plasmid
    #81226
    Purpose
    Cloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sites
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    Esp31-Neo-Esp31 deletion cassette upstream of EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCBa
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    81226-plasmid-map-sequence-id-168098

    Enlarge

Showing: 161 - 200 of 847 results