We narrowed to 106 results for: gfp donor template

1. pRepair-SGFP2-CTNNB1

   Plasmid

   #153430

   Purpose

   Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
   

   Use

   CRISPR; Hdr donor template

   Tags

   SGFP2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SOX2 KO (T2A-H2B-EGFP)

   Plasmid

   #167969

   Purpose

   SOX2 CDS replacement with T2A-H2B-EGFP

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   SRY-Box Transcription Factor 2 (SOX2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   H2B-EGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro

   Plasmid

   #89992

   Purpose

   Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

   Insert

   OCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AICSDP-24:MYH10-mEGFP

   Plasmid

   #87428

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   MYH10 Homology Arms with mEGFP-linker (MYH10 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   May 2, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. AICSDP-1:PXN-EGFP

   Plasmid

   #87420

   Purpose

   Homology arms and linker-EGFP sequence for C-terminus tagging of human PXN

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   PXN Homology Arms with linker-EGFP (PXN Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-EGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. AICSDP-23:TJP1-mEGFP

   Plasmid

   #87429

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 21, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. AICSDP-34: TNNI1-mEGFP

   Plasmid

   #114411

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

   Insert

   TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AICSDP-7:SEC61B-mEGFP

   Plasmid

   #87426

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. AICSDP-10:LMNB1-mEGFP

   Plasmid

   #87422

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. AICSDP-8:TOMM20-mEGFP

    Plasmid

    #87423

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. AICSDP-4:TUBA1B-mEGFP

    Plasmid

    #87421

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. AICSDP-9:DSP-mEGFP

    Plasmid

    #87424

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DSP

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    DSP Homology Arms with linker-mEGFP (DSP Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. AICSDP-49: MYL2-mEGFP

    Plasmid

    #114414

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AICSDP-13:FBL-mEGFP

    Plasmid

    #87427

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human FBL

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    FBL Homology Arms with linker-mEGFP (FBL Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. AICSDP-46: MYL7-mEGFP

    Plasmid

    #114413

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL7, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    MYL7 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL7 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDD372

    Plasmid

    #91824

    Purpose

    Codon-optimized GFP donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    GFP-C1
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AICSDP-15:ACTB-mEGFP

    Plasmid

    #87425

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. AICSDP-45: TTN-mEGFP

    Plasmid

    #114412

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    TTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDD401

    Plasmid

    #91828

    Purpose

    Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Pmyo-2::GFP
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Nucleotide 489 of the myo-2 promoter was changed …

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAP1638

    Plasmid

    #137089

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    4*(GFP11)
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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