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We narrowed to 5,443 results for: 129

Showing: 201 - 250 of 5443 results

  1. pN1-COX8-mScarletI

    Plasmid
    #129341
    Purpose
    Mitochondria matrix localization
    Depositor
    Yasushi Okada
    Insert
    mScarlet(i) (Addgene 85044)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    129341-plasmid-map-sequence-id-260104

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  2. pAH25-SceI

    Plasmid
    #129389
    Purpose
    Derived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Chloramphenicol acetyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N/A
    Promoter
    CAT promoter
    Available since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129389-plasmid-map-sequence-id-258595

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  4. KIF3A406

    Plasmid
    #129768
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    129768-plasmid-map-sequence-id-271499

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  5. pGEX-2T-Cdc42-Q61L

    Plasmid
    #12971
    Depositor
    Gary Bokoch
    Insert
    Cdc42 constitutively active (CDC42 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Q61L Constitutively active
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  7. KRigor980

    Plasmid
    #129763
    Purpose
    Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    R210A
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129763-plasmid-map-sequence-id-263579

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  12. KIF3A560GFP

    Plasmid
    #129769
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129769-plasmid-map-sequence-id-263530

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  19. pGEX-2T-Rac1-T17N

    Plasmid
    #12978
    Depositor
    Gary Bokoch
    Insert
    Rac1 DN (RAC1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    T17N Dominant negative
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  20. pGEX-5X-1 U1-C-FL

    Plasmid
    #129077
    Purpose
    generation of GST-tagged U1-C protein using bacteria
    Depositor
    Takeshi Tsubata
    Insert
    U1-C (SNRPC Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    129077-plasmid-map-sequence-id-252125

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  21. pN1-OPA1(123Nt)-mScarletI

    Plasmid
    #129343
    Purpose
    Mitochondrial intermembrane space localization
    Depositor
    Yasushi Okada
    Insert
    mScarlet(i) (Addgene 85044)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    129343-plasmid-map-sequence-id-260106

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  22. pGEX-2T-Cdc42-T17N

    Plasmid
    #12970
    Depositor
    Gary Bokoch
    Insert
    Cdc42 DN (CDC42 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    T17N Dominant negative
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  23. K560_AviC_coBirA

    Plasmid
    #129765
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129765-plasmid-map-sequence-id-263583

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  24. KSP_K560GFP

    Plasmid
    #129770
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    129770-plasmid-map-sequence-id-263613

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  27. pMM0638

    Plasmid
    #129005
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    129005-plasmid-map-sequence-id-261246

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  31. K560GFP_17NL

    Plasmid
    #129767
    Purpose
    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    DAL insert before A345
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129767-plasmid-map-sequence-id-263589

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  32. pBS hTSP-3

    Plasmid
    #12995
    Depositor
    Jack Lawler
    Insert
    TSP-3 (THBS3 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 28, 2006
    Availability
    Academic Institutions and Nonprofits only

  36. pLBS.CAG

    Plasmid
    #129349
    Purpose
    Lentivirus vector
    Depositor
    Yasushi Okada
    Insert
    unspecified
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 28, 2019
    Availability
    Academic Institutions and Nonprofits only

  42. rpoDs366C

    Plasmid
    #129688
    Purpose
    Expression of Sigma 70 S366C
    Depositor
    Jeff Gelles
    Article
    Mumm et al bioRxiv 2020
    Insert
    rpoD (rpoD E. coli)
    Use
    Tags
    His6/thrombin
    Expression
    Bacterial
    Mutation
    C132S C291S C295S S366C
    Promoter
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    129688-plasmid-map-sequence-id-270569

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  43. pBN449

    Plasmid
    #129555
    Purpose
    GAL4-induced expression of codon-optimised FLP recombinase with unfused mNeongreen (mNG) as a marker in C. elegans
    Depositor
    Peter Askjaer
    Article
    Ayuso et al MicroPubl Biol. 2019 Jan 29;2019:10.1791
    Insert
    15xUAS-∆pes-10-Flp-SL2
    Use
    Tags
    mNeonGreen
    Expression
    Worm
    Mutation
    Promoter
    UAS-∆pes-10
    Available since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129555-plasmid-map-sequence-id-257130

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  47. pYPQ290

    Plasmid
    #129670
    Purpose
    Gateway entry clone for CRISPR-Cas12b systems
    Depositor
    Yiping Qi
    Article
    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:
    Insert
    AacCas12b
    Use
    CRISPR; Gateway compatible aaccas12b entry clone
    Tags
    Expression
    Plant
    Mutation
    AacCas12b is rice codon optimized
    Promoter
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    129670-plasmid-map-sequence-id-255066

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Showing: 201 - 250 of 5443 results